Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   ACINK7_RS19000 Genome accession   NZ_CP173170
Coordinates   4373731..4374633 (-) Length   300 a.a.
NCBI ID   WP_404357727.1    Uniprot ID   -
Organism   Methylotuvimicrobium sp. KM1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4368731..4379633
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACINK7_RS18975 (ACINK7_18975) - 4369381..4371135 (+) 1755 WP_404357720.1 Hsp70 family protein -
  ACINK7_RS18980 (ACINK7_18980) - 4371204..4371557 (+) 354 WP_404357722.1 hypothetical protein -
  ACINK7_RS18985 (ACINK7_18985) yacG 4372141..4372347 (-) 207 WP_404357723.1 DNA gyrase inhibitor YacG -
  ACINK7_RS18990 (ACINK7_18990) zapD 4372347..4373117 (-) 771 WP_014147183.1 cell division protein ZapD -
  ACINK7_RS18995 (ACINK7_18995) coaE 4373121..4373726 (-) 606 WP_404357725.1 dephospho-CoA kinase -
  ACINK7_RS19000 (ACINK7_19000) pilD 4373731..4374633 (-) 903 WP_404357727.1 prepilin peptidase Machinery gene
  ACINK7_RS19005 (ACINK7_19005) - 4374658..4374864 (-) 207 WP_404357729.1 hypothetical protein -
  ACINK7_RS19010 (ACINK7_19010) pilC 4375123..4376343 (-) 1221 WP_404357731.1 type II secretion system F family protein Machinery gene
  ACINK7_RS19015 (ACINK7_19015) pilB 4376346..4378064 (-) 1719 WP_404357733.1 type IV-A pilus assembly ATPase PilB Machinery gene

Sequence

Protein


Download         Length: 300 a.a.        Molecular weight: 33064.04 Da        Isoelectric Point: 5.6198

>NTDB_id=1070399 ACINK7_RS19000 WP_404357727.1 4373731..4374633(-) (pilD) [Methylotuvimicrobium sp. KM1]
MQGLEIFADSPAALMALTGVIGLMVGSFLNVVIYRLPIMMQKSWRSECIEYLKSTGDSQFAELDAGSGDADSKFNLVFPL
SRCPHCETPIKPYQNIPVFSYLLLKGCCAQCNAPISKRYPIIEALTGFFSVLIAWHFGYSIEALFALILTWSLIALSAID
IDYQLLPDSITLPLLWLGLFLSLFDIFTDTHSSIIGAIAGYLSLWTVYHGFKLLTGKEGMGYGDFKLLAMFGAWLGWQYI
LPIILLSSLVGAVTGIAMVILVKRDKNIPIPFGPYLAAAGWIALLWGDKINQAYFNFAGL

Nucleotide


Download         Length: 903 bp        

>NTDB_id=1070399 ACINK7_RS19000 WP_404357727.1 4373731..4374633(-) (pilD) [Methylotuvimicrobium sp. KM1]
ATGCAAGGACTAGAAATATTCGCCGATTCGCCGGCTGCACTAATGGCTTTAACCGGTGTTATCGGTCTCATGGTCGGTAG
TTTCCTTAACGTCGTTATTTACCGTCTGCCGATTATGATGCAAAAAAGTTGGCGCAGTGAATGCATCGAATATCTGAAGT
CTACGGGGGATTCGCAATTCGCGGAGCTCGACGCAGGAAGTGGGGACGCCGACTCAAAATTTAACTTAGTTTTTCCGTTG
TCACGCTGTCCCCATTGCGAAACCCCGATAAAGCCTTATCAGAATATTCCGGTATTTAGTTATTTGTTGTTGAAAGGGTG
TTGCGCTCAATGCAATGCGCCGATATCGAAACGCTATCCCATTATCGAGGCTTTAACCGGTTTTTTTTCGGTGTTAATCG
CCTGGCATTTCGGTTATTCGATAGAGGCATTATTCGCGCTCATACTCACCTGGTCCTTGATTGCGCTGAGTGCGATCGAC
ATTGACTATCAACTGTTGCCGGACTCGATTACGTTACCTTTGCTATGGTTGGGTCTGTTTTTAAGCCTGTTCGATATCTT
CACCGATACACATTCCTCGATTATTGGTGCTATCGCCGGTTATTTATCGCTTTGGACGGTTTATCATGGTTTCAAGCTGC
TGACCGGCAAAGAGGGCATGGGATACGGCGACTTTAAGCTGTTGGCAATGTTTGGGGCATGGTTGGGTTGGCAATATATC
CTGCCGATCATTTTGCTGTCATCCTTGGTCGGTGCCGTTACCGGCATCGCGATGGTTATTTTAGTTAAGCGCGACAAGAA
TATTCCAATTCCATTCGGCCCTTATTTGGCAGCGGCCGGGTGGATCGCTTTACTGTGGGGCGATAAAATCAATCAGGCTT
ATTTCAATTTTGCCGGATTATAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(147-256)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

51.02

98

0.5
  pilD Vibrio campbellii strain DS40M4

50.34

98

0.493
  pilD Acinetobacter baumannii D1279779

49.481

96.333

0.477
  pilD Acinetobacter nosocomialis M2

49.481

96.333

0.477
  pilD Neisseria gonorrhoeae MS11

47.887

94.667

0.453

Multiple sequence alignment