Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACFX5Z_RS07960 Genome accession   NZ_CP170761
Coordinates   1710836..1711975 (-) Length   379 a.a.
NCBI ID   WP_017766703.1    Uniprot ID   -
Organism   Aeromonas dhakensis strain CWH5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1705836..1716975
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFX5Z_RS07940 (ACFX5Z_07940) feoB 1706070..1708343 (+) 2274 WP_279451157.1 Fe(2+) transporter permease subunit FeoB -
  ACFX5Z_RS07945 (ACFX5Z_07945) - 1708340..1708582 (+) 243 WP_139751944.1 FeoC-like transcriptional regulator -
  ACFX5Z_RS07950 (ACFX5Z_07950) - 1708693..1709172 (-) 480 WP_279451155.1 WbuC family cupin fold metalloprotein -
  ACFX5Z_RS07955 (ACFX5Z_07955) glgC 1709308..1710579 (-) 1272 WP_005302634.1 glucose-1-phosphate adenylyltransferase -
  ACFX5Z_RS07960 (ACFX5Z_07960) pilU 1710836..1711975 (-) 1140 WP_017766703.1 type IVa pilus ATPase TapW Machinery gene
  ACFX5Z_RS07965 (ACFX5Z_07965) - 1712035..1712391 (-) 357 WP_005302630.1 DMT family protein -
  ACFX5Z_RS07970 (ACFX5Z_07970) - 1712505..1713236 (+) 732 WP_024942579.1 ABC transporter substrate-binding protein -
  ACFX5Z_RS07975 (ACFX5Z_07975) btuC 1713335..1714342 (+) 1008 WP_279451153.1 vitamin B12 ABC transporter permease BtuC -
  ACFX5Z_RS07980 (ACFX5Z_07980) - 1714329..1715114 (+) 786 WP_258116684.1 ATP-binding cassette domain-containing protein -
  ACFX5Z_RS07985 (ACFX5Z_07985) - 1715194..1716255 (-) 1062 WP_005302620.1 3-deoxy-7-phosphoheptulonate synthase -
  ACFX5Z_RS07990 (ACFX5Z_07990) - 1716412..1716792 (+) 381 WP_010633544.1 diacylglycerol kinase -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42171.19 Da        Isoelectric Point: 6.2561

>NTDB_id=1059303 ACFX5Z_RS07960 WP_017766703.1 1710836..1711975(-) (pilU) [Aeromonas dhakensis strain CWH5]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGGLRPLSETPLEPGEVASIADAIMDGEQKQQFEKELEMNLAISLPQIGR
FRINIFKQRNEVSLVARNIKTEIPRFEDLKLPTVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAAVEIMLGTHTIRDMVKRGEFGGLKEVMEKSKALGMVTFDSAL
FDLVVEGVIDEEEAVKNADSANNLRLKIKLWKEKGQIASSSDATGWSLEPTKDEKDDLF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=1059303 ACFX5Z_RS07960 WP_017766703.1 1710836..1711975(-) (pilU) [Aeromonas dhakensis strain CWH5]
ATGGAATTGCGAGACATGCTACAGATCCTGGCCAAGCAGGACGGCTCAGACCTCTATCTGTCGACCGGCGCGCCCCCCTG
TGCCAAGTTCAACGGCGGCCTGCGCCCCTTGAGCGAGACGCCACTCGAGCCCGGCGAGGTGGCCAGCATAGCCGACGCCA
TCATGGACGGCGAGCAGAAGCAGCAGTTTGAAAAGGAGCTGGAGATGAACCTCGCCATCTCCCTGCCCCAGATAGGGCGC
TTTCGGATCAACATCTTCAAACAGCGCAACGAAGTCTCTCTGGTGGCGCGCAACATCAAGACCGAGATCCCCCGCTTCGA
GGATTTGAAACTGCCCACAGTGCTGCTCGACACCATCATGGAGAAACGGGGGCTGGTGCTGTTCGTCGGCGGTACCGGTT
CGGGCAAATCCACCTCGCTGGCGGCCCTTATCGACCATCGCAACCGCAACAGCGGCGGCCACATCATCACCATCGAGGAT
CCGGTGGAGTTCGTGCACCGCCACCGCAAGAGCATCATCAACCAACGGGAGGTGGGGGTGGATACCCGCAGCTTCCACGC
CGCCCTGAAAAACACCCTGCGCCAGGCGCCGGACGTGATCCTTATAGGCGAAATTCGCGATCGCGAGACCATGGAGCACG
CGCTGGCCTTCTCGGAAACCGGCCACCTCGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGGAAGAGCGCCGGCCCCAGCTGCTCAACGATCTTGGCAACAACCTCAAGGCGTTTGTCTCCCAGCG
GCTGGTGAAAACCACTGATGGCGGCCGCCGGGCCGCGGTGGAGATCATGCTGGGTACCCACACCATCCGCGACATGGTCA
AGCGCGGCGAGTTTGGCGGCCTCAAGGAGGTGATGGAGAAGTCCAAGGCCCTCGGCATGGTCACCTTCGACAGCGCCCTG
TTCGATCTGGTGGTGGAAGGGGTGATCGACGAGGAGGAGGCGGTGAAGAACGCCGACTCGGCCAACAACCTGCGCCTCAA
GATCAAGCTGTGGAAGGAGAAGGGCCAGATCGCCAGCAGCAGCGATGCCACCGGCTGGAGCCTGGAGCCCACCAAGGACG
AGAAGGACGATCTGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

53.867

98.945

0.533
  pilU Acinetobacter baylyi ADP1

52.958

93.668

0.496
  pilU Vibrio cholerae strain A1552

50

92.348

0.462
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.793

93.14

0.38
  pilT Pseudomonas aeruginosa PAK

40.407

90.765

0.367
  pilT Legionella pneumophila strain ERS1305867

41.317

88.127

0.364
  pilT Legionella pneumophila strain Lp02

41.317

88.127

0.364
  pilT Pseudomonas stutzeri DSM 10701

39.826

90.765

0.361

Multiple sequence alignment