Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACFU5E_RS03295 Genome accession   NZ_CP170577
Coordinates   692728..693837 (-) Length   369 a.a.
NCBI ID   WP_043142397.1    Uniprot ID   A0AAW7HZS2
Organism   Aeromonas bestiarum strain S1-4     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 687728..698837
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFU5E_RS03275 (ACFU5E_03275) brnQ 688171..689478 (-) 1308 WP_290018594.1 branched-chain amino acid transport system II carrier protein -
  ACFU5E_RS03280 (ACFU5E_03280) - 689670..690380 (-) 711 WP_378081230.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  ACFU5E_RS03285 (ACFU5E_03285) srmB 690509..691732 (+) 1224 WP_043142402.1 ATP-dependent RNA helicase SrmB -
  ACFU5E_RS03290 (ACFU5E_03290) yaaA 691923..692696 (-) 774 WP_043142400.1 peroxide stress protein YaaA -
  ACFU5E_RS03295 (ACFU5E_03295) pilU 692728..693837 (-) 1110 WP_043142397.1 type IVa pilus ATPase TapU Machinery gene
  ACFU5E_RS03300 (ACFU5E_03300) pilT 693866..694900 (-) 1035 WP_005320466.1 type IVa pilus ATPase TapT Machinery gene
  ACFU5E_RS03305 (ACFU5E_03305) - 694940..695641 (+) 702 WP_290018599.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACFU5E_RS03310 (ACFU5E_03310) proC 695732..696556 (+) 825 WP_378081233.1 pyrroline-5-carboxylate reductase -
  ACFU5E_RS03315 (ACFU5E_03315) - 696585..697136 (+) 552 WP_043142388.1 YggT family protein -
  ACFU5E_RS03320 (ACFU5E_03320) yggU 697136..697435 (+) 300 WP_103472544.1 DUF167 family protein YggU -
  ACFU5E_RS03325 (ACFU5E_03325) - 697454..697873 (+) 420 WP_043142382.1 DUF4426 domain-containing protein -
  ACFU5E_RS03330 (ACFU5E_03330) - 697943..698242 (-) 300 WP_378081234.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41097.15 Da        Isoelectric Point: 6.4431

>NTDB_id=1058084 ACFU5E_RS03295 WP_043142397.1 692728..693837(-) (pilU) [Aeromonas bestiarum strain S1-4]
MSLDALLTELVARKGSDLFITVGTPPTLKVNGHLQSLGGEPLDKKSALTLVKETLSQDHFERYIRTKEANYAIHREDLGR
FRVSAFWQQDLPGMVLRRIETRIPTFDELTLPPILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGFRNQHADGHILTVED
PVEFVHQHGRSLITQREVGIDTESFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSVDGRRRFAAFEVLLNTPLITDIIRKGEMHRLKEVMTKSTELGMQTFDQA
LFTLFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=1058084 ACFU5E_RS03295 WP_043142397.1 692728..693837(-) (pilU) [Aeromonas bestiarum strain S1-4]
ATGAGTCTGGATGCCCTGTTGACCGAACTGGTCGCACGCAAAGGTTCGGATCTGTTCATCACGGTCGGCACGCCCCCCAC
CCTCAAGGTGAACGGCCATCTGCAGTCCCTGGGGGGAGAGCCGCTCGACAAGAAGAGTGCCCTGACCCTGGTCAAGGAGA
CATTAAGCCAGGATCACTTCGAGCGTTACATCCGCACCAAGGAAGCCAACTACGCCATCCACCGCGAGGATCTTGGCCGC
TTTCGGGTCAGCGCCTTCTGGCAGCAGGATCTGCCGGGCATGGTGTTGCGCCGTATCGAGACCCGGATCCCGACCTTCGA
CGAGCTGACCCTGCCCCCCATCTTGCAGGAGGTGGCCATGGCCAAGCGCGGGCTTGTGCTGTTCGTCGGCGCCACAGGTG
CGGGCAAGTCCACCACCCAGGCCGCCATGATAGGGTTTCGCAACCAGCACGCCGATGGCCATATCCTGACGGTGGAAGAT
CCTGTGGAGTTCGTGCACCAGCACGGCCGCAGCCTGATCACCCAGCGGGAGGTGGGGATAGACACGGAGTCGTTCGACGT
GGCGCTGAAGAGCTCGTTGCGCCAGGCGCCGGACGTGATCCTCATCGGCGAGATCCGCAGCCAGGAGACCATGGAGTTTG
CGCTGCAATTTGCCGAGACGGGTCACCTCTGTCTCGCCACCCTGCACGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTCCACCTGGTACCGCAGGAGAAACACCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTGCGCGCCATAGTGGCCCAGCA
ACTGCTGCCGAGCGTGGACGGTCGCCGCCGTTTTGCGGCCTTCGAGGTGCTGCTCAACACCCCGCTCATCACCGACATCA
TCCGCAAGGGGGAAATGCATCGCCTCAAGGAGGTGATGACCAAGTCCACCGAGCTCGGCATGCAGACCTTCGATCAGGCG
CTGTTCACCCTGTTCTGTGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTGTCAGGCCGTGAACAGCTGGGTGCAGGCACACTCGACAACGTCACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.054

99.729

0.599
  pilU Pseudomonas stutzeri DSM 10701

58.974

95.122

0.561
  pilU Acinetobacter baylyi ADP1

58.074

95.664

0.556
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.9

89.702

0.385
  pilT Legionella pneumophila strain ERS1305867

40.525

92.954

0.377
  pilT Legionella pneumophila strain Lp02

40.525

92.954

0.377
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.12

90.515

0.363
  pilT Vibrio cholerae strain A1552

40.12

90.515

0.363

Multiple sequence alignment