Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   ACF3M1_RS03270 Genome accession   NZ_CP171131
Coordinates   734232..735095 (-) Length   287 a.a.
NCBI ID   WP_394003900.1    Uniprot ID   -
Organism   Luteimonas sp. WGS1318     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 729232..740095
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACF3M1_RS03260 (ACF3M1_03260) - 732237..733184 (+) 948 WP_394003897.1 Nudix family hydrolase -
  ACF3M1_RS03265 (ACF3M1_03265) coaE 733589..734215 (-) 627 WP_394003899.1 dephospho-CoA kinase -
  ACF3M1_RS03270 (ACF3M1_03270) pilD 734232..735095 (-) 864 WP_394003900.1 prepilin peptidase Machinery gene
  ACF3M1_RS03275 (ACF3M1_03275) pilC 735124..736278 (-) 1155 WP_394005158.1 type II secretion system F family protein Machinery gene
  ACF3M1_RS03280 (ACF3M1_03280) pilB 736635..738356 (-) 1722 WP_394003902.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACF3M1_RS03285 (ACF3M1_03285) - 738494..739327 (+) 834 WP_394003903.1 glycosyltransferase family 2 protein -

Sequence

Protein


Download         Length: 287 a.a.        Molecular weight: 31213.07 Da        Isoelectric Point: 7.9568

>NTDB_id=1060266 ACF3M1_RS03270 WP_394003900.1 734232..735095(-) (pilD) [Luteimonas sp. WGS1318]
MAFLDQNPALGFPLAAGLGLLVGSFLNVVILRLPRRMEWAWKRDSREILGEPELYDPPPPGIVVERSHCPHCGHQLSWYE
NIPVLSWLALRGKCRSCKAPISIQYPAVELLTMLLFVACVWRFGFGWQGFGALVLTGFLVALSGIDIRTQLLPDSLTLPL
MWLGLIASLDTLYMPAKPALLGAIVGYASLWSVWWLFKQATGKEGMGHGDFKLLAALGAWCGLTGLLPIILISAVAGAII
GSILLAVQGRDRATPIPFGPYLAIAGWVTFMWGDDLVGSYMRVAGLA

Nucleotide


Download         Length: 864 bp        

>NTDB_id=1060266 ACF3M1_RS03270 WP_394003900.1 734232..735095(-) (pilD) [Luteimonas sp. WGS1318]
ATGGCGTTTCTCGACCAGAACCCCGCACTCGGCTTTCCGCTCGCCGCCGGCCTCGGCCTGCTGGTGGGCAGCTTCCTCAA
CGTGGTGATCCTGCGCCTGCCGCGGCGCATGGAATGGGCGTGGAAGCGCGACAGCCGCGAGATCCTCGGTGAGCCGGAGC
TCTACGACCCGCCCCCGCCGGGCATTGTCGTCGAGCGCTCGCATTGCCCGCACTGCGGGCACCAGCTGTCGTGGTACGAG
AACATTCCGGTCCTGAGCTGGCTGGCGCTGCGCGGCAAGTGCCGCAGCTGCAAGGCGCCGATCTCGATCCAGTACCCGGC
GGTCGAACTGCTGACGATGCTGCTGTTCGTCGCCTGCGTGTGGCGCTTCGGCTTCGGCTGGCAGGGCTTCGGTGCGCTGG
TGCTGACTGGTTTTCTGGTCGCGCTGTCGGGTATCGACATCCGCACCCAGCTGCTGCCTGATTCGCTGACGCTGCCCCTA
ATGTGGCTCGGGCTGATCGCCAGCCTGGATACGCTCTACATGCCCGCCAAGCCCGCGCTGCTGGGCGCGATCGTCGGGTA
CGCGAGCCTGTGGTCGGTGTGGTGGCTGTTCAAGCAGGCCACCGGCAAGGAAGGCATGGGTCATGGCGATTTCAAGCTGC
TCGCCGCGCTGGGCGCCTGGTGCGGCCTGACCGGTCTGCTGCCGATCATCCTGATCTCGGCCGTGGCTGGCGCGATCATC
GGCTCGATCCTGCTCGCCGTGCAGGGCCGCGACCGCGCCACGCCGATCCCGTTCGGCCCGTATCTGGCCATCGCCGGCTG
GGTGACCTTCATGTGGGGCGACGACCTGGTCGGCAGTTACATGCGCGTCGCCGGACTGGCGTAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(133-241)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(18-123)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Acinetobacter baumannii D1279779

48.958

100

0.491
  pilD Vibrio cholerae strain A1552

50.182

95.819

0.481
  pilD Acinetobacter nosocomialis M2

48.936

98.258

0.481
  pilD Vibrio campbellii strain DS40M4

47.887

98.955

0.474
  pilD Neisseria gonorrhoeae MS11

47.547

92.334

0.439

Multiple sequence alignment