Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACAM54_RS25395 Genome accession   NZ_AP028669
Coordinates   5341902..5342363 (-) Length   153 a.a.
NCBI ID   WP_369649323.1    Uniprot ID   -
Organism   Variovorax sp. V93     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5336902..5347363
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACAM54_RS25385 (GmRootV93_50250) - 5338124..5340037 (-) 1914 WP_369649321.1 polysaccharide biosynthesis protein -
  ACAM54_RS25390 (GmRootV93_50260) - 5340089..5341771 (-) 1683 WP_369649322.1 Wzy polymerase domain-containing protein -
  ACAM54_RS25395 (GmRootV93_50270) pilA 5341902..5342363 (-) 462 WP_369649323.1 pilin Machinery gene
  ACAM54_RS25400 (GmRootV93_50280) - 5342567..5343370 (-) 804 WP_369649324.1 3',5'-cyclic-nucleotide phosphodiesterase -
  ACAM54_RS25405 (GmRootV93_50290) - 5343406..5345673 (-) 2268 WP_369649325.1 CHASE2 domain-containing protein -
  ACAM54_RS25410 (GmRootV93_50300) - 5345716..5346369 (-) 654 WP_145739097.1 FHA domain-containing protein -
  ACAM54_RS25415 (GmRootV93_50310) - 5346394..5347194 (-) 801 WP_209500275.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -

Sequence

Protein


Download         Length: 153 a.a.        Molecular weight: 15359.81 Da        Isoelectric Point: 10.1091

>NTDB_id=106835 ACAM54_RS25395 WP_369649323.1 5341902..5342363(-) (pilA) [Variovorax sp. V93]
MNRRSIARNVQKGFTLIELMIVVAIIGILAAVALPAYQDYTVRARVTEAIVIASGAKATIGENAANGASLDSGVDAIASG
SGLKNLSTMSTAAATGVITLVTTSAAGALTLTLTPTANGSALQSGVLPGGPIKWTCAVSSAAKNKFVPTECRI

Nucleotide


Download         Length: 462 bp        

>NTDB_id=106835 ACAM54_RS25395 WP_369649323.1 5341902..5342363(-) (pilA) [Variovorax sp. V93]
ATGAACCGTCGTTCTATCGCACGCAACGTGCAAAAGGGTTTCACCCTTATCGAATTGATGATCGTTGTGGCGATCATCGG
TATCTTGGCTGCTGTGGCTCTGCCGGCGTATCAGGATTACACGGTGCGCGCGCGTGTGACCGAGGCGATTGTCATTGCTT
CTGGCGCGAAGGCGACCATTGGTGAGAATGCCGCGAATGGTGCCTCCCTTGATTCTGGTGTCGACGCCATTGCATCGGGA
TCGGGCCTGAAGAACCTTAGCACCATGAGTACGGCTGCTGCGACCGGCGTCATCACGTTGGTGACAACGTCGGCTGCAGG
TGCTTTGACCCTCACGTTGACCCCGACCGCTAATGGCTCTGCTTTGCAAAGTGGTGTTCTTCCGGGTGGCCCGATCAAGT
GGACGTGCGCAGTTTCCAGTGCTGCAAAGAACAAGTTTGTTCCGACTGAATGCCGCATCTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(8-34)

Pilin (bacterial filament) (Pilin)

(42-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

51.22

100

0.549
  comP Acinetobacter baylyi ADP1

43.671

100

0.451
  pilA2 Legionella pneumophila strain ERS1305867

47.552

93.464

0.444
  pilA2 Legionella pneumophila str. Paris

45.833

94.118

0.431
  pilA/pilA1 Eikenella corrodens VA1

39.873

100

0.412
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40.523

100

0.405
  pilE Neisseria gonorrhoeae strain FA1090

36.747

100

0.399
  pilE Neisseria gonorrhoeae MS11

36.42

100

0.386

Multiple sequence alignment