Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACF0KO_RS19900 Genome accession   NZ_CP172307
Coordinates   4539998..4541158 (-) Length   386 a.a.
NCBI ID   WP_064030925.1    Uniprot ID   A0A177NBY0
Organism   Methylomonas sp. 2B     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4534998..4546158
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACF0KO_RS19870 (ACF0KO_19870) lexA 4536361..4536987 (+) 627 WP_064030921.1 transcriptional repressor LexA -
  ACF0KO_RS19875 (ACF0KO_19875) - 4537002..4537151 (+) 150 WP_157197959.1 hypothetical protein -
  ACF0KO_RS19880 (ACF0KO_19880) - 4537174..4537506 (+) 333 WP_064030922.1 hypothetical protein -
  ACF0KO_RS19885 (ACF0KO_19885) - 4538139..4538678 (-) 540 WP_064030923.1 hypothetical protein -
  ACF0KO_RS19890 (ACF0KO_19890) - 4538787..4539629 (-) 843 WP_064030924.1 endonuclease/exonuclease/phosphatase family protein -
  ACF0KO_RS19895 (ACF0KO_19895) - 4539660..4539872 (-) 213 WP_064030962.1 DUF6447 family protein -
  ACF0KO_RS19900 (ACF0KO_19900) pilU 4539998..4541158 (-) 1161 WP_064030925.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACF0KO_RS19905 (ACF0KO_19905) - 4541277..4542320 (+) 1044 WP_064030926.1 tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase -
  ACF0KO_RS19910 (ACF0KO_19910) - 4542317..4542541 (+) 225 WP_064030927.1 sulfurtransferase TusA family protein -
  ACF0KO_RS19915 (ACF0KO_19915) argJ 4542587..4543801 (+) 1215 WP_064030928.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -
  ACF0KO_RS19920 (ACF0KO_19920) - 4543803..4544759 (+) 957 WP_064030929.1 Nudix family hydrolase -
  ACF0KO_RS19925 (ACF0KO_19925) yacG 4544744..4544947 (-) 204 WP_064030930.1 DNA gyrase inhibitor YacG -
  ACF0KO_RS19930 (ACF0KO_19930) zapD 4544947..4545717 (-) 771 WP_082885650.1 cell division protein ZapD -

Sequence

Protein


Download         Length: 386 a.a.        Molecular weight: 42662.68 Da        Isoelectric Point: 5.6124

>NTDB_id=1066203 ACF0KO_RS19900 WP_064030925.1 4539998..4541158(-) (pilU) [Methylomonas sp. 2B]
MEFKDYLKILVQQDGSDLYLTAGAPPAAKFHGTLRPLENIRLTNERLKEIAHAIMDADQRAAFEQVPEMNLAIAEEGVGR
FRVNIFKQRSNYALVIRNIKVDIPNADTLGLPQVLKDKIMEKRGLILFVGGTGSGKSTSLAALIDYRNSHASGHIITIED
PIEFVHPHKKSLVNQREVGVDTLSYEDALKNTLRQAPDVILIGEIRSQETMEHALAFAETGHLCLSTLHANNANQALDRI
INFFPEERRPQLLMDLSLNVQAFVSQRLVPTVEGKRVAAIEILLGSKLVSDLIQKGDVHAIKEAMEKSENIGMQTFDSHL
LKLYKSGVISLDEALRNSDSPNNLKLKINLSEGLGSATGDDKPSLADTLSLQEIVKPEENEDEGGH

Nucleotide


Download         Length: 1161 bp        

>NTDB_id=1066203 ACF0KO_RS19900 WP_064030925.1 4539998..4541158(-) (pilU) [Methylomonas sp. 2B]
ATGGAATTTAAAGATTACCTGAAAATTTTGGTTCAACAAGACGGTTCGGATTTATATCTGACCGCAGGCGCGCCGCCGGC
TGCTAAATTTCACGGCACGCTGCGGCCGCTGGAAAACATTCGACTGACCAACGAAAGACTTAAAGAGATAGCCCATGCCA
TCATGGATGCCGATCAGCGCGCGGCCTTCGAACAGGTACCGGAAATGAATCTAGCCATCGCCGAGGAAGGCGTGGGCCGG
TTTAGGGTGAATATTTTCAAGCAACGCAGCAATTACGCGCTGGTGATCCGCAACATCAAGGTCGATATTCCCAACGCCGA
CACCCTGGGCTTGCCGCAGGTGCTGAAAGACAAAATTATGGAAAAGCGCGGCCTGATCTTGTTCGTCGGCGGCACCGGCT
CCGGCAAATCCACCTCGCTGGCGGCCTTGATCGATTACCGCAACAGCCATGCGTCCGGCCACATCATTACCATCGAAGAT
CCGATCGAGTTCGTCCATCCGCACAAGAAATCGCTGGTGAATCAGCGCGAGGTCGGCGTCGATACGCTCAGCTATGAAGA
CGCGCTGAAAAACACGCTGCGCCAAGCGCCCGACGTGATTTTGATCGGCGAGATACGCAGTCAGGAGACGATGGAGCACG
CGCTGGCCTTCGCCGAAACCGGCCATCTGTGTCTGTCCACCTTGCATGCCAATAACGCTAATCAGGCCTTGGACCGGATC
ATCAATTTCTTTCCGGAAGAACGCCGTCCGCAATTGTTGATGGATTTGTCGCTGAACGTGCAGGCCTTTGTGTCGCAACG
CTTGGTGCCGACCGTGGAAGGCAAGCGGGTGGCGGCGATCGAGATTTTGTTGGGCAGCAAACTGGTCAGCGATTTGATTC
AGAAAGGCGATGTGCACGCGATCAAGGAAGCGATGGAAAAATCGGAAAACATCGGCATGCAAACCTTCGACAGCCATTTG
CTCAAGCTCTACAAAAGCGGCGTGATTTCGCTGGACGAAGCATTGCGCAACTCCGACTCGCCGAACAATTTGAAACTCAA
AATCAATCTCAGCGAAGGCCTGGGTTCGGCGACCGGCGACGACAAACCCAGCCTGGCCGATACCTTGTCGTTGCAGGAAA
TCGTCAAGCCTGAGGAAAATGAAGACGAGGGCGGGCATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(68-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A177NBY0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

54.521

97.409

0.531
  pilU Acinetobacter baylyi ADP1

52.825

91.71

0.484
  pilU Vibrio cholerae strain A1552

50

93.264

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.389

93.264

0.386
  pilT Pseudomonas aeruginosa PAK

40.698

89.119

0.363
  pilT Pseudomonas stutzeri DSM 10701

40.698

89.119

0.363
  pilT Legionella pneumophila strain ERS1305867

42.121

85.492

0.36
  pilT Legionella pneumophila strain Lp02

42.121

85.492

0.36

Multiple sequence alignment