Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEC   Type   Machinery gene
Locus tag   ACFW98_RS06225 Genome accession   NZ_CP170719
Coordinates   1359807..1362158 (-) Length   783 a.a.
NCBI ID   WP_395096884.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain JP5008     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1354807..1367158
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFW98_RS06190 (ACFW98_06190) - 1355173..1355511 (-) 339 WP_007408270.1 YqxA family protein -
  ACFW98_RS06195 (ACFW98_06195) spoIIP 1355528..1356721 (-) 1194 WP_113766757.1 stage II sporulation protein P -
  ACFW98_RS06200 (ACFW98_06200) gpr 1356789..1357895 (-) 1107 WP_007408268.1 GPR endopeptidase -
  ACFW98_RS06205 (ACFW98_06205) rpsT 1358098..1358364 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  ACFW98_RS06210 (ACFW98_06210) holA 1358381..1359422 (-) 1042 Protein_1225 DNA polymerase III subunit delta -
  ACFW98_RS06215 (ACFW98_06215) - 1359462..1359613 (-) 152 Protein_1226 hypothetical protein -
  ACFW98_RS06220 (ACFW98_06220) - 1359654..1359788 (+) 135 WP_003152870.1 YqzM family protein -
  ACFW98_RS06225 (ACFW98_06225) comEC 1359807..1362158 (-) 2352 WP_395096884.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  ACFW98_RS06230 (ACFW98_06230) - 1362159..1362728 (-) 570 WP_003152868.1 ComE operon protein 2 -
  ACFW98_RS06235 (ACFW98_06235) comEA 1362795..1363409 (-) 615 WP_064107157.1 helix-hairpin-helix domain-containing protein Machinery gene
  ACFW98_RS06240 (ACFW98_06240) comER 1363468..1364289 (+) 822 WP_242750419.1 late competence protein ComER -
  ACFW98_RS06245 (ACFW98_06245) - 1364358..1365095 (-) 738 WP_395096885.1 class I SAM-dependent DNA methyltransferase -
  ACFW98_RS06250 (ACFW98_06250) rsfS 1365092..1365448 (-) 357 WP_007408260.1 ribosome silencing factor -
  ACFW98_RS06255 (ACFW98_06255) yqeK 1365466..1366026 (-) 561 WP_014418422.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  ACFW98_RS06260 (ACFW98_06260) - 1366016..1366585 (-) 570 WP_007408258.1 nicotinate-nucleotide adenylyltransferase -
  ACFW98_RS06265 (ACFW98_06265) yhbY 1366596..1366886 (-) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -

Sequence

Protein


Download         Length: 783 a.a.        Molecular weight: 86655.39 Da        Isoelectric Point: 8.7893

>NTDB_id=1058722 ACFW98_RS06225 WP_395096884.1 1359807..1362158(-) (comEC) [Bacillus amyloliquefaciens strain JP5008]
MKYKYLLLPLAAVSATAGIAAAHVFWVLLLFLLYLLFIIVKTKHHAPVIVCLVSFCLYFYLYTVCDAANVTRYQAGSYTE
QAVITNIPKVDGAKMSAVIRTHDKEKWAASYKIRSLEEKRLIEQLEPGMRCTFTGSLEQPAHATVPGGFDYKEYLYSQQI
HWLFSVTSIEQCEKSKQPLFKLLNIRKNLISIIRNHVPESSAGIVEALTLGERFSIEDDILSAYQNLGVVHLMAISGMHV
GLITAGLFYALIRIGLTREKAGILLLLFLPVYTLLSGAAPSVLRASLMLGFYIAGTLVKRGIHSSAALSLSYLLLLLFNP
YFLWQAGFQLSFAVSASLILSSSILKKAGESRLAGLAMASFIAELSSLPFLLYHFQQISLVSFPMNMVMVPFYTLFVIPV
SVIGFLLLLLSRQMGECLFGMFDLVMKPVHDFITYAASVDLFTMIVSKPDFVSLLLLAVSVFTLFAALEKGGFLKLRKSA
LFFCAVLAYLICRPYFSPWGEADMLDIGQGDSLFISAPHRKGTVMVDTGGVIAYPGESWKEKRHPYSIGEKVLIPFLNGK
GVKKLDALILTHADQDHIGEAGVLIKNHRVKRLIVPVGFVKEPKDQNILNMAKENNIPVAEAKRGDTITAGDLQFQVLSP
ESSDGRSKNDSSLVLWTVFGGVSWLLTGDLESDGETEVLKTYPNLKADILKAGHHGSKSSTSEGFLKQLQPEAALISAGK
ENRYHHPHEEVLDRLKAYSVNVLRTDVSGTIQYRFKKGAGTFSVFPPYDIEETRAQEVKKTAD

