Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACIHQS_RS01105 Genome accession   NZ_CP173025
Coordinates   229482..231200 (-) Length   572 a.a.
NCBI ID   WP_404229820.1    Uniprot ID   -
Organism   Acinetobacter venetianus strain AVYS1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 224482..236200
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACIHQS_RS01075 (ACIHQS_01075) - 224580..224906 (+) 327 WP_004876475.1 pyrimidine/purine nucleoside phosphorylase -
  ACIHQS_RS01080 (ACIHQS_01080) rlmB 225075..225824 (+) 750 WP_004876476.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  ACIHQS_RS01085 (ACIHQS_01085) - 225856..226767 (+) 912 WP_007480121.1 DMT family transporter -
  ACIHQS_RS01090 (ACIHQS_01090) coaE 226764..227369 (-) 606 WP_404229814.1 dephospho-CoA kinase -
  ACIHQS_RS01095 (ACIHQS_01095) pilD 227371..228231 (-) 861 WP_404229817.1 prepilin peptidase Machinery gene
  ACIHQS_RS01100 (ACIHQS_01100) pilC 228231..229457 (-) 1227 WP_061397953.1 type II secretion system F family protein Machinery gene
  ACIHQS_RS01105 (ACIHQS_01105) pilB 229482..231200 (-) 1719 WP_404229820.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACIHQS_RS01110 (ACIHQS_01110) tpiA 231555..232346 (+) 792 WP_404229823.1 triose-phosphate isomerase -
  ACIHQS_RS01115 (ACIHQS_01115) secG 232359..232688 (+) 330 WP_404229826.1 preprotein translocase subunit SecG -
  ACIHQS_RS01135 (ACIHQS_01135) rimP 233310..233834 (+) 525 WP_404229829.1 ribosome maturation factor RimP -
  ACIHQS_RS01140 (ACIHQS_01140) nusA 233872..235356 (+) 1485 WP_004876494.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 63497.84 Da        Isoelectric Point: 5.0825

>NTDB_id=1069987 ACIHQS_RS01105 WP_404229820.1 229482..231200(-) (pilB) [Acinetobacter venetianus strain AVYS1]
MSGLHTSPKFSGFIRRLVDDGHLTASNMQTAIDAAKKAKQDIVAHLIEHQRISPLIIAESISLEFGEPLFDLAVYDTAIL
PREITDQKLIQRHRIVPLVQRGQILYVATSNPTNIDALDAIRFNTKLNIEPIIVEHNKLEKLIEQQFGDTGSFDFGDGEE
FDLEGIDVESDTSQDEEDTSNQGDESPIVKYINKLLIDAIRMGASDLHFEPYEKMYRVRYRVDGVLRQIANPPLQMANRL
ASRLKVMSQMDISEKRMPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIEALGYEDEQKALFLEALE
KPQGMLLITGPTGSGKTVSLYTGLNILNTEDTNISTAEDPVEINLEGINQVNVNPKTGLTFSAALKAFLRQDPDIIMVGE
VRDLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCQQCKVPVDIPKQSLIEL
GFTEQDLADPELKLFQPVGCSECREGYKGRVGIYEVMKVTPEISKIIMEDGNALQIAAASEKAGFNNLRRSGLKKVMQGI
TSLQEVNRVTSE

