Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   AAA995_RS09825 Genome accession   NZ_AP028877
Coordinates   1899316..1900332 (-) Length   338 a.a.
NCBI ID   WP_078130737.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain 21P20     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1894316..1905332
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAA995_RS09780 - 1894399..1894530 (-) 132 WP_000768904.1 hypothetical protein -
  AAA995_RS09785 (SP21P20_19130) - 1894531..1895721 (-) 1191 WP_000167765.1 acetate kinase -
  AAA995_RS09790 (SP21P20_19140) comYH 1895772..1896725 (-) 954 WP_000345126.1 class I SAM-dependent methyltransferase Machinery gene
  AAA995_RS09795 - 1896786..1897378 (-) 593 Protein_1904 methyltransferase domain-containing protein -
  AAA995_RS09800 (SP21P20_19170) comGG/cglG 1897481..1897927 (-) 447 WP_001809944.1 competence type IV pilus minor pilin ComGG Machinery gene
  AAA995_RS09805 (SP21P20_19180) comGF/cglF 1897905..1898366 (-) 462 WP_000250531.1 competence type IV pilus minor pilin ComGF Machinery gene
  AAA995_RS09810 (SP21P20_19190) comGE/cglE 1898329..1898631 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  AAA995_RS09815 (SP21P20_19200) comGD/cglD 1898594..1899028 (-) 435 WP_033606693.1 competence type IV pilus minor pilin ComGD Machinery gene
  AAA995_RS09820 (SP21P20_19210) comGC/cglC 1898991..1899314 (-) 324 WP_000738628.1 comG operon protein ComGC Machinery gene
  AAA995_RS09825 (SP21P20_19220) comGB/cglB 1899316..1900332 (-) 1017 WP_078130737.1 competence type IV pilus assembly protein ComGB Machinery gene
  AAA995_RS09830 (SP21P20_19230) comGA/cglA/cilD 1900280..1901221 (-) 942 WP_338173922.1 competence type IV pilus ATPase ComGA Machinery gene
  AAA995_RS09835 (SP21P20_19240) - 1901297..1901662 (-) 366 WP_000286415.1 DUF1033 family protein -
  AAA995_RS09840 (SP21P20_19250) - 1901813..1902871 (-) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -
  AAA995_RS09845 (SP21P20_19260) nagA 1903034..1904185 (-) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38523.66 Da        Isoelectric Point: 9.8240

>NTDB_id=107230 AAA995_RS09825 WP_078130737.1 1899316..1900332(-) (comGB/cglB) [Streptococcus pneumoniae strain 21P20]
MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSALLDKQCVTQMRVGLSQGKSFSEMMKSLGCSSAI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLFFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEI
GQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=107230 AAA995_RS09825 WP_078130737.1 1899316..1900332(-) (comGB/cglB) [Streptococcus pneumoniae strain 21P20]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATCTCTTTTCTAGCGGTTTTCATCTGGTGGAGACTATCTCCTTTTTAGATAGGAGTGCTTTGTTGGACAAGCAGTGTG
TGACCCAGATGCGTGTGGGCTTGTCTCAGGGGAAATCATTCTCAGAAATGATGAAAAGTTTGGGATGTTCAAGTGCTATT
GTCACTCAGTTATCCCTAGCTGAAGTCCATGGTAATCTCCACCTGAGTTTGGGAAAGATAGAAGAGTATCTAGACAATCT
GGCCAAGGTCAAGAAAAAATTAATTGAAGTAGCGACCTATCCTTTGATTTTGCTGTTTTTTCTTCTCTTAATTATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCGAATTATCGGTAATCTGCCACAAATTTTT
CTAGGCATGGTAGGGCTTGTTTCCGTGCTTGCCCTTTTAGCACTAACTTTTTATAAAAGAAGTTCTAAGATGAGTGTCTT
TTCTATCTTAGCACGCCTTCCCTTTATTGGAATCTTTGTGCAGACCTACTTGACAGCCTATTATGCACGTGAATGGGGGA
ATATGATTTCACAGGGAATGGAGCTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAATC
GGTCAAGATCTGGCTCAAACCCTGAAAAATGGCCGTGAATTTTCTCAGACGATAGGAACCTATCCTTTCTTTAGGAAGGA
ATTGAGTCTCATCATAGAGTATGGGGAAGTTAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATTTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae Rx1

98.817

100

0.988
  comGB/cglB Streptococcus pneumoniae D39

98.817

100

0.988
  comGB/cglB Streptococcus pneumoniae R6

98.817

100

0.988
  comGB/cglB Streptococcus pneumoniae TIGR4

98.817

100

0.988
  comGB/cglB Streptococcus mitis SK321

94.675

100

0.947
  comGB/cglB Streptococcus mitis NCTC 12261

94.083

100

0.941
  comYB Streptococcus gordonii str. Challis substr. CH1

70.833

99.408

0.704
  comYB Streptococcus mutans UA140

58.176

94.083

0.547
  comYB Streptococcus mutans UA159

58.176

94.083

0.547
  comGB Lactococcus lactis subsp. cremoris KW2

50.599

98.817

0.5

Multiple sequence alignment