Nucleotide


Download         Length: 2352 bp        

>NTDB_id=1058722 ACFW98_RS06225 WP_395096884.1 1359807..1362158(-) (comEC) [Bacillus amyloliquefaciens strain JP5008]
ATGAAATATAAATACCTTCTTCTGCCTCTGGCGGCGGTTTCTGCAACTGCGGGAATTGCCGCCGCTCATGTCTTCTGGGT
TCTGCTCCTTTTTCTTCTGTATCTTCTCTTTATTATTGTAAAAACAAAGCACCATGCTCCGGTTATCGTCTGCCTCGTTT
CTTTTTGTCTTTATTTCTATCTTTATACGGTTTGTGACGCTGCGAATGTAACGCGCTATCAGGCCGGCAGTTATACTGAA
CAGGCCGTCATCACTAATATTCCGAAGGTTGACGGAGCGAAAATGTCAGCTGTTATCCGTACACATGACAAGGAAAAATG
GGCGGCTTCGTACAAAATCCGTTCTCTTGAGGAAAAAAGACTCATTGAACAGCTTGAACCGGGGATGCGTTGCACGTTTA
CAGGCTCTCTGGAACAGCCTGCACATGCGACGGTTCCCGGAGGTTTTGATTATAAGGAATATCTTTACTCTCAGCAGATT
CACTGGTTATTTTCCGTGACTTCCATTGAGCAGTGTGAAAAATCCAAACAGCCGCTGTTTAAACTGCTGAACATCAGAAA
AAATTTGATTTCGATCATTCGGAATCACGTGCCTGAATCTTCCGCCGGAATTGTTGAAGCGCTGACCTTAGGTGAAAGAT
TTTCTATAGAGGACGATATACTGAGTGCATATCAAAATTTGGGAGTCGTTCATTTAATGGCGATTTCCGGAATGCATGTC
GGTCTTATTACGGCGGGATTATTTTATGCTCTGATCAGAATCGGGCTGACAAGAGAAAAGGCGGGAATTTTGCTGCTGCT
GTTTTTGCCGGTGTATACGCTGCTGAGCGGTGCCGCCCCATCCGTATTGCGCGCATCCCTCATGCTGGGATTTTATATCG
CCGGAACTCTTGTTAAACGCGGCATTCATTCCTCTGCTGCATTGTCCTTGTCTTATCTGCTGCTTCTGCTGTTTAATCCT
TACTTCCTTTGGCAGGCGGGCTTCCAGCTTTCCTTTGCGGTAAGCGCCTCTTTAATTCTGTCATCCTCCATTTTAAAGAA
AGCAGGGGAAAGCAGACTTGCCGGGCTTGCGATGGCTTCATTCATTGCGGAGCTCAGCTCACTTCCGTTTCTTCTCTATC
ATTTTCAGCAGATTTCACTTGTCAGTTTTCCGATGAATATGGTGATGGTGCCTTTTTATACGTTATTTGTCATTCCGGTT
TCTGTCATCGGTTTCCTTCTTCTTTTACTCTCAAGGCAGATGGGAGAATGTTTGTTTGGTATGTTTGACCTTGTGATGAA
GCCTGTGCATGATTTCATTACATATGCGGCATCCGTTGATTTATTTACTATGATTGTGTCAAAGCCTGACTTTGTTTCCC
TTCTTCTGCTTGCGGTTTCCGTTTTTACGCTTTTTGCGGCTTTGGAAAAGGGAGGTTTTTTAAAACTCAGGAAATCGGCT
CTTTTTTTCTGCGCGGTTTTGGCTTATTTAATATGCCGTCCGTATTTCAGTCCATGGGGAGAAGCGGATATGCTTGATAT
CGGGCAGGGAGACTCACTGTTTATAAGCGCGCCGCACCGCAAAGGGACCGTAATGGTTGATACAGGGGGAGTGATTGCTT
ATCCCGGAGAATCATGGAAAGAAAAACGCCACCCATATTCTATCGGCGAGAAGGTTTTGATTCCATTTTTAAACGGAAAA
GGGGTGAAAAAGCTGGATGCGCTGATTTTAACCCATGCGGATCAAGATCACATCGGGGAAGCCGGAGTGTTAATCAAAAA
TCATAGAGTCAAACGGTTAATTGTCCCCGTAGGATTCGTAAAAGAACCGAAAGATCAGAACATATTAAATATGGCGAAAG
AAAACAACATTCCCGTTGCCGAAGCAAAGCGGGGCGACACCATTACAGCCGGGGATCTTCAGTTTCAGGTGCTGTCTCCG
GAGTCGTCTGACGGAAGGAGTAAAAATGATTCGTCACTGGTGCTTTGGACGGTTTTTGGCGGAGTGAGCTGGCTTTTGAC
GGGAGATTTAGAATCGGACGGCGAAACGGAAGTGCTGAAAACGTATCCGAATCTGAAGGCTGATATATTGAAGGCGGGTC
ATCACGGCAGCAAAAGCTCAACGAGTGAAGGCTTTTTGAAGCAGCTTCAGCCGGAAGCAGCGCTGATTTCAGCAGGAAAA
GAGAATCGATACCATCATCCGCATGAAGAAGTGCTGGATCGTTTGAAGGCGTACTCTGTCAATGTGCTTCGCACCGATGT
CAGCGGAACGATTCAATACAGATTTAAAAAAGGCGCCGGAACGTTTTCCGTCTTCCCTCCATATGATATAGAAGAAACCA
GGGCGCAAGAAGTAAAAAAGACTGCCGATTGA

Domains

Predicted by InterproScan.

Metallo-beta-lactamase superfamily (Lactamase_B)

(508-719)

Domain of unknown function (DUF4131) (DUF4131)

(25-171)

Competence protein (Competence)

(208-469)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEC Bacillus subtilis subsp. subtilis str. 168

57.254

98.595

0.564

Multiple sequence alignment