Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACI3L8_RS10160 Genome accession   NZ_CP173391
Coordinates   2200072..2201757 (-) Length   561 a.a.
NCBI ID   WP_404971380.1    Uniprot ID   -
Organism   Vibrio campbellii strain LB3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2195072..2206757
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACI3L8_RS10130 (ACI3L8_10130) rplS 2195419..2195772 (+) 354 WP_005379971.1 50S ribosomal protein L19 -
  ACI3L8_RS10135 (ACI3L8_10135) yacG 2196232..2196426 (-) 195 WP_005534053.1 DNA gyrase inhibitor YacG -
  ACI3L8_RS10140 (ACI3L8_10140) zapD 2196489..2197229 (-) 741 WP_012128881.1 cell division protein ZapD -
  ACI3L8_RS10145 (ACI3L8_10145) coaE 2197257..2197871 (-) 615 WP_012128880.1 dephospho-CoA kinase -
  ACI3L8_RS10150 (ACI3L8_10150) pilD 2197872..2198741 (-) 870 WP_045381296.1 A24 family peptidase Machinery gene
  ACI3L8_RS10155 (ACI3L8_10155) pilC 2198814..2200037 (-) 1224 WP_404971379.1 type II secretion system F family protein Machinery gene
  ACI3L8_RS10160 (ACI3L8_10160) pilB 2200072..2201757 (-) 1686 WP_404971380.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACI3L8_RS10165 (ACI3L8_10165) - 2201761..2202240 (-) 480 WP_103412702.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ACI3L8_RS10170 (ACI3L8_10170) nadC 2202506..2203393 (-) 888 WP_404971381.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACI3L8_RS10175 (ACI3L8_10175) ampD 2203486..2204037 (+) 552 WP_404971382.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACI3L8_RS10180 (ACI3L8_10180) pdhR 2204442..2205209 (+) 768 WP_005428674.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62096.85 Da        Isoelectric Point: 5.1024

>NTDB_id=1072031 ACI3L8_RS10160 WP_404971380.1 2200072..2201757(-) (pilB) [Vibrio campbellii strain LB3]
MLTNLSTVLRQAGILTFSQEESLLEQVKASGISMPEALLSSGFFQSHELTEHLSSVFGLSCTSLNQYEYSSLCQTLGLRD
LITRHNALPLNRTSSTLILAVADPTNLQAEDDFRFATGLQVELVLADFRELTAAIRRLYGRSLGQEKSGLKEINQDELAG
LVDIGEDEIENIEDLSQDESPVSRYINQILLDAVRKGASDIHFEPYENMYRVRLRCDGILIEIQQPPSHLSRRLSARIKI
LSKLDIAERRLPQDGRIKLKLNQDTAIDMRVSTLPTLFGEKIVLRLLDSSSAALDINKLGYSDSQKQLYLDALRRPQGMI
LMTGPTGSGKTVSLYTGLSILNKPEINISTAEDPVEINLPGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLDT
AEIAIKASQTGHLVLSTLHTNSAAETVIRLSNMGVESFNLASSLSLIIAQRLARKLCPYCRQPQEPNSQLQHIGITSTEQ
IFQANPDGCNECTHGYSGRTGIYEVMKFDESLSEALIKGASVHELEKLAIANGMQTLQMSGIEKLKQGITSFSELQRVLY
F

