Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACF3M1_RS03280 Genome accession   NZ_CP171131
Coordinates   736635..738356 (-) Length   573 a.a.
NCBI ID   WP_394003902.1    Uniprot ID   -
Organism   Luteimonas sp. WGS1318     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 731635..743356
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACF3M1_RS03260 (ACF3M1_03260) - 732237..733184 (+) 948 WP_394003897.1 Nudix family hydrolase -
  ACF3M1_RS03265 (ACF3M1_03265) coaE 733589..734215 (-) 627 WP_394003899.1 dephospho-CoA kinase -
  ACF3M1_RS03270 (ACF3M1_03270) pilD 734232..735095 (-) 864 WP_394003900.1 prepilin peptidase Machinery gene
  ACF3M1_RS03275 (ACF3M1_03275) pilC 735124..736278 (-) 1155 WP_394005158.1 type II secretion system F family protein Machinery gene
  ACF3M1_RS03280 (ACF3M1_03280) pilB 736635..738356 (-) 1722 WP_394003902.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACF3M1_RS03285 (ACF3M1_03285) - 738494..739327 (+) 834 WP_394003903.1 glycosyltransferase family 2 protein -
  ACF3M1_RS03290 (ACF3M1_03290) - 739332..740498 (+) 1167 WP_394003905.1 DegT/DnrJ/EryC1/StrS family aminotransferase -
  ACF3M1_RS03295 (ACF3M1_03295) - 740527..741174 (+) 648 WP_394003906.1 NeuD/PglB/VioB family sugar acetyltransferase -
  ACF3M1_RS03300 (ACF3M1_03300) - 741253..742731 (+) 1479 WP_394003907.1 lipopolysaccharide biosynthesis protein -

Sequence

Protein


Download         Length: 573 a.a.        Molecular weight: 62448.54 Da        Isoelectric Point: 5.5986

>NTDB_id=1060268 ACF3M1_RS03280 WP_394003902.1 736635..738356(-) (pilB) [Luteimonas sp. WGS1318]
MSAVATPNLIGITGIARRLVLDGALDEAAARDALEKATAARKQIAAYLREQKLVTAAQMAAANSIEFGMPVFDPAVMDPQ
ASAVKLVSEELLGKHGVLPLFKRGSRLFVGTADPTNTHALDEIKFHTNLTVEPILVDEDSIRRTLEVWLQASDSLDDSLE
DADGLESLDVGGDEDSKTDNVADGKDDTPVVKFVNKVLVDAIKKGASDIHFEPYETDYRVRLRIDGILKQSAKMPVKLNQ
RITARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDASAAKLGIDKLGYEPDQQALFLEA
IHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDIIMV
GEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTISRLMNMGIAPYNITSSVTLVIAQRLARRLCSKCKRLANIPEHALL
SEGYTEDQVRAGIELYEAVGCDDCTNGYKGRVGVYQVMPMSEAIQEIILQGGNAMQIAEAAQRSGVSDLRQSALEKARQG
LTSLAEINRVTKD

