Detailed information NTG0107251

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: RE428_RS04780
• Genome accession: NZ_AP028878
• Coordinates: 1044459..1045526 (+)
• Length: 355 a.a.
• NCBI ID: WP_004580835.1
• Uniprot ID: N6VQX1
• Organism: Marinobacter nanhaiticus D15-8W
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1039459..1050526

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RE428_RS04765 (RE428_09580)	rpmE	1039910..1040128 (+)	219	WP_004580832.1	50S ribosomal protein L31	-
RE428_RS04770 (RE428_09590)	-	1040452..1041717 (+)	1266	WP_004580833.1	malic enzyme-like NAD(P)-binding protein	-
RE428_RS04775 (RE428_09600)	-	1041795..1044263 (-)	2469	WP_004580834.1	penicillin-binding protein 1A	-
RE428_RS04780 (RE428_09610)	pilM	1044459..1045526 (+)	1068	WP_004580835.1	pilus assembly protein PilM	Machinery gene
RE428_RS04785 (RE428_09620)	-	1045526..1046092 (+)	567	WP_004580836.1	PilN domain-containing protein	-
RE428_RS04790 (RE428_09630)	pilO	1046089..1046700 (+)	612	WP_004580837.1	type 4a pilus biogenesis protein PilO	-
RE428_RS04795 (RE428_09640)	-	1046703..1047251 (+)	549	WP_040882494.1	pilus assembly protein PilP	-
RE428_RS04800 (RE428_09650)	pilQ	1047263..1049389 (+)	2127	WP_040882496.1	type IV pilus secretin PilQ	Machinery gene
RE428_RS04805 (RE428_09660)	aroK	1049523..1050089 (+)	567	WP_040882498.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 355 a.a.
• Molecular weight: 38036.18 Da
• Isoelectric Point: 4.1452

>NTDB_id=107251 RE428_RS04780 WP_004580835.1 1044459..1045526(+) (pilM) [Marinobacter nanhaiticus D15-8W]
MFGLFGKKSSTVLGIDISSSSVKLLELSRLSGERYKVESYAVEPLPANAVVEKNITDVEAVGDALKKVSGKARTSVKQAA
VAVSGSAVITKLIQMSSGLNEFEMEDQIALEADQYIPYPLDEVAIDFEVQGPSETNPDQVDVLLAACRKENVDIREDALS
IANLSAKVVDVEAYALERAYELIEPQLDAQGEELVVAIVDIGATMTTLSVLADGKTVYTREQIFGGKQLTEEIQRRYGLS
LEEAGLAKKQGGLPDDYEIEVLAPFREAVVQQVARALQFFFGASQYNAVDYVVLAGGTASIIGLTEMVEEKTGTTTLVAN
PFADMAVGSRVNASALSNDAPSLMIACGLAMRSFD

Nucleotide

• Length: 1068 bp

>NTDB_id=107251 RE428_RS04780 WP_004580835.1 1044459..1045526(+) (pilM) [Marinobacter nanhaiticus D15-8W]
GTGTTCGGATTGTTCGGTAAAAAGTCCAGTACCGTTCTGGGCATCGACATCAGTTCAAGCTCGGTCAAGCTGCTGGAACT
TTCCAGGCTGAGTGGCGAGCGGTATAAGGTGGAAAGTTATGCGGTAGAGCCGCTGCCCGCCAATGCTGTAGTCGAAAAAA
ATATCACGGATGTTGAAGCGGTCGGTGATGCGCTAAAAAAGGTGTCGGGCAAGGCTCGCACTAGCGTAAAACAGGCTGCG
GTTGCTGTTTCGGGGTCGGCGGTTATTACCAAGCTGATCCAGATGAGCAGTGGCCTCAACGAATTTGAAATGGAAGACCA
GATCGCGCTGGAGGCCGATCAGTACATTCCTTATCCCCTTGATGAAGTCGCCATCGACTTCGAGGTCCAGGGGCCTTCGG
AAACCAATCCCGATCAGGTCGATGTCCTTCTGGCTGCGTGCCGGAAAGAGAACGTCGACATCCGGGAGGACGCTCTTTCC
ATTGCCAATCTGTCCGCCAAGGTCGTCGATGTCGAAGCCTACGCCCTTGAGCGCGCCTACGAGTTGATCGAACCGCAGTT
GGATGCGCAGGGCGAAGAGTTGGTCGTGGCCATCGTAGATATCGGTGCGACCATGACGACCCTGAGTGTGCTTGCCGACG
GCAAGACGGTCTATACCCGAGAGCAGATTTTCGGTGGCAAGCAGTTGACCGAAGAAATCCAGCGTCGCTATGGCCTGTCC
CTGGAAGAGGCCGGTCTTGCAAAGAAACAGGGTGGCTTGCCGGACGACTACGAAATAGAGGTGCTGGCACCTTTCCGTGA
GGCAGTCGTTCAACAGGTCGCTCGTGCGCTGCAGTTCTTCTTCGGCGCGAGTCAGTACAACGCAGTCGACTACGTCGTGC
TTGCCGGCGGCACGGCTTCCATTATCGGTCTGACCGAAATGGTGGAAGAAAAGACGGGCACGACAACGCTGGTCGCGAAT
CCCTTTGCGGATATGGCGGTGGGTTCCCGCGTCAATGCGTCTGCCCTGAGTAACGATGCGCCGTCACTGATGATTGCCTG
TGGTCTGGCGATGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-354)

3D structure

• AlphaFold DB: N6VQX1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	53.803	100	0.538
comM	Acinetobacter nosocomialis M2	53.803	100	0.538
comM	Acinetobacter baylyi ADP1	51.268	100	0.513
pilM	Legionella pneumophila strain ERS1305867	46.761	100	0.468