Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACINK7_RS19015 Genome accession   NZ_CP173170
Coordinates   4376346..4378064 (-) Length   572 a.a.
NCBI ID   WP_404357733.1    Uniprot ID   -
Organism   Methylotuvimicrobium sp. KM1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4371346..4383064
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACINK7_RS18985 (ACINK7_18985) yacG 4372141..4372347 (-) 207 WP_404357723.1 DNA gyrase inhibitor YacG -
  ACINK7_RS18990 (ACINK7_18990) zapD 4372347..4373117 (-) 771 WP_014147183.1 cell division protein ZapD -
  ACINK7_RS18995 (ACINK7_18995) coaE 4373121..4373726 (-) 606 WP_404357725.1 dephospho-CoA kinase -
  ACINK7_RS19000 (ACINK7_19000) pilD 4373731..4374633 (-) 903 WP_404357727.1 prepilin peptidase Machinery gene
  ACINK7_RS19005 (ACINK7_19005) - 4374658..4374864 (-) 207 WP_404357729.1 hypothetical protein -
  ACINK7_RS19010 (ACINK7_19010) pilC 4375123..4376343 (-) 1221 WP_404357731.1 type II secretion system F family protein Machinery gene
  ACINK7_RS19015 (ACINK7_19015) pilB 4376346..4378064 (-) 1719 WP_404357733.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACINK7_RS19020 (ACINK7_19020) - 4378175..4380985 (-) 2811 WP_404357735.1 valine--tRNA ligase -
  ACINK7_RS19025 (ACINK7_19025) - 4381051..4381503 (-) 453 WP_404357737.1 DNA polymerase III subunit chi -
  ACINK7_RS19030 (ACINK7_19030) - 4381491..4382978 (-) 1488 WP_404357739.1 leucyl aminopeptidase -

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 62768.65 Da        Isoelectric Point: 6.0961

>NTDB_id=1070401 ACINK7_RS19015 WP_404357733.1 4376346..4378064(-) (pilB) [Methylotuvimicrobium sp. KM1]
MATDLIDLPIGGLPKCLVQAGLITEADMQLHMQAAKKAKLPLISYLVTNKLVDSKSVTAKASKEFGIPQLDLDALDIRAL
PVNLVSEKLIRKHHVLPLLKRGNRLFLAVSDPGNFQAQDEIKFHTRLNIDTILVEEDKLSKAIETSLEAADTTMTDLLDS
DLDNLEITGGDQEPAGDEAASDVDDAPIVRFVNKILLDSIKKGVSDIHLEPYEKSFRIRFRADGMLYEVASPPPNISARI
ISRIKVMAKMDIAERRVPQDGRIKMALSKNRAIDFRVNSCPTLFGEKVVLRILDPTSAQIGIEKLGFEPVQQQRFLEAIN
RPYGMVLVTGPTGSGKTVTLYTGLNLLNSVERNISTAEDPVEITVPGINQVNMNPKAGLTFAGALRAFLRQDPDIIMVGE
IRDLETAEIAVKAAQTGHLVLSTLHTNDAPQTLNRLVQMGVPPFNIVSAVHLIMAQRLARRLCEYCKVKSDLPDSVLISA
GFKEEDLTKIKIFTANPAGCEHCTSGYKGRVGIYQVMPISENMRKLILEGGNTMQLAEQAKAEGINDLRASGLEKVCQGI
TSLEEIDRVTKE

