Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ACGK9W_RS21065 Genome accession   NZ_CP171359
Coordinates   4511868..4513949 (+) Length   693 a.a.
NCBI ID   WP_405418548.1    Uniprot ID   -
Organism   Marinobacter flavimaris strain ZYH30     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4506868..4518949
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACGK9W_RS21045 (ACGK9W_21045) pilM 4509016..4510077 (+) 1062 WP_405418545.1 pilus assembly protein PilM Machinery gene
  ACGK9W_RS21050 (ACGK9W_21050) - 4510077..4510640 (+) 564 WP_053114373.1 PilN domain-containing protein -
  ACGK9W_RS21055 (ACGK9W_21055) - 4510640..4511251 (+) 612 WP_008170795.1 type 4a pilus biogenesis protein PilO -
  ACGK9W_RS21060 (ACGK9W_21060) - 4511254..4511802 (+) 549 WP_008170793.1 pilus assembly protein PilP -
  ACGK9W_RS21065 (ACGK9W_21065) pilQ 4511868..4513949 (+) 2082 WP_405418548.1 type IV pilus secretin PilQ Machinery gene
  ACGK9W_RS21070 (ACGK9W_21070) aroK 4514086..4514652 (+) 567 WP_405418549.1 shikimate kinase AroK -
  ACGK9W_RS21075 (ACGK9W_21075) aroB 4514645..4515739 (+) 1095 WP_405418551.1 3-dehydroquinate synthase -
  ACGK9W_RS21080 (ACGK9W_21080) - 4515795..4517318 (+) 1524 WP_405418553.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 693 a.a.        Molecular weight: 74473.43 Da        Isoelectric Point: 4.2570

>NTDB_id=1060880 ACGK9W_RS21065 WP_405418548.1 4511868..4513949(+) (pilQ) [Marinobacter flavimaris strain ZYH30]
MFRKLNVYVSVIAFGLLSGLANAVTLEDVSFSSLPGERLEVTLQFDGAPPEPSGYTIERPARIAVDLRDTTSGLESRSVP
LGSGNAQSMTVVETKDRTRLIFNLVELVPYDTVRSGNSLVMTIGGQADGVVASSSAPASQSSSTSGSMSANALAGVDFRR
GKDGEGRVLVDLGSSSTPVDLTERAGKIRLTMDGIEVPSDLRRRLDVTDFATPVTRIDTFVEDGNAVVEITPEGNYDYIA
YQSGSQFTVSVEELSQEEAESRREEKFPYTGDKLSLNFQDIEVRSVLQLIADFTGLNLVASDTVGGSITLRLQNVPWDQA
LDLILKTKGLDKRQIGNVLLVAPADEIAAREKLELETNKQIAELAPVRLDIIQVNYAKAADIVALIKEDEELISDRGFVS
SDVRTNTISVRETAEKLDEIRRLVSTWDVPVRQVSIEARIVRAQTNVAENLGVRWGGAAYDNEGNSIVSIGGSRDSVGEA
VNGSTAGTETGFRNSISFPGALAVDLGVTGEGASSFAIGWGSDDFLVDLELSALESDGQAEVVSQPRVVTADRQTASIKS
GEEIPYQEAASSGATSVSFKEAVLSLEVTPQITPDDKIIMDLVVNQDSRGEVTAGIPSINTNEVTTQVLVGNGETVVLGG
IFQSEVATQTTKTPFLGDIPYLGRIFKRTEHIDERSELLIFITPKIIKNDLLR

