Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACGI6K_RS16430 Genome accession   NZ_CP171449
Coordinates   3407001..3408701 (+) Length   566 a.a.
NCBI ID   WP_376943561.1    Uniprot ID   -
Organism   Azorhizophilus paspali strain ATCC 23833     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3402001..3413701
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACGI6K_RS16405 (ACGI6K_16405) - 3402031..3402810 (+) 780 WP_376943544.1 TatD family hydrolase -
  ACGI6K_RS16410 (ACGI6K_16410) ampE 3402951..3403769 (-) 819 WP_376943542.1 regulatory signaling modulator protein AmpE -
  ACGI6K_RS16415 (ACGI6K_16415) ampD 3403766..3404329 (-) 564 WP_376943540.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACGI6K_RS16420 (ACGI6K_16420) nadC 3404472..3405320 (+) 849 WP_376943538.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACGI6K_RS16425 (ACGI6K_16425) - 3405977..3406474 (-) 498 WP_376943536.1 pilin -
  ACGI6K_RS16430 (ACGI6K_16430) pilB 3407001..3408701 (+) 1701 WP_376943561.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACGI6K_RS16435 (ACGI6K_16435) pilC 3408926..3410140 (+) 1215 WP_376943559.1 type II secretion system F family protein Machinery gene
  ACGI6K_RS16440 (ACGI6K_16440) pilD 3410143..3411012 (+) 870 WP_376943534.1 prepilin peptidase Machinery gene
  ACGI6K_RS16445 (ACGI6K_16445) coaE 3411175..3411783 (+) 609 WP_376943532.1 dephospho-CoA kinase -
  ACGI6K_RS16450 (ACGI6K_16450) yacG 3411780..3411980 (+) 201 WP_041806982.1 DNA gyrase inhibitor YacG -
  ACGI6K_RS16455 (ACGI6K_16455) - 3412079..3412765 (-) 687 WP_376943530.1 energy-coupling factor ABC transporter permease -
  ACGI6K_RS16460 (ACGI6K_16460) - 3412762..3413229 (-) 468 WP_376943528.1 FAD/FMN-containing dehydrogenase -
  ACGI6K_RS16465 (ACGI6K_16465) - 3413226..3413651 (-) 426 WP_376943526.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62576.66 Da        Isoelectric Point: 6.4463

