Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACGTJS_RS07350 Genome accession   NZ_CP171363
Coordinates   1499318..1501021 (+) Length   567 a.a.
NCBI ID   WP_410471606.1    Uniprot ID   -
Organism   Faucicola mancuniensis strain GVCNT2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1494318..1506021
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACGTJS_RS07325 (ACGTJS_07325) ppx 1494687..1496198 (+) 1512 WP_410471603.1 exopolyphosphatase -
  ACGTJS_RS07330 (ACGTJS_07330) - 1496260..1497054 (+) 795 WP_315044192.1 acetyl-CoA carboxylase carboxyltransferase subunit alpha -
  ACGTJS_RS07335 (ACGTJS_07335) - 1497079..1497570 (-) 492 WP_410471604.1 GNAT family N-acetyltransferase -
  ACGTJS_RS07340 (ACGTJS_07340) - 1497579..1497872 (-) 294 WP_315044197.1 DUF1778 domain-containing protein -
  ACGTJS_RS07345 (ACGTJS_07345) tilS 1497946..1499175 (+) 1230 WP_410471605.1 tRNA lysidine(34) synthetase TilS -
  ACGTJS_RS07350 (ACGTJS_07350) pilB 1499318..1501021 (+) 1704 WP_410471606.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACGTJS_RS07355 (ACGTJS_07355) pilC 1501077..1502300 (+) 1224 WP_315040990.1 type II secretion system F family protein Machinery gene
  ACGTJS_RS07360 (ACGTJS_07360) pilD 1502300..1503211 (+) 912 WP_315040991.1 A24 family peptidase Machinery gene
  ACGTJS_RS07365 (ACGTJS_07365) coaE 1503250..1503867 (+) 618 WP_315040992.1 dephospho-CoA kinase -
  ACGTJS_RS07385 (ACGTJS_07385) tpiA 1504403..1505209 (+) 807 WP_315040993.1 triose-phosphate isomerase -
  ACGTJS_RS07390 (ACGTJS_07390) secG 1505240..1505545 (+) 306 WP_315040994.1 preprotein translocase subunit SecG -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62454.93 Da        Isoelectric Point: 4.9445

>NTDB_id=1060917 ACGTJS_RS07350 WP_410471606.1 1499318..1501021(+) (pilB) [Faucicola mancuniensis strain GVCNT2]
MSAFPNFGGIALQLVQAGIVSESAMQKAIGEAQQNKKNLIAYLVEEKIASPDKVATLISREFGDALIDLDALSPEYIPKN
AVEEKIIQEFSILPIFQRGNRLFVAMSDPTYVEALDALKFGTRLMVETVVAEHNKIRSMIEKVFSASALSDFGDFNGADF
GEDDKPDDGVGETTLDTTDDAPVVKFVNSMLIKAITMGASDLHFEPYEKVYRVRFRVDGVLQKIANPPLQLASKISARLK
VISQMDISERRIPQDGRIKLKISKDRAIDFRVNSCPTLFGEKLVLRILDPSSAMLGIDALGYEPDQKKLFMEALDKPQGM
LLITGPTGSGKTVSLYTGLNILNTVETNISTAEDPVEINLEGINQVNVNNKVGLTFASALKAFLRQDPDVVMVGEIRDLE
TAEIAIKAAQTGHMVLSTLHTNSAPETLTRLRNMGVASFNIATSVNLVIAQRLARRLCKVCKKPTDVPRQSLLEMGFTEE
DLDNPENVIYEPVGCSECRDGYKGRVGVYEVLKITPEISRIIMEDGNAIQIKDIALTQGFRDLRRSGIMKVLQGLTSVQE
MYRVTSE

