Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   ACFW98_RS06235 Genome accession   NZ_CP170719
Coordinates   1362795..1363409 (-) Length   204 a.a.
NCBI ID   WP_064107157.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain JP5008     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1357795..1368409
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFW98_RS06205 (ACFW98_06205) rpsT 1358098..1358364 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  ACFW98_RS06210 (ACFW98_06210) holA 1358381..1359422 (-) 1042 Protein_1225 DNA polymerase III subunit delta -
  ACFW98_RS06215 (ACFW98_06215) - 1359462..1359613 (-) 152 Protein_1226 hypothetical protein -
  ACFW98_RS06220 (ACFW98_06220) - 1359654..1359788 (+) 135 WP_003152870.1 YqzM family protein -
  ACFW98_RS06225 (ACFW98_06225) comEC 1359807..1362158 (-) 2352 WP_395096884.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  ACFW98_RS06230 (ACFW98_06230) - 1362159..1362728 (-) 570 WP_003152868.1 ComE operon protein 2 -
  ACFW98_RS06235 (ACFW98_06235) comEA 1362795..1363409 (-) 615 WP_064107157.1 helix-hairpin-helix domain-containing protein Machinery gene
  ACFW98_RS06240 (ACFW98_06240) comER 1363468..1364289 (+) 822 WP_242750419.1 late competence protein ComER -
  ACFW98_RS06245 (ACFW98_06245) - 1364358..1365095 (-) 738 WP_395096885.1 class I SAM-dependent DNA methyltransferase -
  ACFW98_RS06250 (ACFW98_06250) rsfS 1365092..1365448 (-) 357 WP_007408260.1 ribosome silencing factor -
  ACFW98_RS06255 (ACFW98_06255) yqeK 1365466..1366026 (-) 561 WP_014418422.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  ACFW98_RS06260 (ACFW98_06260) - 1366016..1366585 (-) 570 WP_007408258.1 nicotinate-nucleotide adenylyltransferase -
  ACFW98_RS06265 (ACFW98_06265) yhbY 1366596..1366886 (-) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -
  ACFW98_RS06270 (ACFW98_06270) aroE 1366880..1367722 (-) 843 WP_033574150.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22249.47 Da        Isoelectric Point: 6.4888

>NTDB_id=1058724 ACFW98_RS06235 WP_064107157.1 1362795..1363409(-) (comEA) [Bacillus amyloliquefaciens strain JP5008]
MTKAMMTERLRRNLKILVACLAAGAIFICIWLFRSEKEEPVKQQSVLGQTASVSVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=1058724 ACFW98_RS06235 WP_064107157.1 1362795..1363409(-) (comEA) [Bacillus amyloliquefaciens strain JP5008]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATCCTGGTCGCTTGTCTTGCGGCAGGGGCGATTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTTGGCCAAACAGCCTCAGTAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(68-122)

Helix-hairpin-helix motif (HHH_3)

(141-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

57.653

96.078

0.554
  comEA Lactococcus lactis subsp. cremoris KW2

35.135

100

0.382
  comEA Latilactobacillus sakei subsp. sakei 23K

33.19

100

0.377
  comEA/celA/cilE Streptococcus pneumoniae Rx1

36.493

100

0.377
  comEA/celA/cilE Streptococcus pneumoniae D39

36.493

100

0.377
  comEA/celA/cilE Streptococcus pneumoniae R6

36.493

100

0.377
  comEA/celA/cilE Streptococcus mitis NCTC 12261

36.493

100

0.377
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

36.493

100

0.377
  comEA/celA/cilE Streptococcus mitis SK321

35.071

100

0.363

Multiple sequence alignment