Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACFU5E_RS07875 Genome accession   NZ_CP170577
Coordinates   1692023..1693162 (-) Length   379 a.a.
NCBI ID   WP_378081755.1    Uniprot ID   -
Organism   Aeromonas bestiarum strain S1-4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1687023..1698162
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFU5E_RS07850 (ACFU5E_07850) - 1687024..1687251 (+) 228 WP_042866070.1 FeoA family protein -
  ACFU5E_RS07855 (ACFU5E_07855) feoB 1687248..1689518 (+) 2271 WP_378081753.1 Fe(2+) transporter permease subunit FeoB -
  ACFU5E_RS07860 (ACFU5E_07860) - 1689515..1689757 (+) 243 WP_043140913.1 FeoC-like transcriptional regulator -
  ACFU5E_RS07865 (ACFU5E_07865) - 1689859..1690338 (-) 480 WP_290019569.1 WbuC family cupin fold metalloprotein -
  ACFU5E_RS07870 (ACFU5E_07870) glgC 1690478..1691749 (-) 1272 WP_043139205.1 glucose-1-phosphate adenylyltransferase -
  ACFU5E_RS07875 (ACFU5E_07875) pilU 1692023..1693162 (-) 1140 WP_378081755.1 type IVa pilus ATPase TapW Machinery gene
  ACFU5E_RS07880 (ACFU5E_07880) - 1693222..1693578 (-) 357 WP_043139210.1 DMT family protein -
  ACFU5E_RS07885 (ACFU5E_07885) - 1693692..1694423 (+) 732 WP_043556548.1 ABC transporter substrate-binding protein -
  ACFU5E_RS07890 (ACFU5E_07890) btuC 1694527..1695534 (+) 1008 WP_098983659.1 vitamin B12 ABC transporter permease BtuC -
  ACFU5E_RS07895 (ACFU5E_07895) - 1695594..1696403 (+) 810 WP_378081757.1 ATP-binding cassette domain-containing protein -
  ACFU5E_RS07900 (ACFU5E_07900) aac(6') 1696426..1696887 (-) 462 WP_043556544.1 aminoglycoside 6'-N-acetyltransferase -
  ACFU5E_RS07905 (ACFU5E_07905) - 1696898..1697962 (-) 1065 WP_043139222.1 3-deoxy-7-phosphoheptulonate synthase -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42317.47 Da        Isoelectric Point: 6.2570

>NTDB_id=1058090 ACFU5E_RS07875 WP_378081755.1 1692023..1693162(-) (pilU) [Aeromonas bestiarum strain S1-4]
MELRDMLQILAQQDGSDLYLSTGAPPCAKFNGGLRPLLETPLEPGEVAKIAADIMDGEQKLQFERELEMNLAISLPQIGR
FRINIFKQRNEVSLVARNIKTEIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAAVEIMLGTHTIRDMIKRGEFGGLKEVMEKSRALGMVTFDSAL
FDLVVEGVIEEEEAVKNADSANNLRLKIKLWKEKGQIASSSDATGWSLEPTKDEKDELF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=1058090 ACFU5E_RS07875 WP_378081755.1 1692023..1693162(-) (pilU) [Aeromonas bestiarum strain S1-4]
ATGGAATTGCGAGACATGCTACAGATCCTGGCCCAGCAGGACGGCTCCGACCTCTATCTGTCGACCGGCGCCCCCCCCTG
CGCCAAGTTCAATGGCGGATTGCGTCCCCTGCTGGAGACGCCGCTCGAGCCGGGCGAGGTGGCGAAGATTGCCGCCGACA
TCATGGACGGCGAGCAGAAGCTGCAGTTCGAACGGGAGCTGGAGATGAACCTCGCCATCTCCTTGCCCCAGATAGGGCGC
TTTCGGATCAACATCTTCAAGCAGCGCAACGAAGTGTCACTGGTGGCCCGCAACATCAAGACCGAGATCCCCCGCTTCGA
GGATCTGAAACTGCCTCCCGTGCTGCTCGACACCATCATGGAGAAACGCGGTCTGGTGCTGTTCGTCGGGGGAACCGGCT
CGGGCAAGTCCACCTCGCTGGCGGCCCTCATCGACCATCGCAACCGCAACAGCGGCGGCCACATCATCACCATAGAGGAT
CCGGTGGAGTTCGTTCACCGCCACCGCAAGAGCATCATCAACCAGCGGGAGGTGGGGGTGGATACCCGCAGCTTCCACGC
CGCGCTCAAGAATACCCTGCGCCAGGCGCCGGACGTGATCCTCATCGGCGAAATTCGCGACCGCGAAACCATGGAGCATG
CGCTGGCCTTCTCCGAAACCGGCCACCTCGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGGAGGAGCGCCGCCCGCAACTGCTCAACGATCTGGGCAACAACCTCAAGGCCTTTGTCTCCCAACG
GCTGGTCAAAACCACGGACGGCGGCCGCCGGGCGGCGGTGGAGATCATGCTGGGCACCCACACCATCCGCGACATGATCA
AGCGCGGCGAGTTTGGCGGCCTCAAGGAGGTGATGGAGAAATCCAGGGCCCTCGGCATGGTCACCTTCGACAGCGCCCTG
TTCGATCTGGTGGTGGAAGGGGTGATCGAGGAGGAAGAGGCGGTGAAGAATGCCGACTCGGCCAACAACCTGCGCCTCAA
GATCAAGCTGTGGAAGGAGAAGGGCCAGATAGCCAGCAGCAGCGATGCCACGGGTTGGAGTCTGGAGCCCACCAAGGATG
AGAAAGACGAACTCTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

53.684

100

0.538
  pilU Acinetobacter baylyi ADP1

51.955

94.459

0.491
  pilU Vibrio cholerae strain A1552

50

92.348

0.462
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.888

89.446

0.375
  pilT Legionella pneumophila strain ERS1305867

42.216

88.127

0.372
  pilT Legionella pneumophila strain Lp02

42.216

88.127

0.372
  pilT Pseudomonas aeruginosa PAK

40.698

90.765

0.369
  pilT Pseudomonas stutzeri DSM 10701

40.116

90.765

0.364

Multiple sequence alignment