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=1060917 ACGTJS_RS07350 WP_410471606.1 1499318..1501021(+) (pilB) [Faucicola mancuniensis strain GVCNT2]
ATGTCTGCATTTCCTAATTTTGGTGGCATTGCGTTGCAATTGGTTCAGGCAGGTATTGTCAGTGAATCTGCCATGCAAAA
GGCAATTGGCGAAGCACAACAAAATAAAAAGAATTTGATTGCTTATTTGGTAGAAGAAAAAATTGCCAGTCCTGATAAAG
TGGCGACTTTGATTTCTCGTGAGTTTGGCGATGCACTGATTGATTTGGACGCATTAAGTCCAGAGTATATTCCAAAAAAT
GCGGTAGAAGAAAAAATTATACAAGAGTTTAGTATTCTGCCAATTTTTCAGCGTGGTAATCGTTTGTTTGTGGCGATGAG
CGACCCAACTTATGTTGAAGCATTAGATGCGTTAAAGTTCGGCACACGTTTGATGGTAGAAACAGTCGTTGCTGAGCATA
ACAAAATCAGGTCGATGATTGAAAAGGTTTTTTCCGCATCAGCCTTGTCAGATTTTGGTGATTTTAATGGGGCAGATTTT
GGTGAAGATGACAAGCCTGATGACGGTGTTGGCGAGACGACACTGGATACGACAGATGATGCCCCTGTGGTAAAATTTGT
GAACAGTATGCTTATCAAAGCGATTACGATGGGAGCATCGGATTTGCACTTTGAGCCTTATGAAAAAGTGTATCGTGTGC
GTTTTCGTGTTGACGGTGTGTTACAAAAAATTGCCAATCCGCCTTTGCAGTTGGCAAGTAAAATTTCGGCACGTTTGAAA
GTCATTTCACAAATGGATATTTCAGAAAGACGTATCCCACAAGATGGTCGTATTAAGCTTAAAATTTCCAAAGACCGTGC
GATTGACTTTCGTGTGAACTCATGTCCGACGCTTTTTGGCGAAAAATTGGTACTGCGTATTTTGGATCCATCGTCTGCGA
TGTTGGGTATTGATGCGTTGGGTTATGAGCCTGACCAGAAAAAACTGTTTATGGAAGCCCTTGATAAACCGCAAGGTATG
CTATTGATTACAGGGCCGACAGGTTCTGGTAAAACGGTTTCGCTTTATACTGGTTTAAATATTTTAAATACCGTTGAAAC
CAATATTTCAACCGCTGAAGACCCTGTTGAGATTAACTTAGAAGGGATTAATCAGGTCAATGTGAACAACAAAGTGGGGC
TAACCTTTGCCAGTGCCTTAAAAGCCTTCTTACGTCAGGACCCTGACGTAGTTATGGTTGGTGAGATTCGTGACCTTGAA
ACTGCCGAAATTGCGATTAAAGCTGCGCAAACAGGTCACATGGTATTGTCTACCCTGCATACCAATTCAGCACCAGAAAC
ATTGACTCGTTTGCGGAATATGGGGGTGGCTTCATTTAATATTGCCACTTCGGTAAACTTGGTGATTGCACAGCGTCTGG
CTCGCCGTCTTTGTAAAGTGTGTAAAAAGCCAACTGATGTACCAAGACAAAGTTTGCTTGAAATGGGATTTACTGAAGAA
GATTTGGATAATCCAGAAAATGTGATTTATGAGCCTGTGGGCTGTTCTGAGTGCCGTGATGGCTATAAGGGTCGTGTGGG
GGTTTATGAAGTGCTAAAAATCACCCCAGAAATCTCAAGAATTATTATGGAAGACGGTAATGCCATTCAAATTAAAGACA
TCGCCTTAACACAAGGTTTTAGAGATTTACGCCGTTCTGGCATTATGAAAGTGTTACAGGGTTTGACCAGTGTACAAGAA
ATGTATCGTGTAACCAGCGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(189-456)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

67.016

100

0.677
  pilB Acinetobacter baylyi ADP1

64.799

100

0.653
  pilB Legionella pneumophila strain ERS1305867

53.321

95.591

0.51
  pilF Neisseria gonorrhoeae MS11

49.107

98.765

0.485
  pilB Vibrio cholerae strain A1552

48.214

98.765

0.476
  pilB Vibrio parahaemolyticus RIMD 2210633

47.397

98.236

0.466
  pilB Vibrio campbellii strain DS40M4

46.763

98.06

0.459
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.526

100

0.406
  pilB/pilB1 Synechocystis sp. PCC 6803

36.187

100

0.395

Multiple sequence alignment