Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACF3OJ_RS03645 Genome accession   NZ_CP171111
Coordinates   701996..703690 (+) Length   564 a.a.
NCBI ID   WP_311335432.1    Uniprot ID   -
Organism   Cardiobacterium hominis strain WGS1530     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 696996..708690
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACF3OJ_RS03610 (ACF3OJ_03610) fni 697201..698223 (-) 1023 WP_392406483.1 type 2 isopentenyl-diphosphate Delta-isomerase -
  ACF3OJ_RS03615 (ACF3OJ_03615) - 698220..699266 (-) 1047 WP_392406484.1 hydroxymethylglutaryl-CoA reductase -
  ACF3OJ_RS03625 (ACF3OJ_03625) rpsI 699620..700009 (-) 390 WP_004139624.1 30S ribosomal protein S9 -
  ACF3OJ_RS03630 (ACF3OJ_03630) rplM 700019..700450 (-) 432 WP_048716663.1 50S ribosomal protein L13 -
  ACF3OJ_RS03635 (ACF3OJ_03635) recO 700676..701344 (-) 669 WP_392406485.1 DNA repair protein RecO -
  ACF3OJ_RS03640 (ACF3OJ_03640) - 701409..701999 (+) 591 WP_179123632.1 aminodeoxychorismate/anthranilate synthase component II -
  ACF3OJ_RS03645 (ACF3OJ_03645) pilB 701996..703690 (+) 1695 WP_311335432.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACF3OJ_RS03650 (ACF3OJ_03650) - 703708..704925 (+) 1218 WP_079541850.1 type II secretion system F family protein -
  ACF3OJ_RS03655 (ACF3OJ_03655) - 704925..705806 (+) 882 WP_079541852.1 A24 family peptidase -
  ACF3OJ_RS03660 (ACF3OJ_03660) coaE 705808..706431 (+) 624 WP_079541854.1 dephospho-CoA kinase -
  ACF3OJ_RS03665 (ACF3OJ_03665) - 706480..706944 (-) 465 WP_079541856.1 S-ribosylhomocysteine lyase -
  ACF3OJ_RS03670 (ACF3OJ_03670) yegQ 707101..708441 (+) 1341 WP_079541858.1 tRNA 5-hydroxyuridine modification protein YegQ -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 62635.52 Da        Isoelectric Point: 5.4176

>NTDB_id=1060136 ACF3OJ_RS03645 WP_311335432.1 701996..703690(+) (pilB) [Cardiobacterium hominis strain WGS1530]
MSDYDPLISYMVQHKICTPEQIREVQISAQKKRGSFLQQLLSDGLIDGSRLSQINREVYNLPQVMLRELDVRRELTAQFP
ETVVRKHMALPIYANGGRLFIATFDPSNRIMLEEFKYQGKFTSVEPIVADIGVIEALIEDHYAGVGGMESVFEDENQEQD
LDAISEALGNIGANEEEAPVVRFVSGMLMDAIRTGASDLHFEPYENTYRVRFRRDGVLQEVAAPPPSIANRITARLKVMA
NLDIAEKRVPQDGRIKLQVSETKAIDFRVSSLPTLWGEKIVLRILDSSAAKLNIDVLGFEPDQKQHYLNAISKPQGLVLV
TGPTGSGKTVSLYTGLSILNKPTVNISTAEDPVEINLPGINQVNVNTKTGLDFAAALKAFLRQDPDIIMVGEIRDITTGE
IAVKAAQTGHLVLSTLHTNDVPQTIARLVNIGIPSYNIAASVNLIMAQRLTRRLCSHCKTRDRKHSHDELIALGFAEDEI
DDLRIYAPKGCERCSYQGYRGRAGIYQVVPISESLSEMILHNAAAADIAEQCQKEGYWDLRQSALNKVRQGLTSIEEILR
VTTD