Nucleotide


Download         Length: 1722 bp        

>NTDB_id=1060268 ACF3M1_RS03280 WP_394003902.1 736635..738356(-) (pilB) [Luteimonas sp. WGS1318]
ATGAGCGCCGTTGCGACCCCGAACCTGATCGGCATCACCGGTATCGCCCGCCGCCTCGTACTGGACGGCGCGCTCGACGA
AGCGGCGGCGCGCGATGCGCTGGAGAAGGCCACGGCGGCGCGCAAGCAGATTGCGGCCTACCTGCGCGAGCAGAAGCTGG
TGACCGCGGCGCAGATGGCGGCCGCGAACTCGATCGAGTTCGGCATGCCGGTCTTCGACCCTGCCGTGATGGACCCACAG
GCCAGCGCGGTCAAGCTGGTCAGCGAGGAACTGCTCGGCAAGCACGGGGTGCTGCCGCTGTTCAAGCGTGGCAGCCGCTT
GTTCGTGGGCACGGCGGACCCGACGAACACGCATGCGCTGGACGAGATCAAGTTCCACACCAACCTGACGGTCGAGCCGA
TCCTGGTCGACGAGGACAGCATCCGCCGCACATTGGAGGTGTGGCTGCAGGCCTCAGATTCGCTGGACGATTCGCTGGAG
GACGCCGACGGCCTCGAATCACTGGACGTCGGCGGCGACGAGGACAGCAAGACCGACAATGTCGCCGACGGCAAGGACGA
CACGCCGGTCGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCCATCAAGAAGGGCGCCTCGGACATCCATTTCGAGCCCT
ACGAGACCGATTACCGCGTGCGCCTGCGCATCGACGGCATCCTCAAACAGTCGGCGAAGATGCCGGTCAAGCTCAACCAG
CGCATCACCGCGCGCCTGAAGGTCATGGCGCAGCTCGACATCGCCGAGAAGCGCGTGCCGCAGGACGGGCGCATCAAGCT
GAACCTGTCGAAGACCAAGCAGATCGACTTCCGTGTCAGCACCCTGCCCACGCTGTTCGGTGAGAAGGTGGTGCTGCGTA
TCCTCGACGCCAGCGCGGCGAAGCTCGGCATCGACAAGCTGGGTTACGAGCCCGACCAGCAGGCGTTGTTTCTCGAGGCC
ATCCACAAGCCCTACGGCATGGTGCTCGTCACCGGCCCCACCGGCTCGGGCAAGACCGTGTCGTTGTACACGGCGCTCGG
CATCCTCAACGACGAGACCCGCAACATCTCGACCGCCGAGGACCCGGTGGAAATCCGCCTGCCCGGCGTCAACCAGGTGC
AGCAGAACAACAAGCGCGGCATGACCTTCGCCGCGGCGCTGAGGTCGTTCCTGCGCCAGGACCCGGACATCATTATGGTC
GGCGAAATCCGCGACCTCGAAACCGCGGAAATCGCGATCAAGGCCGCTCAGACCGGCCACATGGTGCTGTCCACGCTGCA
TACCAACGATGCGCCGCAGACCATCTCGCGCCTGATGAACATGGGCATCGCGCCGTACAACATCACGTCGTCGGTGACAC
TGGTCATCGCGCAGCGCCTGGCGCGCCGGCTGTGCTCCAAGTGCAAGCGTCTCGCCAATATTCCCGAACACGCGCTGCTG
AGCGAGGGCTATACCGAGGACCAGGTGCGCGCAGGCATCGAGCTCTACGAGGCCGTGGGTTGCGACGACTGCACCAACGG
CTACAAGGGCCGTGTGGGCGTCTATCAGGTGATGCCGATGTCCGAGGCGATCCAGGAGATCATCCTGCAGGGCGGCAACG
CCATGCAGATCGCCGAGGCGGCGCAGAGATCCGGGGTGAGCGATCTGCGCCAGTCGGCGCTGGAGAAGGCGCGTCAGGGG
CTGACCAGCCTGGCCGAGATCAATCGCGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(196-463)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.556

100

0.558
  pilB Acinetobacter baumannii D1279779

55.401

100

0.555
  pilB Legionella pneumophila strain ERS1305867

52.473

98.778

0.518
  pilB Vibrio cholerae strain A1552

48.682

99.302

0.483
  pilF Neisseria gonorrhoeae MS11

47.78

98.255

0.469
  pilB Vibrio parahaemolyticus RIMD 2210633

48.101

96.51

0.464
  pilB Vibrio campbellii strain DS40M4

51.633

85.515

0.442
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.817

95.113

0.379
  pilB/pilB1 Synechocystis sp. PCC 6803

42.063

87.958

0.37
  pilF Thermus thermophilus HB27

40.194

89.878

0.361

Multiple sequence alignment