Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACF0H7_RS03155 Genome accession   NZ_CP172305
Coordinates   641531..642748 (-) Length   405 a.a.
NCBI ID   WP_397451357.1    Uniprot ID   -
Organism   Pseudomonas sp. NA-150     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 636531..647748
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACF0H7_RS03130 (ACF0H7_03130) - 638068..638898 (-) 831 WP_397451352.1 type 1 glutamine amidotransferase domain-containing protein -
  ACF0H7_RS03135 (ACF0H7_03135) - 639112..639810 (+) 699 WP_397451353.1 energy-coupling factor ABC transporter permease -
  ACF0H7_RS03140 (ACF0H7_03140) yacG 639836..640039 (-) 204 WP_397451354.1 DNA gyrase inhibitor YacG -
  ACF0H7_RS03145 (ACF0H7_03145) coaE 640036..640659 (-) 624 WP_397451355.1 dephospho-CoA kinase -
  ACF0H7_RS03150 (ACF0H7_03150) pilD 640656..641528 (-) 873 WP_397451356.1 prepilin peptidase Machinery gene
  ACF0H7_RS03155 (ACF0H7_03155) pilC 641531..642748 (-) 1218 WP_397451357.1 type II secretion system F family protein Machinery gene
  ACF0H7_RS03160 (ACF0H7_03160) pilB 642751..644310 (-) 1560 Protein_606 type IV-A pilus assembly ATPase PilB -
  ACF0H7_RS03165 (ACF0H7_03165) - 644300..644521 (-) 222 Protein_607 IS21 family transposase -
  ACF0H7_RS03170 (ACF0H7_03170) - 644599..644877 (+) 279 Protein_608 aldehyde dehydrogenase -
  ACF0H7_RS03175 (ACF0H7_03175) - 645022..645408 (-) 387 WP_397451358.1 response regulator -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44137.95 Da        Isoelectric Point: 9.9934

>NTDB_id=1066131 ACF0H7_RS03155 WP_397451357.1 641531..642748(-) (pilC) [Pseudomonas sp. NA-150]
MATKAVKVSVYAWEGTDKKGAKLSGELNGHNQALVKAQLRKQGINPIKVRKKAISIFGEGKKIKPLDIAFFTRQMATMMK
AGVPLLQSFDIIGESSDNPRMRTLVNEIKQEVAAGNSFANSLKKKPEYFDELYCSLVDAGEQAGALETLLDRVATYKEKT
EKLKAKIKKAMSYPIAVVVVALIVSAILLIKVVPQFQAVFSSFGAKLPAFTLMVIGLSEILQNWWYIVLAGFVVATFGFK
RAYKTSQKFRDSLDRGLLKVPVVGPLIYKSSVARYARTLSTTFAAGVPLVEALDSVAGATGNVVFKNAVIRIKQDVSTGM
QLNFSMRTTGVFPTLAIQMTAIGEESGALDSMLDKVATYYEDEVDDMVDNMTALMEPFIMAVLGVVVGGLVIAMYLPIFQ
LGNVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=1066131 ACF0H7_RS03155 WP_397451357.1 641531..642748(-) (pilC) [Pseudomonas sp. NA-150]
ATGGCGACCAAAGCAGTCAAAGTCAGTGTTTACGCCTGGGAAGGTACTGACAAGAAAGGCGCCAAACTCAGCGGTGAGTT
GAATGGGCATAACCAGGCGCTGGTCAAAGCCCAATTGCGCAAGCAAGGCATCAATCCGATCAAGGTGCGCAAGAAAGCAA
TCTCGATTTTCGGCGAAGGCAAGAAAATCAAACCGCTGGACATCGCTTTTTTTACTCGCCAAATGGCAACGATGATGAAA
GCGGGGGTTCCATTACTGCAGTCCTTCGACATCATCGGAGAAAGCTCCGACAACCCGCGGATGCGCACTTTGGTCAATGA
GATCAAACAAGAAGTGGCTGCCGGTAACAGTTTCGCTAATTCCTTGAAGAAGAAGCCAGAGTACTTCGACGAGTTGTACT
GCAGCCTAGTTGATGCTGGGGAGCAAGCAGGCGCACTCGAAACCCTGCTAGATCGGGTAGCTACTTATAAAGAAAAAACA
GAAAAGCTCAAAGCCAAAATCAAGAAAGCAATGTCCTACCCTATTGCGGTGGTGGTCGTAGCCTTGATTGTCTCAGCCAT
TTTGTTGATCAAAGTCGTGCCTCAATTTCAGGCGGTTTTTTCCAGCTTTGGCGCAAAGCTACCAGCCTTCACACTGATGG
TTATAGGTCTTTCCGAAATATTGCAAAACTGGTGGTATATCGTTTTGGCGGGATTTGTAGTAGCGACGTTCGGCTTTAAA
CGCGCATATAAGACCTCCCAAAAATTTCGCGACTCATTAGACCGTGGACTACTCAAAGTCCCCGTCGTCGGTCCATTAAT
CTACAAATCATCCGTAGCCCGCTATGCCCGTACCCTCTCCACAACTTTTGCGGCCGGCGTACCCCTGGTAGAAGCACTGG
ATTCCGTAGCCGGAGCAACCGGTAACGTCGTATTCAAAAACGCGGTGATCAGAATCAAACAAGACGTTTCCACCGGCATG
CAACTGAACTTCTCCATGCGCACCACCGGCGTGTTCCCAACCCTAGCCATCCAGATGACAGCCATCGGCGAAGAATCCGG
TGCCCTCGACTCCATGCTCGACAAAGTCGCCACCTACTACGAAGACGAAGTCGACGATATGGTCGATAACATGACCGCGC
TGATGGAGCCCTTCATCATGGCCGTTCTCGGTGTCGTGGTCGGCGGCCTCGTTATTGCCATGTACCTACCCATCTTCCAG
CTCGGAAACGTCGTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

78.025

100

0.78
  pilC Acinetobacter baylyi ADP1

60.391

100

0.61
  pilC Acinetobacter baumannii D1279779

59.259

100

0.593
  pilC Legionella pneumophila strain ERS1305867

52.02

97.778

0.509
  pilG Neisseria gonorrhoeae MS11

41.791

99.259

0.415
  pilC Vibrio campbellii strain DS40M4

41.133

100

0.412
  pilG Neisseria meningitidis 44/76-A

41.542

99.259

0.412
  pilC Vibrio cholerae strain A1552

40.741

100

0.407
  pilC Thermus thermophilus HB27

36.409

99.012

0.36

Multiple sequence alignment