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=1060136 ACF3OJ_RS03645 WP_311335432.1 701996..703690(+) (pilB) [Cardiobacterium hominis strain WGS1530]
ATGAGTGATTATGATCCTTTAATCAGCTATATGGTACAGCACAAAATATGCACACCCGAGCAAATTAGGGAAGTGCAAAT
TAGTGCGCAGAAAAAACGGGGCAGTTTTTTACAACAACTACTGAGTGATGGTTTGATAGACGGTTCACGGCTATCGCAAA
TTAATCGAGAAGTTTACAACTTGCCACAAGTTATGCTGCGCGAATTGGATGTTAGACGTGAATTAACTGCGCAATTCCCG
GAAACCGTTGTGCGAAAACACATGGCCCTTCCTATCTATGCTAATGGCGGACGGCTATTTATTGCGACGTTTGATCCTTC
AAACCGTATTATGCTGGAGGAGTTTAAATATCAAGGTAAATTTACTTCTGTTGAACCAATTGTGGCGGATATAGGTGTTA
TAGAAGCACTTATAGAAGATCACTACGCTGGTGTGGGTGGGATGGAGAGTGTTTTTGAGGATGAAAATCAAGAACAAGAT
CTGGATGCCATTAGTGAAGCGCTAGGTAATATTGGTGCAAATGAAGAAGAAGCGCCTGTTGTTCGTTTCGTTTCGGGCAT
GCTAATGGATGCCATTCGTACTGGGGCTTCGGATTTGCACTTTGAGCCTTATGAAAATACTTATCGGGTGCGATTCCGCC
GAGACGGTGTCTTGCAAGAAGTTGCAGCACCTCCTCCCAGTATTGCAAACCGTATTACTGCACGCTTAAAAGTTATGGCA
AATTTGGATATTGCAGAGAAGCGTGTTCCTCAGGATGGGCGTATTAAACTTCAGGTTTCTGAAACAAAAGCTATCGATTT
CCGGGTTAGCTCATTGCCAACGCTATGGGGAGAAAAAATCGTACTACGTATTCTCGATAGTTCAGCAGCTAAACTAAATA
TCGATGTTCTTGGCTTTGAACCAGATCAGAAGCAGCATTATCTCAATGCTATTAGTAAACCCCAAGGTCTAGTGTTGGTT
ACCGGCCCTACTGGCTCAGGTAAAACTGTTTCTCTGTATACCGGGCTATCTATTCTGAATAAACCAACTGTTAATATTTC
TACTGCTGAAGATCCTGTGGAAATAAACTTACCCGGTATTAACCAGGTTAACGTAAATACCAAGACAGGATTGGATTTTG
CAGCCGCGCTAAAAGCCTTTTTGCGTCAGGATCCTGATATCATCATGGTTGGGGAGATTCGTGATATCACGACAGGTGAA
ATTGCCGTAAAAGCGGCGCAAACGGGGCACTTGGTTTTATCTACATTGCACACTAACGATGTACCACAAACGATTGCACG
TTTGGTGAATATCGGGATTCCCTCCTATAACATTGCTGCTAGTGTAAATTTAATTATGGCACAGCGATTGACGCGCCGCT
TGTGTAGCCACTGCAAAACGCGTGACCGAAAACATAGTCATGATGAGTTGATCGCCTTAGGCTTTGCCGAAGATGAGATT
GATGATCTACGGATTTATGCTCCTAAAGGGTGTGAACGTTGCAGTTATCAAGGATATCGTGGTCGCGCGGGTATTTACCA
AGTTGTGCCGATCTCTGAATCGTTGTCAGAAATGATTTTGCATAATGCAGCGGCAGCAGATATTGCTGAACAATGCCAAA
AAGAAGGCTATTGGGATTTACGTCAATCTGCATTGAATAAAGTACGTCAAGGTCTGACCAGTATTGAAGAGATCTTGCGT
GTAACAACTGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(187-453)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.291

88.83

0.482
  pilF Neisseria gonorrhoeae MS11

48.05

100

0.481
  pilB Acinetobacter baylyi ADP1

47.359

100

0.477
  pilB Legionella pneumophila strain ERS1305867

46.561

100

0.468
  pilB Vibrio cholerae strain A1552

47.273

97.518

0.461
  pilB Vibrio campbellii strain DS40M4

47.004

94.681

0.445
  pilB Vibrio parahaemolyticus RIMD 2210633

47.18

94.326

0.445
  pilF Thermus thermophilus HB27

37.123

99.823

0.371
  pilB/pilB1 Synechocystis sp. PCC 6803

39.122

92.908

0.363
  pilB Haemophilus influenzae 86-028NP

38.71

93.44

0.362

Multiple sequence alignment