Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACGB44_RS05330 Genome accession   NZ_CP171478
Coordinates   1143615..1144832 (+) Length   405 a.a.
NCBI ID   WP_394375548.1    Uniprot ID   -
Organism   Stutzerimonas frequens strain RL-XB02     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1138615..1149832
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACGB44_RS05315 (ACGB44_05315) cysN 1138679..1140577 (+) 1899 WP_394375545.1 sulfate adenylyltransferase subunit CysN -
  ACGB44_RS05320 (ACGB44_05320) - 1141160..1141600 (-) 441 WP_394375546.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ACGB44_RS05325 (ACGB44_05325) pilB 1141910..1143613 (+) 1704 WP_394375547.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACGB44_RS05330 (ACGB44_05330) pilC 1143615..1144832 (+) 1218 WP_394375548.1 type II secretion system F family protein Machinery gene
  ACGB44_RS05335 (ACGB44_05335) - 1144835..1145705 (+) 871 Protein_1052 prepilin peptidase -
  ACGB44_RS05340 (ACGB44_05340) coaE 1145838..1146446 (+) 609 WP_316959140.1 dephospho-CoA kinase -
  ACGB44_RS05345 (ACGB44_05345) yacG 1146443..1146640 (+) 198 WP_102840588.1 DNA gyrase inhibitor YacG -
  ACGB44_RS05350 (ACGB44_05350) - 1146665..1147345 (-) 681 WP_316959141.1 energy-coupling factor ABC transporter permease -
  ACGB44_RS05355 (ACGB44_05355) - 1147384..1148421 (-) 1038 WP_316959142.1 NADP(H)-dependent aldo-keto reductase -
  ACGB44_RS05360 (ACGB44_05360) rplM 1148865..1149293 (+) 429 WP_063544471.1 50S ribosomal protein L13 -
  ACGB44_RS05365 (ACGB44_05365) rpsI 1149308..1149700 (+) 393 WP_013981948.1 30S ribosomal protein S9 -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44119.98 Da        Isoelectric Point: 9.8752

>NTDB_id=1061390 ACGB44_RS05330 WP_394375548.1 1143615..1144832(+) (pilC) [Stutzerimonas frequens strain RL-XB02]
MAQKAIKTSVFTWEGTNKQGAKIKGELSGISPAMVKAQLRKQGVNPQKVRKKSASLFSAGKKIKPMDIALFTRQMATMMK
AGVPLLQSFDIIGEGFDNPNMRKLVDDLKQEVAAGNSFATALRKKPQYFDDLYCNLVDSGEQSGSLEALLDRVATYKEKT
EALKAKIKKAMNYPIAVVLVAIIVSAILLIKVVPQFQEVFANFGAELPAFTLMVIGLSEILQAWWHLVLLGLFLAAFAFK
TAHGKSERFRDWFDRLLLKMPIVGEILYKSAVARFARTLSTTFAAGVPLVDALDSVAGATGNVVFRNATMKVKSDVSSGM
QLNFSMRTTGTFPTMAVQMAAIGEESGSLDEMLAKVATFYEDEVDNMVDGLTSLMEPMIMAVLGVLVGGLIIAMYLPIFQ
LGSVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=1061390 ACGB44_RS05330 WP_394375548.1 1143615..1144832(+) (pilC) [Stutzerimonas frequens strain RL-XB02]
ATGGCGCAGAAAGCGATTAAAACCAGTGTGTTCACCTGGGAAGGCACCAACAAGCAGGGTGCGAAGATCAAGGGCGAGCT
AAGCGGGATCAGCCCTGCGATGGTCAAGGCGCAGCTGCGTAAGCAGGGCGTAAACCCTCAAAAGGTGCGTAAGAAGTCGG
CGTCGCTGTTCAGTGCTGGCAAGAAGATCAAGCCGATGGATATCGCCTTGTTCACTCGTCAGATGGCGACCATGATGAAG
GCAGGTGTGCCGCTGCTGCAGTCATTCGACATTATTGGCGAGGGGTTCGATAACCCGAACATGCGTAAGCTGGTCGATGA
TCTCAAGCAGGAGGTCGCGGCGGGTAACAGTTTCGCCACTGCGTTGCGCAAGAAGCCGCAGTACTTTGACGATCTCTACT
GCAACCTGGTGGACTCGGGTGAGCAGTCTGGTTCCTTGGAGGCGTTGCTCGATCGCGTTGCAACCTACAAGGAGAAAACC
GAAGCTCTCAAGGCAAAGATCAAGAAGGCGATGAATTATCCGATCGCTGTCGTACTCGTCGCGATCATTGTTTCCGCGAT
TTTGTTGATCAAGGTCGTTCCTCAGTTTCAGGAAGTTTTTGCCAACTTCGGCGCGGAGCTTCCAGCGTTTACGCTCATGG
TCATTGGTCTGTCCGAAATCCTGCAGGCCTGGTGGCACCTGGTCCTGCTGGGGTTGTTCCTCGCTGCCTTCGCATTCAAG
ACCGCCCACGGTAAATCGGAGCGGTTTCGCGACTGGTTTGACCGCTTGCTGCTGAAGATGCCGATCGTCGGCGAGATTCT
TTACAAATCGGCAGTTGCACGATTCGCTCGGACCTTGTCCACGACGTTCGCTGCGGGTGTGCCTTTGGTGGACGCTCTGG
ATTCGGTTGCCGGGGCAACCGGTAATGTGGTGTTCCGCAATGCAACGATGAAAGTCAAAAGTGACGTATCGTCTGGCATG
CAGCTGAACTTCTCCATGCGTACCACCGGTACATTTCCGACCATGGCGGTACAGATGGCGGCGATTGGCGAGGAGTCAGG
CTCGCTTGACGAGATGCTGGCCAAGGTGGCGACCTTCTACGAGGATGAGGTCGACAACATGGTCGATGGTCTGACAAGCC
TGATGGAGCCGATGATCATGGCTGTTCTCGGGGTCCTGGTTGGTGGTTTGATCATTGCCATGTATCTCCCGATTTTCCAA
CTGGGTTCCGTCGTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

89.877

100

0.899
  pilC Acinetobacter baylyi ADP1

59.36

100

0.595
  pilC Acinetobacter baumannii D1279779

58.025

100

0.58
  pilC Legionella pneumophila strain ERS1305867

53.283

97.778

0.521
  pilG Neisseria gonorrhoeae MS11

45.161

99.506

0.449
  pilG Neisseria meningitidis 44/76-A

45.161

99.506

0.449
  pilC Vibrio cholerae strain A1552

43.434

97.778

0.425
  pilC Vibrio campbellii strain DS40M4

41.414

97.778

0.405

Multiple sequence alignment