Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACGB44_RS05325 Genome accession   NZ_CP171478
Coordinates   1141910..1143613 (+) Length   567 a.a.
NCBI ID   WP_394375547.1    Uniprot ID   -
Organism   Stutzerimonas frequens strain RL-XB02     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1136910..1148613
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACGB44_RS05310 (ACGB44_05310) cysD 1137749..1138666 (+) 918 WP_110777989.1 sulfate adenylyltransferase subunit CysD -
  ACGB44_RS05315 (ACGB44_05315) cysN 1138679..1140577 (+) 1899 WP_394375545.1 sulfate adenylyltransferase subunit CysN -
  ACGB44_RS05320 (ACGB44_05320) - 1141160..1141600 (-) 441 WP_394375546.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ACGB44_RS05325 (ACGB44_05325) pilB 1141910..1143613 (+) 1704 WP_394375547.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACGB44_RS05330 (ACGB44_05330) pilC 1143615..1144832 (+) 1218 WP_394375548.1 type II secretion system F family protein Machinery gene
  ACGB44_RS05335 (ACGB44_05335) - 1144835..1145705 (+) 871 Protein_1052 prepilin peptidase -
  ACGB44_RS05340 (ACGB44_05340) coaE 1145838..1146446 (+) 609 WP_316959140.1 dephospho-CoA kinase -
  ACGB44_RS05345 (ACGB44_05345) yacG 1146443..1146640 (+) 198 WP_102840588.1 DNA gyrase inhibitor YacG -
  ACGB44_RS05350 (ACGB44_05350) - 1146665..1147345 (-) 681 WP_316959141.1 energy-coupling factor ABC transporter permease -
  ACGB44_RS05355 (ACGB44_05355) - 1147384..1148421 (-) 1038 WP_316959142.1 NADP(H)-dependent aldo-keto reductase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 61856.82 Da        Isoelectric Point: 5.2370