>NTDB_id=1061256 ACGI6K_RS16430 WP_376943561.1 3407001..3408701(+) (pilB) [Azorhizophilus paspali strain ATCC 23833]
MHDDISLHGLARQMVLAGLIDEKTVLQAQRQALRNQTPLVTWLVQNKLVKSRGLAELAAEQFGIALFDLGTLERENQPRD
LLSEKLIRQHRALPLWRRGNRLFVAISDPTNHEAIREIRFGTGLNTEAILVEDDRLGEAMEKYFEGTGSALDDLTDAGPD
GLDIEAGDRHDEALNPAGDAEDAPVVRFINKILLDAIRRGSSDLHFEPYEKSHRVRFRTDGILHEVARPPVRSAPKIAAR
LKVMAGLDISERRKPQDGRIRMKLPKGNAIDFRVNTLPTLWGEKVVMRILDPSSAQMGIDALGYEESQKALYLEALTQPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTVEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDIIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPSFNIATSVNLIIAQRLARKLCACKQAVDIPHETLLAEGFPE
KRIGVFRLYAPTGCENCNGGYKGRVGIYEVVKITPALQRIIMGDGNSIDIARQMRAEGFNDLRASALQKAMQGVTSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=1061256 ACGI6K_RS16430 WP_376943561.1 3407001..3408701(+) (pilB) [Azorhizophilus paspali strain ATCC 23833]
ATGCACGACGACATTTCCCTCCACGGTCTGGCACGGCAGATGGTGCTGGCCGGACTGATCGACGAGAAGACGGTCCTGCA
GGCCCAGCGGCAGGCGCTGCGCAACCAAACCCCGCTGGTCACCTGGCTAGTGCAGAACAAGCTGGTCAAGAGCCGGGGAT
TGGCGGAGCTGGCCGCGGAGCAGTTCGGCATCGCCTTGTTCGATCTCGGGACGCTGGAGCGGGAAAACCAGCCCCGCGAC
TTGCTCAGCGAGAAACTAATCCGCCAGCATCGCGCTCTGCCGTTGTGGCGGCGCGGCAACCGGCTGTTCGTGGCGATTTC
CGACCCGACCAACCACGAGGCGATTCGCGAGATCCGCTTCGGCACCGGGCTGAACACCGAAGCCATCCTGGTCGAGGACG
ACCGTCTTGGCGAAGCCATGGAGAAGTACTTCGAGGGCACCGGTAGCGCCCTGGACGATCTCACGGACGCCGGGCCGGAC
GGTCTCGACATCGAAGCCGGCGACCGGCATGACGAAGCGCTCAATCCGGCCGGAGATGCCGAAGATGCGCCAGTGGTGCG
CTTCATCAACAAGATATTGCTGGATGCGATCCGCCGCGGCTCCTCGGACCTGCACTTCGAGCCTTACGAGAAGAGCCACC
GGGTGCGTTTTCGCACCGACGGCATCCTCCATGAGGTGGCCCGTCCGCCCGTCCGGTCGGCGCCGAAGATCGCCGCGCGC
CTGAAAGTGATGGCCGGGCTGGATATCTCCGAGCGGCGTAAACCGCAAGACGGTCGGATCAGGATGAAGCTGCCGAAGGG
CAACGCCATCGACTTTCGGGTCAACACCTTGCCCACGCTGTGGGGCGAAAAGGTGGTGATGCGGATTCTCGACCCGTCCA
GCGCGCAGATGGGTATCGATGCCCTTGGCTACGAAGAGAGCCAGAAGGCGCTCTACCTGGAGGCACTGACCCAGCCGCAG
GGCATGATCCTGGTGACCGGTCCGACCGGCTCGGGCAAAACGGTGTCCTTGTATACCGGCCTGAACATTCTCAATACCAC
GGAGGTGAACATCTCCACCGTCGAGGACCCGGTGGAAATCAACCTGGAAGGCATCAACCAGGTCAACGTCAACCCACGCC
AGGGCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGATATCATCATGGTCGGCGAGATCCGCGAC
CTGGAAACCGCGGAGATCGCCATCAAGGCCGCGCAGACCGGCCACATGGTGATGTCCACCCTGCACACCAATAGCGCGGC
GGAAACTCTGACCCGCCTGCGCAATATGGGCGTGCCCTCCTTCAATATCGCCACCTCGGTGAACCTGATCATCGCCCAGC
GCCTAGCGCGCAAGCTGTGCGCCTGCAAGCAAGCGGTGGACATTCCCCACGAAACGCTGCTCGCCGAGGGATTTCCGGAA
AAGCGCATCGGCGTCTTCAGGCTTTATGCCCCGACCGGTTGCGAGAACTGCAACGGCGGCTACAAGGGCCGGGTCGGCAT
TTATGAAGTGGTTAAAATCACTCCGGCCCTGCAGCGCATTATCATGGGGGACGGCAACTCCATCGATATCGCCCGGCAAA
TGCGCGCCGAGGGTTTCAACGACCTGCGCGCATCGGCCCTGCAAAAAGCAATGCAGGGCGTCACCAGCCTGGAAGAAGTC
AACCGCGTCACCAAGGACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.741

100

0.594
  pilB Acinetobacter baylyi ADP1

56.89

100

0.569
  pilB Legionella pneumophila strain ERS1305867

52.89

100

0.534
  pilF Neisseria gonorrhoeae MS11

49.735

99.823

0.496
  pilB Vibrio parahaemolyticus RIMD 2210633

48.785

100

0.496
  pilB Vibrio cholerae strain A1552

48.759

99.647

0.486
  pilB Vibrio campbellii strain DS40M4

50.763

92.58

0.47
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.414

93.993

0.38
  pilF Thermus thermophilus HB27

37.478

99.47

0.373
  pilB/pilB1 Synechocystis sp. PCC 6803

40.27

91.696

0.369

Multiple sequence alignment