Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACFELK_RS23735 Genome accession   NZ_CP172855
Coordinates   5130933..5132150 (+) Length   405 a.a.
NCBI ID   WP_003107299.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 238633E_ID012     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5125933..5137150
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFELK_RS23710 (ACFELK_23710) nadC 5126055..5126903 (+) 849 WP_003116246.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACFELK_RS23720 (ACFELK_23720) - 5127084..5128442 (-) 1359 WP_225006202.1 O-antigen ligase -
  ACFELK_RS23725 (ACFELK_23725) - 5128534..5128998 (-) 465 WP_003107295.1 pilin -
  ACFELK_RS23730 (ACFELK_23730) pilB 5129229..5130929 (+) 1701 WP_003107297.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACFELK_RS23735 (ACFELK_23735) pilC 5130933..5132150 (+) 1218 WP_003107299.1 type II secretion system F family protein Machinery gene
  ACFELK_RS23740 (ACFELK_23740) pilD 5132151..5133023 (+) 873 WP_003107301.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  ACFELK_RS23745 (ACFELK_23745) coaE 5133020..5133631 (+) 612 WP_003112838.1 dephospho-CoA kinase -
  ACFELK_RS23750 (ACFELK_23750) yacG 5133628..5133828 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  ACFELK_RS23755 (ACFELK_23755) - 5133865..5134074 (-) 210 WP_003094660.1 hypothetical protein -
  ACFELK_RS23760 (ACFELK_23760) - 5134180..5134869 (-) 690 WP_095558192.1 energy-coupling factor ABC transporter permease -
  ACFELK_RS23765 (ACFELK_23765) - 5134866..5135336 (-) 471 WP_003094664.1 hypothetical protein -
  ACFELK_RS23770 (ACFELK_23770) - 5135333..5135758 (-) 426 WP_009875877.1 GNAT family N-acetyltransferase -
  ACFELK_RS23775 (ACFELK_23775) - 5135891..5136520 (+) 630 WP_003094668.1 DUF1780 domain-containing protein -
  ACFELK_RS23780 (ACFELK_23780) - 5136517..5136966 (+) 450 WP_003094670.1 MOSC domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44001.86 Da        Isoelectric Point: 9.8103

>NTDB_id=1069388 ACFELK_RS23735 WP_003107299.1 5130933..5132150(+) (pilC) [Pseudomonas aeruginosa strain 238633E_ID012]
MADKALKTSVFVWEGTDKKGTKVKGELSSQNPTLVKAQLRKQGITPVKVRKKGISLLGAGKKIKPMDIALFTRQMSTMMA
AGVPLLQSFDIISEGFDNPNMRKLVEEIKQEVAGGNSLANSLRKKPQYFDSLYCNLVDAGEQSGALETLLDRVATYKEKT
EALKAKIKKAMTYPIAVIVVAIIVSAILLIKVVPQFQSVFEGFGAELPAFTMMVINISNVLQEWWLLVLLMMGGAGFLLN
HAYKRSEKFRDATDRTVLKLPIVGAILYKSAVARYARTLSTTFAAGVPLVEALDSVSGATGNVVFRDAVGKIKQDVSTGM
QLNFSMRTTNIFPSMAIQMTAIGEESGALDDMLAKVAGFYEQEVDNAVDNLTALMEPMIMAVLGVLVGGLIIAMYLPIFQ
LGNVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=1069388 ACFELK_RS23735 WP_003107299.1 5130933..5132150(+) (pilC) [Pseudomonas aeruginosa strain 238633E_ID012]
ATGGCGGACAAAGCGTTAAAGACCAGTGTCTTCGTATGGGAAGGCACTGACAAGAAAGGCACCAAGGTCAAGGGCGAGCT
GTCCAGCCAGAACCCGACCCTGGTCAAGGCGCAACTGCGCAAGCAGGGCATCACGCCGGTCAAGGTCCGCAAGAAAGGCA
TCTCCCTGCTGGGGGCGGGGAAGAAGATCAAGCCTATGGACATCGCCCTGTTCACCCGCCAGATGTCCACCATGATGGCC
GCCGGCGTGCCGCTGCTGCAGTCGTTCGACATCATCAGCGAAGGCTTCGACAATCCGAACATGCGCAAGCTGGTGGAGGA
GATCAAGCAGGAAGTCGCCGGCGGCAACAGCCTGGCCAACTCGCTGCGCAAGAAGCCACAGTACTTCGACTCGCTCTACT
GCAACCTGGTGGACGCCGGCGAACAATCCGGCGCCCTGGAAACCCTGCTCGACCGGGTCGCCACCTACAAGGAAAAGACC
GAGGCGCTGAAGGCCAAGATCAAGAAGGCCATGACCTACCCGATCGCGGTGATCGTCGTCGCCATCATCGTTTCGGCGAT
CCTACTGATCAAGGTGGTGCCGCAGTTCCAGTCGGTGTTCGAGGGCTTCGGCGCCGAACTGCCAGCCTTCACCATGATGG
TCATCAACATTTCCAACGTACTCCAGGAATGGTGGCTCCTGGTACTGCTGATGATGGGCGGCGCCGGCTTCCTGCTGAAC
CACGCCTACAAGCGTTCGGAGAAGTTCCGCGACGCCACCGACCGCACGGTACTGAAGCTGCCTATCGTCGGCGCCATCCT
CTACAAATCCGCCGTGGCACGCTACGCGCGGACGCTGTCGACCACCTTCGCCGCCGGCGTGCCATTGGTGGAAGCCCTCG
ACTCGGTTTCCGGCGCCACCGGCAACGTGGTGTTCAGGGACGCGGTGGGCAAGATCAAGCAGGACGTGTCCACCGGCATG
CAGCTGAACTTCTCCATGCGCACCACCAACATCTTCCCCAGCATGGCCATCCAGATGACCGCCATCGGCGAGGAGTCGGG
CGCGCTGGACGACATGCTGGCCAAGGTCGCTGGCTTCTACGAGCAGGAAGTGGATAATGCCGTGGACAACCTGACCGCCC
TGATGGAACCGATGATCATGGCGGTCCTCGGGGTCCTGGTCGGTGGCCTGATCATCGCCATGTACCTGCCGATCTTCCAG
CTGGGCAACGTCGTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

78.765

100

0.788
  pilC Acinetobacter baylyi ADP1

61.029

100

0.615
  pilC Acinetobacter baumannii D1279779

60.396

99.753

0.602
  pilC Legionella pneumophila strain ERS1305867

54.293

97.778

0.531
  pilG Neisseria gonorrhoeae MS11

46.04

99.753

0.459
  pilG Neisseria meningitidis 44/76-A

45.522

99.259

0.452
  pilC Vibrio cholerae strain A1552

41.481

100

0.415
  pilC Vibrio campbellii strain DS40M4

40.648

99.012

0.402
  pilC Thermus thermophilus HB27

38.155

99.012

0.378

Multiple sequence alignment