Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACAM58_RS23930 Genome accession   NZ_AP028661
Coordinates   5015754..5016890 (+) Length   378 a.a.
NCBI ID   WP_015867575.1    Uniprot ID   A0AAW8EJ98
Organism   Variovorax sp. V116     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5010754..5021890
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACAM58_RS23915 (GmRootV116_47470) - 5013452..5013925 (+) 474 WP_018906595.1 Lrp/AsnC ligand binding domain-containing protein -
  ACAM58_RS23920 (GmRootV116_47480) - 5013932..5014630 (-) 699 WP_018906594.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACAM58_RS23925 (GmRootV116_47490) pilT 5014682..5015725 (+) 1044 WP_015867574.1 type IV pilus twitching motility protein PilT Machinery gene
  ACAM58_RS23930 (GmRootV116_47500) pilU 5015754..5016890 (+) 1137 WP_015867575.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACAM58_RS23935 (GmRootV116_47510) - 5016934..5017842 (+) 909 WP_015867576.1 NAD(P)-dependent oxidoreductase -
  ACAM58_RS23940 (GmRootV116_47520) - 5017890..5018666 (-) 777 WP_015867577.1 BON domain-containing protein -
  ACAM58_RS23945 (GmRootV116_47530) - 5018663..5019307 (-) 645 WP_018906593.1 SIS domain-containing protein -
  ACAM58_RS23950 (GmRootV116_47540) - 5019395..5019766 (-) 372 WP_015867579.1 YraN family protein -
  ACAM58_RS23955 (GmRootV116_47550) rsmI 5019812..5020768 (+) 957 WP_018906592.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  ACAM58_RS23960 (GmRootV116_47560) - 5020778..5021617 (-) 840 WP_026284233.1 IclR family transcriptional regulator -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41898.93 Da        Isoelectric Point: 6.4189

>NTDB_id=106745 ACAM58_RS23930 WP_015867575.1 5015754..5016890(+) (pilU) [Variovorax sp. V116]
MERDQASQFINDLLKLMVSRNGSDLFITADFPPAIKVDGKVTKVSQQALGAQHTLALTRSVMNDRQTAEFERTKECNFAI
SPTGIGRFRVNAFVQQGKVGMVLRTIPAKLPTIDGLGMPQILKDVSMTKRGLTILVGATGSGKSTTLAAMIDWRNENSYG
HIVTVEDPVEFVHPHKNCVVTQREVGIDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCMATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRSLISQRLVPTEDGQGRVAAVEVLLNTPLISDLIFKGEVGEIKEIMRKSRNLG
MQTFDQALFDLFESHSITFEDAIRNADSANDLRLQIKLNSQRARSTDLSAGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=106745 ACAM58_RS23930 WP_015867575.1 5015754..5016890(+) (pilU) [Variovorax sp. V116]
ATGGAACGCGATCAAGCCAGCCAGTTCATCAACGACCTGCTCAAGCTCATGGTGAGCCGCAACGGCAGCGACTTGTTCAT
CACCGCGGACTTTCCGCCCGCGATCAAGGTCGACGGCAAGGTCACCAAGGTGTCGCAGCAGGCGCTGGGGGCGCAGCACA
CGCTGGCGCTCACGCGCTCGGTCATGAACGACCGCCAGACGGCGGAGTTCGAGCGCACCAAGGAATGCAACTTCGCGATC
TCGCCGACCGGCATCGGCCGCTTCCGCGTGAACGCCTTCGTGCAGCAGGGCAAGGTCGGCATGGTGCTGCGGACCATTCC
GGCCAAGCTGCCGACCATCGACGGCCTGGGCATGCCGCAGATCCTGAAGGACGTGTCGATGACCAAGCGCGGCCTCACCA
TCCTGGTGGGCGCCACGGGCTCGGGCAAGTCGACCACGCTGGCGGCCATGATCGACTGGCGCAACGAAAATTCCTACGGC
CACATCGTGACGGTGGAGGACCCGGTCGAATTCGTGCATCCGCACAAGAACTGCGTGGTCACGCAGCGCGAAGTGGGCAT
CGACACCGACAGCTGGGAAGCCGCGCTCAAGAACACGCTGCGCCAGGCGCCCGACGTGATCCTGATGGGCGAAATCCGCG
ACCGCGAGACCATGGAGCACGCCGTGGCCTTTGCCGAGACCGGCCACCTGTGCATGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTGGACCGGATCATCAACTTCTTCCCCGAAGAGCGTCGCGCGCAACTGTTGATGGACCTGTCGCTGAACCT
GCGCTCGCTGATCTCCCAGCGCCTGGTGCCCACCGAGGACGGCCAGGGCCGCGTGGCGGCGGTGGAAGTGCTGCTGAACA
CGCCGCTCATTTCCGACCTGATCTTCAAGGGCGAGGTGGGCGAGATCAAGGAGATCATGCGCAAAAGCCGCAACCTCGGC
ATGCAGACCTTCGACCAGGCGCTGTTCGACCTGTTCGAAAGCCACTCGATCACCTTCGAGGACGCCATCCGCAACGCCGA
TTCGGCCAACGACCTGCGGCTGCAGATCAAGCTCAACAGCCAGCGCGCGCGCAGCACCGACCTCTCGGCCGGCACCGAGC
ACTTCGCGATCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(48-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.61

92.328

0.587
  pilU Acinetobacter baylyi ADP1

58.101

94.709

0.55
  pilU Vibrio cholerae strain A1552

52.893

96.032

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.994

89.153

0.41
  pilT Pseudomonas aeruginosa PAK

44.807

89.153

0.399
  pilT Acinetobacter nosocomialis M2

44.144

88.095

0.389
  pilT Pseudomonas stutzeri DSM 10701

42.73

89.153

0.381
  pilT Acinetobacter baumannii strain A118

45.141

84.392

0.381
  pilT Acinetobacter baumannii D1279779

45.141

84.392

0.381
  pilT Acinetobacter baylyi ADP1

42.943

88.095

0.378
  pilT Legionella pneumophila strain Lp02

43.465

87.037

0.378
  pilT Legionella pneumophila strain ERS1305867

43.465

87.037

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.218

83.862

0.362
  pilT Vibrio cholerae strain A1552

43.218

83.862

0.362

Multiple sequence alignment