Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACIHQS_RS16210 Genome accession   NZ_CP173025
Coordinates   3411684..3412862 (-) Length   392 a.a.
NCBI ID   WP_004882232.1    Uniprot ID   N8YGL0
Organism   Acinetobacter venetianus strain AVYS1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3406684..3417862
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACIHQS_RS16180 (ACIHQS_16180) - 3406742..3407251 (+) 510 WP_404229323.1 Bro-N domain-containing protein -
  ACIHQS_RS16185 (ACIHQS_16185) - 3407244..3407411 (+) 168 WP_404229325.1 hypothetical protein -
  ACIHQS_RS16190 (ACIHQS_16190) - 3407408..3407830 (+) 423 Protein_3149 hypothetical protein -
  ACIHQS_RS16195 (ACIHQS_16195) - 3407897..3409047 (+) 1151 Protein_3150 IS3 family transposase -
  ACIHQS_RS16200 (ACIHQS_16200) - 3410443..3410745 (+) 303 WP_404231756.1 hypothetical protein -
  ACIHQS_RS16205 (ACIHQS_16205) - 3410823..3411635 (+) 813 WP_404229328.1 DUF2310 family Zn-ribbon-containing protein -
  ACIHQS_RS16210 (ACIHQS_16210) pilU 3411684..3412862 (-) 1179 WP_004882232.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACIHQS_RS16215 (ACIHQS_16215) - 3413302..3414000 (+) 699 WP_404229330.1 DsbC family protein -
  ACIHQS_RS16220 (ACIHQS_16220) - 3414052..3414303 (-) 252 WP_023012777.1 GlsB/YeaQ/YmgE family stress response membrane protein -
  ACIHQS_RS16225 (ACIHQS_16225) hpt 3414542..3415072 (-) 531 WP_023012778.1 hypoxanthine phosphoribosyltransferase -
  ACIHQS_RS16230 (ACIHQS_16230) guaD 3415298..3416614 (-) 1317 WP_404229334.1 guanine deaminase -
  ACIHQS_RS16235 (ACIHQS_16235) - 3416749..3417375 (+) 627 WP_257736526.1 nucleotidyltransferase family protein -

Sequence

Protein


Download         Length: 392 a.a.        Molecular weight: 44369.90 Da        Isoelectric Point: 6.4468

>NTDB_id=1070016 ACIHQS_RS16210 WP_004882232.1 3411684..3412862(-) (pilU) [Acinetobacter venetianus strain AVYS1]
MYNAELLEEAKTFMFHMLTKVVEYGGSDLFITADFPPSIKLQGLMRPLGQQALTADKTKLFAYSLMNEKQRQEFENEWEC
NFAINVPNVSRFRVNVFKQQLQIGMVIRTITSEIPTFQKLKLPESLKNVIMEKRGLVLVVGGTGSGKSTSLAAMIDHRNE
NSAGHIITVEDPVEYVHKHKKSMITHREVGVDCHSWHHALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
ANNANQALDRIINFFPEERRNQLLMDLSSNMKAIISQRLVRTEDGKGRRAAIEILLNTPLIADNILKGQFHVLKEIMSKS
RELGMQTFDQALFDLYNEGAISYDEALRNADSVNELRLQIKLKSNRQEGVPVAATALNMVKEEEKAEEQEEA

Nucleotide


Download         Length: 1179 bp        

>NTDB_id=1070016 ACIHQS_RS16210 WP_004882232.1 3411684..3412862(-) (pilU) [Acinetobacter venetianus strain AVYS1]
ATGTACAATGCTGAATTATTAGAAGAAGCCAAAACCTTTATGTTCCATATGCTCACTAAAGTTGTGGAATATGGTGGCTC
TGATTTATTTATAACCGCTGATTTTCCGCCGAGTATTAAATTACAAGGATTAATGCGACCTTTGGGGCAGCAGGCTCTAA
CAGCTGATAAAACCAAATTATTTGCTTATAGTTTAATGAATGAAAAGCAAAGACAAGAATTTGAAAATGAATGGGAATGT
AATTTCGCCATTAATGTTCCAAATGTTTCACGTTTCCGCGTCAATGTATTCAAACAACAACTTCAAATCGGGATGGTCAT
TCGTACCATTACCTCTGAAATTCCAACCTTTCAAAAACTAAAACTACCAGAATCACTTAAAAATGTGATTATGGAAAAGC
GTGGTTTAGTATTGGTCGTTGGCGGTACTGGTTCAGGTAAGTCGACCTCTTTAGCAGCCATGATTGATCATCGCAATGAG
AATTCAGCAGGTCATATCATCACCGTTGAAGACCCTGTGGAATATGTACACAAGCACAAAAAATCGATGATTACGCATCG
CGAGGTTGGCGTTGATTGCCACTCATGGCATCACGCTTTGAAAAACACCTTACGCCAAGCGCCTGATGTCATTCTGATTG
GAGAGATCCGCGATACTGAAACCATGGAACATGCGATTGCTTTTGCGGAAACAGGACATCTCTGTTTAGGGACTTTACAC
GCAAATAATGCCAACCAAGCCTTAGATCGTATTATTAACTTCTTCCCTGAAGAGCGCCGTAACCAATTGCTGATGGATTT
ATCATCCAATATGAAAGCCATTATTTCACAGCGCTTAGTTCGTACTGAGGATGGTAAAGGTCGTCGTGCAGCAATTGAGA
TTCTACTCAATACGCCATTGATTGCAGATAATATTCTAAAAGGACAATTCCATGTGCTTAAAGAAATTATGAGCAAATCA
CGTGAATTGGGTATGCAAACGTTTGATCAGGCCCTATTTGATCTCTATAACGAAGGCGCGATTAGCTATGATGAGGCGCT
GCGAAATGCAGATTCAGTCAATGAATTACGCTTACAAATCAAATTGAAAAGTAACCGCCAAGAGGGTGTCCCTGTCGCTG
CAACGGCACTGAATATGGTGAAAGAAGAAGAAAAAGCTGAAGAGCAAGAAGAAGCTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(103-282)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N8YGL0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.92

88.776

0.541
  pilU Acinetobacter baylyi ADP1

56.484

88.52

0.5
  pilU Vibrio cholerae strain A1552

52.841

89.796

0.474
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.814

85.204

0.365
  pilT Acinetobacter baylyi ADP1

42.262

85.714

0.362
  pilT Acinetobacter baumannii D1279779

43.558

83.163

0.362
  pilT Acinetobacter baumannii strain A118

43.558

83.163

0.362
  pilT Acinetobacter nosocomialis M2

43.558

83.163

0.362

Multiple sequence alignment