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=1072031 ACI3L8_RS10160 WP_404971380.1 2200072..2201757(-) (pilB) [Vibrio campbellii strain LB3]
ATGCTAACCAACCTCTCAACGGTTCTTCGCCAAGCTGGAATACTGACCTTTTCCCAAGAAGAATCGTTACTTGAACAAGT
AAAAGCTTCTGGCATTTCGATGCCGGAGGCTTTACTCAGTTCTGGCTTTTTCCAATCTCATGAATTAACGGAACACCTAA
GCTCAGTATTCGGGCTTTCTTGCACTTCTCTTAACCAATACGAATACTCTTCGCTATGCCAAACGCTTGGCTTACGCGAT
CTCATCACTCGCCATAACGCTTTGCCGCTTAACCGCACCTCTTCAACTTTGATTCTCGCGGTTGCTGACCCAACAAACCT
TCAAGCCGAAGATGACTTTCGTTTTGCGACAGGTTTGCAAGTCGAACTTGTCCTCGCTGACTTTCGCGAACTGACAGCAG
CCATTCGGCGTTTATATGGCCGATCGCTTGGGCAAGAAAAATCCGGTCTTAAAGAAATCAACCAAGATGAACTGGCGGGT
TTGGTTGATATTGGTGAGGATGAAATTGAGAACATCGAAGATCTCAGCCAAGATGAATCGCCGGTCAGTCGCTATATCAA
CCAAATTTTACTCGATGCTGTCCGTAAAGGGGCTTCCGATATTCACTTCGAGCCCTATGAAAACATGTACCGCGTGCGCT
TGCGTTGCGATGGCATCTTAATTGAAATTCAGCAGCCTCCGAGTCATTTAAGCCGTCGCCTTTCAGCTCGGATAAAAATC
TTATCTAAGCTTGATATCGCCGAGCGCCGTTTACCACAGGATGGGCGAATCAAGCTCAAATTAAACCAAGACACTGCAAT
TGATATGCGTGTCTCCACACTACCAACTCTGTTTGGGGAAAAGATTGTTCTGCGTTTGCTCGACAGCAGTTCTGCGGCAT
TAGATATCAATAAGCTCGGTTATAGCGACTCACAAAAACAACTTTATTTGGATGCCCTGCGCCGTCCGCAAGGTATGATC
CTGATGACAGGCCCAACAGGTAGCGGTAAAACGGTCTCGCTCTATACTGGATTGAGTATTCTTAATAAGCCAGAGATCAA
TATCTCAACCGCTGAAGATCCCGTTGAGATTAACCTGCCCGGCATTAACCAAGTTCAAGTTCAGCCAAAGATCGGCTTTG
GTTTTGCAGAAGCGCTCCGCTCTTTCTTACGTCAAGACCCTGATGTAGTCATGGTGGGCGAGATCCGAGATCTCGACACA
GCAGAAATCGCGATCAAAGCATCACAGACTGGCCACTTAGTTCTCTCCACTTTACACACAAACTCAGCTGCCGAGACTGT
TATTCGCTTGTCTAATATGGGCGTAGAAAGCTTTAACCTCGCCTCTTCTCTCAGTTTGATCATCGCCCAGCGTTTGGCTA
GAAAACTTTGTCCATATTGTAGACAACCTCAAGAGCCAAACAGCCAACTTCAACATATTGGAATCACCAGCACCGAACAG
ATCTTTCAAGCTAATCCCGACGGTTGTAACGAGTGTACCCATGGTTATTCAGGGCGAACCGGCATTTACGAAGTGATGAA
ATTTGATGAATCCCTTTCAGAAGCACTCATCAAAGGCGCTTCAGTACACGAATTAGAAAAACTCGCCATTGCCAATGGTA
TGCAAACCCTACAAATGTCTGGTATCGAAAAACTCAAACAAGGCATTACCAGCTTCAGCGAATTGCAGCGCGTTCTCTAC
TTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-142)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

99.109

100

0.991
  pilB Vibrio parahaemolyticus RIMD 2210633

90.553

100

0.906
  pilB Vibrio cholerae strain A1552

73.488

100

0.736
  pilB Acinetobacter baumannii D1279779

51.445

92.513

0.476
  pilB Legionella pneumophila strain ERS1305867

47.756

99.287

0.474
  pilB Acinetobacter baylyi ADP1

50.097

92.157

0.462
  pilF Neisseria gonorrhoeae MS11

43.617

100

0.439
  pilB/pilB1 Synechocystis sp. PCC 6803

37.037

100

0.392
  pilF Thermus thermophilus HB27

36.833

100

0.369
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.559

99.465

0.364

Multiple sequence alignment