Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACFELJ_RS24620 Genome accession   NZ_CP172859
Coordinates   5267433..5268653 (+) Length   406 a.a.
NCBI ID   WP_110194327.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 238629E_ID006     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5262433..5273653
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFELJ_RS24600 (ACFELJ_24600) nadC 5264005..5264853 (+) 849 WP_196659286.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACFELJ_RS24610 (ACFELJ_24610) pilA 5265042..5265497 (-) 456 WP_033976389.1 pilin Machinery gene
  ACFELJ_RS24615 (ACFELJ_24615) pilB 5265729..5267429 (+) 1701 WP_033976388.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACFELJ_RS24620 (ACFELJ_24620) pilC 5267433..5268653 (+) 1221 WP_110194327.1 type 4a pilus biogenesis protein PilC Machinery gene
  ACFELJ_RS24625 (ACFELJ_24625) pilD 5268657..5269529 (+) 873 WP_003125204.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  ACFELJ_RS24630 (ACFELJ_24630) coaE 5269526..5270137 (+) 612 WP_033970460.1 dephospho-CoA kinase -
  ACFELJ_RS24635 (ACFELJ_24635) yacG 5270134..5270334 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  ACFELJ_RS24640 (ACFELJ_24640) - 5270371..5270580 (-) 210 WP_003094660.1 hypothetical protein -
  ACFELJ_RS24645 (ACFELJ_24645) - 5270686..5271375 (-) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  ACFELJ_RS24650 (ACFELJ_24650) - 5271372..5271842 (-) 471 WP_003103866.1 hypothetical protein -
  ACFELJ_RS24655 (ACFELJ_24655) - 5271839..5272264 (-) 426 WP_400637226.1 GNAT family N-acetyltransferase -
  ACFELJ_RS24660 (ACFELJ_24660) - 5272397..5273026 (+) 630 WP_003094668.1 DUF1780 domain-containing protein -
  ACFELJ_RS24665 (ACFELJ_24665) - 5273023..5273472 (+) 450 WP_033970456.1 MOSC domain-containing protein -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44360.10 Da        Isoelectric Point: 9.8020

>NTDB_id=1069525 ACFELJ_RS24620 WP_110194327.1 5267433..5268653(+) (pilC) [Pseudomonas aeruginosa strain 238629E_ID006]
MAEKALKTSVFIWEGTDKKGAKVKGELTGQNPMLVKAHLRKQGINPLKVRKKGISLLGAGKKVKPMDIALFTRQMATMMG
AGVPLLQSFDIIGEGFDNPNMRKLVDEIKQEVSSGNSLANSLRKKPQYFDELYCNLVDAGEQSGALENLLDRVATYKEKT
ESLKAKIRKAMTYPIAVIIVALIVSAILLIKVVPQFQSVFQGFGAELPAFTQMVVNLSEFLQEWWLAVIVGVGVIGFTFK
ELHKRSQKFRDTLDRTILKLPIFGGIVYKSAVARYARTLSTTFAAGVPLVDALDSVSGATGNIVFKNAVSKIKQDVSTGM
QLNFSMRTTSVFPNMAIQMTAIGEESGSLDEMLSKVASYYEEEVDNAVDNLTTLMEPMIMAVLGVLVGGLIVAMYLPIFQ
LGNVVG

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=1069525 ACFELJ_RS24620 WP_110194327.1 5267433..5268653(+) (pilC) [Pseudomonas aeruginosa strain 238629E_ID006]
ATGGCGGAAAAAGCGTTAAAAACCAGCGTTTTCATCTGGGAGGGCACCGACAAGAAAGGCGCCAAGGTCAAGGGCGAACT
GACCGGGCAGAATCCCATGCTGGTGAAGGCCCATCTGCGCAAGCAAGGCATCAATCCGCTCAAGGTACGCAAGAAAGGTA
TCTCCCTGCTGGGCGCAGGCAAGAAAGTCAAACCCATGGACATCGCCCTGTTCACCCGGCAGATGGCGACCATGATGGGT
GCTGGCGTTCCCCTCCTGCAATCGTTCGACATCATCGGCGAAGGCTTCGACAATCCCAACATGCGCAAGCTGGTGGATGA
GATCAAGCAAGAAGTGTCCTCGGGCAACAGCCTGGCCAACTCACTGAGAAAGAAACCGCAGTACTTCGACGAACTCTATT
GCAACCTGGTCGATGCCGGCGAGCAGTCGGGCGCCTTGGAAAACTTGCTGGATAGAGTCGCGACCTACAAGGAAAAAACG
GAATCGTTAAAAGCAAAGATCAGGAAGGCGATGACTTACCCTATCGCCGTCATCATCGTTGCCTTGATCGTCTCCGCCAT
TCTGCTGATCAAGGTCGTTCCGCAGTTCCAATCCGTATTCCAAGGTTTTGGCGCAGAGCTACCTGCATTCACTCAAATGG
TCGTAAATTTATCCGAGTTCTTACAGGAGTGGTGGCTTGCCGTCATCGTTGGCGTGGGGGTGATTGGCTTTACCTTCAAG
GAACTGCATAAACGCTCACAAAAGTTCCGCGACACGCTCGACAGAACGATCCTCAAACTTCCCATTTTCGGAGGCATCGT
CTACAAATCCGCGGTAGCCCGTTATGCACGGACCTTGTCCACGACCTTCGCCGCGGGTGTTCCCCTGGTCGATGCGCTCG
ACTCCGTCTCCGGAGCGACCGGCAATATCGTGTTCAAGAACGCGGTCAGCAAGATCAAGCAAGACGTTTCCACCGGCATG
CAGCTCAACTTCTCCATGCGCACCACCAGCGTCTTTCCCAACATGGCGATCCAGATGACCGCCATCGGCGAGGAATCCGG
TTCGCTCGACGAGATGCTGAGCAAAGTCGCCAGCTACTACGAAGAGGAAGTCGACAACGCCGTGGACAACCTCACCACGC
TCATGGAACCGATGATCATGGCCGTTCTCGGCGTACTGGTTGGCGGTCTGATCGTGGCCATGTACCTTCCGATCTTCCAA
CTCGGCAACGTCGTCGGATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

77.037

99.754

0.768
  pilC Acinetobacter baylyi ADP1

60.099

100

0.601
  pilC Acinetobacter baumannii D1279779

60.247

99.754

0.601
  pilC Legionella pneumophila strain ERS1305867

54.545

97.537

0.532
  pilG Neisseria meningitidis 44/76-A

45.658

99.261

0.453
  pilG Neisseria gonorrhoeae MS11

45.409

99.261

0.451
  pilC Vibrio cholerae strain A1552

42.365

100

0.424
  pilC Vibrio campbellii strain DS40M4

41.604

98.276

0.409
  pilC Thermus thermophilus HB27

37.157

98.768

0.367

Multiple sequence alignment