>NTDB_id=1061389 ACGB44_RS05325 WP_394375547.1 1141910..1143613(+) (pilB) [Stutzerimonas frequens strain RL-XB02]
MSENTSLSGLARQMVAAGVLDEKTAQQAQAQAIRGKTPFVTYVVQNRLAKGRVIAELASEQFGVALLDLAALDKESLPKD
LVSEKLAKQHRVLPLSKRGTKLYVAVSDPANHQSIADIQFSTGLSIEPILVEDDKLGEAIDKLYDSGATGLEDLGEVDLD
GLDVESVSDDGRADDAGEVADDAPVVRFVNKMLLDAIRGGSSDLHFEPYERAYRVRFRTDGILHEIARPPIQLAPRISAR
LKVMAGLDISERRKPQDGRIKMKLSKTKAIDFRVNTLPTLWGEKIVMRILDPSSAQMGIDALGYEEEQKALYMQALQQPQ
GMILVTGPTGSGKTVSLYTGLNILNTVDVNISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDIIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVAAFNIATSVNLIIAQRLARKLCNSCKKEIDLPREALLEEGFP
ADKIGAFKIYGPVGCDNCKGGYKGRVGIYEVVKNTPALARIIMEDGNSIDISTQMRKDGFNDLRTSALLKAMQGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=1061389 ACGB44_RS05325 WP_394375547.1 1141910..1143613(+) (pilB) [Stutzerimonas frequens strain RL-XB02]
ATGAGCGAGAATACTTCCCTTTCCGGGCTGGCGCGGCAGATGGTTGCGGCGGGTGTGCTGGACGAGAAGACGGCGCAGCA
GGCCCAGGCTCAGGCCATTCGGGGCAAGACACCCTTTGTCACCTACGTCGTCCAGAACCGGTTGGCCAAGGGCCGCGTCA
TTGCCGAGCTCGCCTCCGAGCAGTTCGGTGTTGCGCTGCTGGATCTTGCTGCGTTGGATAAGGAGTCGCTGCCCAAGGAT
CTGGTGAGCGAGAAGCTGGCCAAGCAGCACCGGGTCCTTCCCCTCTCCAAGCGCGGCACCAAGCTGTATGTGGCGGTGTC
CGACCCTGCCAACCATCAATCGATTGCCGATATCCAGTTCAGCACCGGGCTTTCGATCGAGCCGATTCTGGTCGAGGACG
ACAAACTCGGGGAGGCGATCGACAAGCTGTACGATAGCGGTGCAACCGGCCTGGAAGATCTGGGAGAGGTCGATCTCGAT
GGGCTGGATGTCGAGTCCGTTAGCGACGACGGCCGGGCGGATGATGCGGGGGAGGTCGCGGATGATGCGCCGGTCGTGCG
CTTCGTCAACAAGATGTTACTCGATGCCATTCGTGGTGGTTCTTCCGACTTGCACTTCGAGCCCTATGAAAGAGCCTATC
GAGTCCGGTTCCGCACCGATGGGATTCTGCACGAGATCGCTCGCCCCCCGATCCAGTTGGCGCCTCGCATATCGGCCCGT
CTGAAAGTGATGGCAGGGTTGGATATATCCGAGCGGCGTAAACCCCAGGACGGTCGGATCAAGATGAAGCTGTCCAAGAC
CAAGGCGATCGATTTTCGCGTCAATACCTTGCCTACGCTCTGGGGCGAAAAGATCGTGATGCGAATCCTCGACCCGTCCA
GTGCGCAGATGGGGATCGATGCGCTGGGGTACGAGGAAGAACAGAAAGCGCTCTATATGCAGGCGCTTCAGCAGCCACAG
GGCATGATCCTGGTAACCGGTCCTACGGGTTCGGGCAAAACGGTATCCCTTTATACCGGTCTCAATATCCTCAATACGGT
CGACGTAAATATCTCCACCGCTGAGGATCCGGTAGAAATCAACCTGGAAGGTATCAATCAGGTCAACGTCAATCCCCGTC
AGGGGATGGACTTCTCCCAGGCGCTGCGCGCATTTCTGCGGCAGGACCCCGATATCATCATGGTGGGCGAGATTCGGGAT
CTGGAAACTGCCGAGATCGCCATCAAGGCCGCGCAGACAGGCCATATGGTAATGTCGACGCTGCACACCAACAGCGCCGC
AGAAACCCTTACACGCCTTCGCAACATGGGGGTGGCAGCGTTCAATATCGCTACGTCGGTGAACCTGATCATTGCTCAGC
GACTGGCGCGCAAGCTATGTAACAGTTGCAAGAAAGAGATCGATCTGCCGCGCGAGGCTCTGTTGGAAGAGGGCTTCCCC
GCCGACAAGATTGGAGCCTTCAAGATCTATGGTCCGGTTGGTTGCGATAATTGCAAAGGCGGTTACAAAGGTCGTGTCGG
TATTTATGAAGTGGTTAAAAACACGCCGGCGTTGGCTAGGATTATCATGGAAGACGGTAATTCGATCGATATTTCCACCC
AGATGCGAAAAGACGGCTTCAATGACCTGCGCACCTCGGCTCTGCTCAAGGCCATGCAGGGCGTGACCAGCCTGGAAGAA
GTCAACCGGGTGACCAAGGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(15-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.929

99.471

0.596
  pilB Acinetobacter baylyi ADP1

59.68

99.295

0.593
  pilB Legionella pneumophila strain ERS1305867

55.769

100

0.563
  pilB Vibrio campbellii strain DS40M4

48.258

100

0.489
  pilF Neisseria gonorrhoeae MS11

48.49

99.295

0.481
  pilB Vibrio cholerae strain A1552

53.216

90.476

0.481
  pilB Vibrio parahaemolyticus RIMD 2210633

51.644

91.182

0.471

Multiple sequence alignment