Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   AAA982_RS09605 Genome accession   NZ_AP028601
Coordinates   1850018..1851034 (-) Length   338 a.a.
NCBI ID   WP_138028521.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain PF1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1845018..1856034
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAA982_RS09560 - 1845096..1845227 (-) 132 WP_000768904.1 hypothetical protein -
  AAA982_RS09565 (TKY113292_18610) - 1845228..1846418 (-) 1191 WP_050085692.1 acetate kinase -
  AAA982_RS09570 (TKY113292_18620) comYH 1846469..1847422 (-) 954 WP_044813830.1 class I SAM-dependent methyltransferase Machinery gene
  AAA982_RS09575 - 1847483..1848077 (-) 595 Protein_1860 methyltransferase domain-containing protein -
  AAA982_RS09580 (TKY113292_18650) comGG/cglG 1848180..1848626 (-) 447 WP_001811371.1 competence type IV pilus minor pilin ComGG Machinery gene
  AAA982_RS09585 comGF/cglF 1848604..1849065 (-) 462 WP_000250539.1 competence type IV pilus minor pilin ComGF Machinery gene
  AAA982_RS09590 (TKY113292_18670) comGE/cglE 1849028..1849330 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  AAA982_RS09595 (TKY113292_18680) comGD/cglD 1849293..1849697 (-) 405 WP_000588030.1 competence type IV pilus minor pilin ComGD Machinery gene
  AAA982_RS09600 (TKY113292_18690) comGC/cglC 1849690..1850016 (-) 327 WP_000738625.1 competence type IV pilus major pilin ComGC Machinery gene
  AAA982_RS09605 (TKY113292_18700) comGB/cglB 1850018..1851034 (-) 1017 WP_138028521.1 competence type IV pilus assembly protein ComGB Machinery gene
  AAA982_RS09610 (TKY113292_18710) comGA/cglA/cilD 1850982..1851923 (-) 942 WP_016398028.1 competence type IV pilus ATPase ComGA Machinery gene
  AAA982_RS09615 (TKY113292_18720) - 1851999..1852364 (-) 366 WP_000286415.1 DUF1033 family protein -
  AAA982_RS09620 (TKY113292_18730) - 1852515..1853573 (-) 1059 WP_000649473.1 zinc-dependent alcohol dehydrogenase family protein -
  AAA982_RS09625 (TKY113292_18740) nagA 1853736..1854887 (-) 1152 WP_001134454.1 N-acetylglucosamine-6-phosphate deacetylase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38585.65 Da        Isoelectric Point: 9.7316

>NTDB_id=105844 AAA982_RS09605 WP_138028521.1 1850018..1851034(-) (comGB/cglB) [Streptococcus pneumoniae strain PF1]
MDISQVFRLRRKKLATAKQKNIITLFNNFFSSGFHLVETISFLDRSALLDKQCVTRMRVGLSQGKSFSEMMESLGCSSAI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLFFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
LGMAGLVSVLALLALTFYKRSSKMNVFSILARLPFIGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEI
GQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=105844 AAA982_RS09605 WP_138028521.1 1850018..1851034(-) (comGB/cglB) [Streptococcus pneumoniae strain PF1]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATTTCTTTTCTAGCGGTTTTCATCTGGTGGAGACCATCTCCTTTTTAGATAGGAGTGCTTTGTTGGACAAGCAGTGTG
TGACCCGGATGCGTGTGGGCTTGTCTCAGGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGATGTTCAAGTGCTATT
GTCACTCAGTTATCCCTAGCTGAAGTTCATGGCAATCTCCACCTGAGTTTGGGAAAGATAGAAGAATATCTGGACAATCT
GGCCAAGGTCAAGAAAAAATTGATTGAAGTAGCGACCTATCCCTTGATTTTGCTGTTTTTTCTTCTCTTAATCATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCGAATTATCGGTAATCTGCCCCAAATTTTT
CTAGGCATGGCAGGGCTTGTTTCCGTGCTTGCCCTTTTAGCACTAACTTTTTATAAAAGAAGTTCTAAGATGAATGTCTT
TTCTATCTTAGCACGCCTTCCCTTTATTGGAATCTTTGTGCAGACCTATTTGACAGCCTATTATGCACGTGAATGGGGGA
ATATGATTTCACAGGGAATGGAGTTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAATC
GGTCAAGATCTGGCTCAAACCCTGAAAAATGGCCGTGAATTTTCTCAGACGATAGGAACCTATCCTTTCTTTAGGAAGGA
ATTGAGTCTCATCATAGAGTATGGGGAAGTTAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATTTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(28-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae Rx1

97.929

100

0.979
  comGB/cglB Streptococcus pneumoniae D39

97.929

100

0.979
  comGB/cglB Streptococcus pneumoniae R6

97.929

100

0.979
  comGB/cglB Streptococcus pneumoniae TIGR4

97.929

100

0.979
  comGB/cglB Streptococcus mitis SK321

94.083

100

0.941
  comGB/cglB Streptococcus mitis NCTC 12261

93.491

100

0.935
  comYB Streptococcus gordonii str. Challis substr. CH1

69.94

99.408

0.695
  comYB Streptococcus mutans UA140

57.729

93.787

0.541
  comYB Streptococcus mutans UA159

57.729

93.787

0.541
  comGB Lactococcus lactis subsp. cremoris KW2

50.599

98.817

0.5

Multiple sequence alignment