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=1069987 ACIHQS_RS01105 WP_404229820.1 229482..231200(-) (pilB) [Acinetobacter venetianus strain AVYS1]
ATGTCAGGATTACATACGTCTCCAAAGTTTTCGGGGTTTATTCGACGTTTAGTCGATGATGGTCATTTGACTGCGTCAAA
TATGCAAACCGCAATTGATGCTGCAAAAAAGGCTAAACAAGATATTGTTGCTCATCTGATTGAGCACCAACGAATATCGC
CTCTAATAATTGCGGAAAGCATTTCACTCGAGTTTGGTGAACCACTATTTGATTTAGCTGTTTACGACACAGCCATTCTT
CCTCGCGAAATTACAGATCAAAAACTAATCCAAAGACATCGAATTGTGCCTTTGGTACAGCGTGGTCAAATCTTATATGT
AGCAACCAGCAACCCCACCAATATTGATGCCTTAGATGCAATTCGCTTCAATACTAAACTTAATATTGAACCGATTATTG
TTGAACATAACAAATTAGAAAAACTGATTGAACAACAATTTGGTGACACGGGTAGCTTTGATTTTGGTGATGGTGAAGAG
TTTGATTTAGAGGGTATTGATGTTGAGAGTGATACATCTCAAGATGAAGAAGACACTTCTAATCAAGGTGATGAATCCCC
AATTGTGAAATACATCAATAAATTACTAATTGATGCAATTCGCATGGGAGCGTCCGACTTACACTTTGAACCGTATGAGA
AAATGTATCGCGTACGTTATCGTGTTGATGGTGTGCTCAGGCAAATTGCGAATCCACCATTACAAATGGCAAATCGATTG
GCTTCTCGTTTGAAAGTGATGTCTCAGATGGATATTTCTGAGAAGCGCATGCCACAAGATGGCCGAATTAAACTCAAATT
ATCCAAAACAAAAGCCATTGACTTCCGTGTTAACTCTCTTCCAACCTTATTCGGTGAAAAGCTCGTTCTTCGTATTCTTG
ATCCATCGAGCGCGATGCTAGGCATTGAAGCACTCGGCTATGAAGATGAACAGAAAGCATTATTCTTAGAAGCATTAGAA
AAACCGCAAGGTATGCTCCTCATTACTGGCCCTACAGGGTCTGGTAAAACCGTATCTTTATATACTGGTTTAAATATCCT
AAATACAGAAGATACCAATATTTCTACCGCAGAAGATCCTGTTGAGATTAACTTAGAAGGTATTAATCAAGTTAACGTCA
ACCCCAAAACAGGTTTAACCTTTTCTGCTGCGCTAAAAGCATTCTTACGTCAAGATCCTGATATCATCATGGTGGGTGAG
GTTCGTGACCTTGAAACTGCTGAAATTGCAATTAAAGCAGCGCAAACAGGTCATATGGTAATGTCTACTTTACATACAAA
CAGTGCGCCAGAAACACTTACCCGACTACGTAATATGGGAGTACCATCATTCAATATTGCGACCTCTGTCAATTTGGTGA
TTGCTCAGCGCTTAGCACGTCGCCTATGTCAACAGTGTAAAGTACCCGTCGATATCCCTAAACAAAGTCTTATAGAATTA
GGGTTCACCGAACAAGACCTCGCTGATCCTGAATTAAAACTATTTCAGCCAGTAGGTTGTTCAGAATGTAGAGAAGGTTA
CAAGGGCCGTGTTGGTATTTATGAGGTTATGAAAGTAACGCCTGAAATTTCTAAAATTATTATGGAAGATGGTAACGCCC
TACAAATTGCAGCAGCTTCAGAAAAAGCAGGCTTTAACAACTTACGTCGTTCCGGGTTAAAAAAAGTGATGCAAGGAATT
ACTTCTTTACAGGAAGTAAACCGAGTAACTAGTGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-149)

Type II/IV secretion system protein (T2SSE)

(194-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

82.517

100

0.825
  pilB Acinetobacter baylyi ADP1

76.748

100

0.767
  pilB Legionella pneumophila strain ERS1305867

54.444

94.406

0.514
  pilF Neisseria gonorrhoeae MS11

49.912

98.776

0.493
  pilB Vibrio parahaemolyticus RIMD 2210633

49.732

97.727

0.486
  pilB Vibrio campbellii strain DS40M4

49.55

97.028

0.481
  pilB Vibrio cholerae strain A1552

51.252

90.734

0.465
  pilF Thermus thermophilus HB27

37.566

99.126

0.372
  pilB/pilB1 Synechocystis sp. PCC 6803

34.202

100

0.367
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.098

93.007

0.364

Multiple sequence alignment