Nucleotide


Download         Length: 2082 bp        

>NTDB_id=1060880 ACGK9W_RS21065 WP_405418548.1 4511868..4513949(+) (pilQ) [Marinobacter flavimaris strain ZYH30]
ATGTTCAGAAAACTCAATGTATACGTCAGCGTGATTGCTTTTGGGTTGTTATCCGGCCTGGCCAACGCGGTCACGCTGGA
AGACGTGTCGTTTTCGTCGCTACCGGGCGAGCGGCTTGAGGTGACACTGCAATTTGATGGGGCACCGCCGGAACCTTCCG
GTTATACCATTGAGCGTCCCGCTCGCATTGCGGTTGATCTTCGGGACACGACCAGTGGTCTTGAAAGTCGCAGTGTGCCT
CTGGGATCAGGCAACGCCCAGAGCATGACCGTGGTGGAAACCAAGGACCGTACCCGGCTGATATTCAACCTGGTCGAGCT
GGTGCCCTATGACACAGTTCGTTCGGGCAACTCCCTGGTCATGACAATTGGCGGCCAGGCCGATGGCGTTGTCGCGAGTT
CCTCTGCTCCCGCGTCTCAAAGCAGCTCAACATCGGGCTCCATGTCTGCCAACGCTCTGGCCGGTGTGGATTTCCGTCGG
GGCAAGGACGGTGAAGGTCGCGTTCTTGTTGATCTGGGTAGCTCCAGCACGCCGGTGGACCTGACAGAGCGCGCCGGTAA
GATTCGCCTGACGATGGACGGTATTGAAGTACCCTCGGACCTGCGGCGCCGACTCGATGTGACAGATTTCGCCACCCCGG
TTACCCGTATTGACACCTTTGTGGAAGATGGCAATGCCGTGGTCGAGATCACCCCCGAGGGGAATTACGACTACATTGCC
TACCAGTCCGGCAGCCAGTTTACTGTCAGCGTTGAAGAACTGAGCCAGGAAGAGGCGGAGTCCCGCAGGGAAGAGAAGTT
CCCGTATACCGGTGACAAGCTGTCCCTGAACTTCCAGGATATTGAAGTGCGCTCGGTCCTGCAGCTGATTGCTGACTTCA
CCGGTCTGAACCTGGTTGCCAGTGATACGGTAGGCGGCAGCATTACTCTGCGTCTGCAGAATGTTCCCTGGGATCAGGCG
CTGGATCTGATTCTCAAGACCAAAGGCCTCGATAAGCGTCAGATCGGCAACGTATTGCTGGTAGCGCCTGCAGACGAAAT
CGCAGCGCGTGAGAAGCTTGAGCTCGAAACCAACAAGCAGATTGCCGAACTGGCCCCGGTTCGTCTGGACATCATTCAGG
TTAACTACGCCAAAGCGGCTGATATTGTTGCGTTGATCAAGGAAGACGAAGAGCTGATCTCTGATCGCGGATTCGTCTCC
TCTGATGTTCGTACCAACACGATCAGTGTTCGTGAAACTGCCGAGAAGCTTGATGAAATTCGCCGTCTGGTATCGACATG
GGATGTGCCGGTGCGTCAGGTGTCTATCGAGGCTCGGATTGTTCGCGCCCAGACCAACGTGGCAGAGAACCTCGGTGTTC
GCTGGGGTGGTGCCGCGTACGATAATGAGGGAAATAGTATTGTGTCAATTGGAGGTTCGCGCGACTCAGTTGGTGAGGCG
GTGAATGGTTCAACCGCAGGAACTGAAACTGGATTCAGAAATAGTATTTCTTTCCCAGGCGCCCTTGCGGTCGACCTCGG
AGTGACTGGTGAAGGTGCCTCTTCCTTCGCCATCGGGTGGGGCAGCGACGACTTCCTGGTAGATCTGGAGCTGTCAGCCC
TTGAGAGTGATGGTCAAGCGGAAGTGGTTTCCCAGCCCCGGGTTGTGACTGCCGATCGTCAGACGGCGTCGATCAAGTCT
GGTGAGGAAATTCCCTATCAGGAAGCGGCCTCCAGCGGTGCGACGTCTGTTTCTTTCAAGGAAGCCGTGCTCTCCCTGGA
AGTAACGCCGCAGATTACACCGGATGACAAGATCATCATGGATCTGGTGGTCAATCAGGACTCCCGAGGCGAGGTGACTG
CTGGTATTCCGTCGATCAATACCAACGAAGTCACCACCCAGGTTCTGGTTGGTAACGGTGAGACAGTTGTTCTGGGCGGT
ATCTTCCAGTCGGAAGTTGCGACCCAGACCACCAAAACGCCGTTCCTCGGAGATATTCCCTACCTGGGCCGTATCTTCAA
GCGCACCGAACACATTGATGAGCGCAGTGAACTGCTGATCTTCATCACACCGAAGATCATCAAGAACGATCTGCTGCGCT
AA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(297-343)

AMIN domain (AMIN)

(38-121)

Bacterial type II/III secretion system short domain (Secretin_N)

(371-433)

Bacterial type II and III secretion system protein (Secretin)

(533-688)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

49.3

100

0.508
  comQ Acinetobacter baylyi ADP1

41.234

100

0.424
  pilQ Acinetobacter baumannii strain A118

39.672

100

0.418
  pilQ Acinetobacter baumannii D1279779

39.672

100

0.418
  pilQ Legionella pneumophila strain Lp02

40.147

98.124

0.394
  pilQ Legionella pneumophila strain ERS1305867

40.147

98.124

0.394

Multiple sequence alignment