Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACFELI_RS24750 Genome accession   NZ_CP172853
Coordinates   5281666..5282883 (+) Length   405 a.a.
NCBI ID   WP_400523428.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 238635E_ID014     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5276666..5287883
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFELI_RS24725 (ACFELI_24725) nadC 5277312..5278160 (+) 849 WP_017148538.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACFELI_RS24735 (ACFELI_24735) tfpZ 5278394..5279131 (-) 738 WP_023110409.1 TfpX/TfpZ family type IV pilin accessory protein -
  ACFELI_RS24740 (ACFELI_24740) pilE 5279196..5279732 (-) 537 WP_025921208.1 pilin Machinery gene
  ACFELI_RS24745 (ACFELI_24745) pilB 5279959..5281662 (+) 1704 WP_023100230.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACFELI_RS24750 (ACFELI_24750) pilC 5281666..5282883 (+) 1218 WP_400523428.1 type II secretion system F family protein Machinery gene
  ACFELI_RS24755 (ACFELI_24755) pilD 5282886..5283758 (+) 873 WP_017148550.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  ACFELI_RS24760 (ACFELI_24760) coaE 5283755..5284366 (+) 612 WP_400523431.1 dephospho-CoA kinase -
  ACFELI_RS24765 (ACFELI_24765) yacG 5284363..5284563 (+) 201 WP_017148551.1 DNA gyrase inhibitor YacG -
  ACFELI_RS24770 (ACFELI_24770) - 5284600..5284809 (-) 210 WP_003094660.1 hypothetical protein -
  ACFELI_RS24775 (ACFELI_24775) - 5284915..5285604 (-) 690 WP_003094662.1 energy-coupling factor ABC transporter permease -
  ACFELI_RS24780 (ACFELI_24780) - 5285601..5286071 (-) 471 WP_023103507.1 hypothetical protein -
  ACFELI_RS24785 (ACFELI_24785) - 5286068..5286493 (-) 426 WP_016562341.1 GNAT family N-acetyltransferase -
  ACFELI_RS24790 (ACFELI_24790) - 5286626..5287255 (+) 630 WP_003094668.1 DUF1780 domain-containing protein -
  ACFELI_RS24795 (ACFELI_24795) - 5287252..5287701 (+) 450 WP_003161439.1 MOSC domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 43859.86 Da        Isoelectric Point: 9.9457

>NTDB_id=1069318 ACFELI_RS24750 WP_400523428.1 5281666..5282883(+) (pilC) [Pseudomonas aeruginosa strain 238635E_ID014]
MADKALKTSVFIWEGTDKKGSKVKGELTGQNPMLVKAQLRKQGINPLKVRKKGITLFGAGKKIKPMDIALFTRQMATMMG
AGVPLLQSFDIIGEGFENPNMRKLVDEIKQEVAAGNSLANALRKKPQYFDDLYCNLVDAGEQSGALETLLARVATYKEKT
EALKAKIKKAMTYPIAVVIVALVVSAILLIKVVPQFQSVFANFGAELPAFTQMVIGLSETLQEWWLLVLIAMLGIGFALK
ELYKRSKSFRDTIDRAILKAPIVGAIVYKSAVARYARTLSTTFAAGVPLVEALDSVSGATGNVVFKSAVAKIKQDVSSGM
QLNFSMRTTNVFPSMAIQMAAIGEESGSLDDMLGKVAGFYEDEVDNAVDNLTTLMEPMIMAVLGVLVGGLIVAMYLPIFQ
LGKVI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=1069318 ACFELI_RS24750 WP_400523428.1 5281666..5282883(+) (pilC) [Pseudomonas aeruginosa strain 238635E_ID014]
ATGGCGGACAAAGCGTTAAAGACCAGTGTCTTCATCTGGGAAGGTACTGATAAGAAGGGCAGCAAGGTCAAAGGAGAGTT
GACCGGGCAAAACCCTATGCTGGTCAAAGCCCAGCTACGCAAACAGGGTATCAATCCGCTGAAAGTCCGCAAGAAAGGCA
TCACGCTATTCGGTGCGGGAAAAAAAATCAAACCCATGGACATCGCACTGTTCACTCGGCAGATGGCGACCATGATGGGC
GCAGGCGTGCCCCTATTGCAGTCCTTCGACATCATTGGCGAGGGCTTCGAAAATCCGAACATGCGAAAGCTGGTTGACGA
AATAAAGCAGGAAGTCGCAGCCGGTAATAGCTTGGCCAACGCACTACGAAAGAAGCCCCAGTATTTTGACGACCTTTACT
GCAATCTTGTAGATGCGGGCGAACAATCAGGCGCATTGGAAACGCTTCTCGCCCGGGTTGCTACCTACAAGGAAAAAACT
GAGGCACTCAAAGCCAAGATCAAGAAAGCTATGACCTACCCCATCGCGGTCGTTATCGTGGCACTGGTGGTCTCTGCGAT
CCTACTCATTAAGGTTGTTCCGCAATTCCAGTCAGTTTTCGCGAACTTTGGTGCAGAGCTACCAGCCTTCACTCAAATGG
TGATCGGACTTTCGGAAACATTACAAGAATGGTGGCTACTTGTCCTCATCGCCATGCTCGGCATAGGTTTTGCTCTGAAA
GAGTTGTACAAGCGCTCCAAAAGTTTCCGCGACACCATAGACAGAGCCATACTGAAAGCTCCCATCGTCGGTGCCATCGT
CTATAAGTCAGCCGTAGCACGTTATGCACGGACCCTATCGACTACGTTTGCAGCAGGCGTTCCTCTAGTCGAGGCTTTGG
ATTCGGTATCAGGGGCCACAGGTAATGTGGTGTTTAAAAGCGCAGTCGCCAAGATCAAGCAGGACGTTTCCAGCGGTATG
CAGTTGAACTTCTCCATGCGCACCACCAATGTTTTTCCGAGTATGGCTATCCAGATGGCTGCGATTGGCGAAGAATCTGG
TTCGCTGGACGACATGCTTGGCAAAGTGGCTGGCTTCTACGAGGATGAAGTAGATAATGCCGTCGACAATTTGACGACTT
TGATGGAACCAATGATCATGGCGGTGCTGGGAGTCCTGGTTGGCGGATTGATCGTCGCTATGTATCTGCCTATTTTCCAG
CTCGGCAAAGTTATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

79.259

100

0.793
  pilC Acinetobacter baylyi ADP1

59.214

100

0.595
  pilC Acinetobacter baumannii D1279779

59.406

99.753

0.593
  pilC Legionella pneumophila strain ERS1305867

53.283

97.778

0.521
  pilG Neisseria meningitidis 44/76-A

46.173

100

0.462
  pilG Neisseria gonorrhoeae MS11

45.926

100

0.459
  pilC Vibrio cholerae strain A1552

43.21

100

0.432
  pilC Vibrio campbellii strain DS40M4

41.667

97.778

0.407
  pilC Thermus thermophilus HB27

37.656

99.012

0.373

Multiple sequence alignment