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=1070401 ACINK7_RS19015 WP_404357733.1 4376346..4378064(-) (pilB) [Methylotuvimicrobium sp. KM1]
ATGGCAACAGATTTAATCGACCTGCCGATCGGCGGGCTACCGAAATGTTTGGTACAAGCCGGCCTGATTACCGAGGCGGA
TATGCAGTTGCATATGCAGGCAGCGAAGAAAGCCAAATTACCGCTAATCAGTTATTTGGTGACCAATAAATTGGTTGACA
GTAAATCCGTAACAGCCAAAGCTTCCAAAGAATTCGGTATTCCGCAGCTCGATCTCGACGCGCTCGATATTCGTGCCTTG
CCAGTCAATTTAGTTAGCGAAAAACTGATCAGAAAGCATCATGTTTTACCCTTGCTTAAGCGCGGAAACCGGTTGTTTTT
GGCGGTTTCCGACCCCGGCAATTTTCAAGCACAGGACGAGATAAAGTTTCATACGCGCCTGAATATCGACACGATTCTGG
TCGAAGAAGATAAATTGTCTAAGGCTATCGAGACGTCGCTGGAAGCTGCCGATACCACGATGACCGATTTACTGGATTCC
GATCTGGATAATCTCGAAATTACCGGCGGCGATCAGGAGCCGGCCGGAGACGAGGCTGCAAGCGATGTGGACGATGCGCC
TATCGTTCGCTTCGTTAATAAAATTTTGCTCGATTCGATTAAAAAAGGGGTTTCGGACATTCATCTTGAGCCTTATGAAA
AAAGCTTTCGAATTCGCTTTCGCGCAGACGGCATGCTATATGAAGTGGCAAGTCCGCCTCCCAATATCTCGGCCCGGATT
ATTTCTCGTATCAAGGTCATGGCTAAAATGGATATCGCCGAACGGCGTGTTCCTCAAGACGGACGGATTAAGATGGCGTT
GTCGAAAAATCGGGCAATCGATTTTCGGGTCAATAGTTGTCCGACTTTGTTCGGTGAAAAAGTCGTACTGCGTATTCTCG
ATCCGACCAGCGCGCAAATCGGCATCGAAAAACTCGGTTTTGAACCCGTCCAACAACAACGATTTCTAGAGGCGATCAAC
CGGCCTTATGGTATGGTTTTGGTAACAGGGCCTACAGGTAGCGGTAAAACCGTTACCCTTTACACAGGCTTGAATCTGCT
AAATAGCGTCGAACGGAATATATCGACTGCCGAGGATCCGGTTGAAATTACGGTGCCGGGTATCAATCAGGTCAATATGA
ATCCAAAAGCCGGATTGACCTTTGCCGGTGCCTTGAGGGCTTTTCTTCGGCAAGATCCGGATATCATCATGGTCGGTGAG
ATACGGGATTTGGAAACCGCCGAGATTGCTGTCAAGGCCGCACAAACGGGCCACTTGGTATTGTCTACGCTGCACACTAA
CGATGCGCCGCAAACGTTAAATCGACTCGTGCAAATGGGCGTGCCGCCGTTTAACATAGTTTCGGCGGTACATTTGATCA
TGGCTCAACGTCTTGCTAGACGCTTATGCGAATATTGTAAGGTCAAATCCGATTTACCGGATTCGGTGTTGATTTCTGCC
GGTTTCAAAGAAGAAGATCTAACTAAGATTAAAATTTTTACGGCCAATCCTGCCGGTTGCGAACATTGCACTAGCGGTTA
CAAAGGACGGGTCGGCATTTATCAGGTTATGCCGATCAGCGAAAACATGCGGAAACTGATTCTGGAAGGCGGCAATACCA
TGCAATTGGCCGAACAAGCCAAAGCCGAAGGCATCAACGACCTGCGCGCGTCCGGCCTTGAAAAAGTCTGTCAAGGCATC
ACTAGCCTGGAAGAAATCGACCGGGTCACTAAGGAGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-145)

Type II/IV secretion system protein (T2SSE)

(194-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

57.447

98.601

0.566
  pilB Acinetobacter baumannii D1279779

53.546

98.601

0.528
  pilB Acinetobacter baylyi ADP1

52.931

98.427

0.521
  pilB Vibrio cholerae strain A1552

48.754

98.252

0.479
  pilF Neisseria gonorrhoeae MS11

47.788

98.776

0.472
  pilB Vibrio parahaemolyticus RIMD 2210633

47.772

98.077

0.469
  pilB Vibrio campbellii strain DS40M4

47.594

98.077

0.467
  pilF Thermus thermophilus HB27

38.609

100

0.388
  pilB/pilB1 Synechocystis sp. PCC 6803

42.5

90.909

0.386

Multiple sequence alignment