Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   O1Q81_RS09345 Genome accession   NZ_CP170385
Coordinates   1942074..1943522 (-) Length   482 a.a.
NCBI ID   WP_386697288.1    Uniprot ID   -
Organism   Lonepinella sp. MS14436     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1937074..1948522
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O1Q81_RS09310 (O1Q81_01854) - 1937376..1937810 (-) 435 WP_386697271.1 hypothetical protein -
  O1Q81_RS09315 (O1Q81_01855) - 1937908..1938408 (-) 501 WP_386697274.1 hypothetical protein -
  O1Q81_RS09320 (O1Q81_01856) - 1938483..1939205 (-) 723 WP_386697276.1 hypothetical protein -
  O1Q81_RS09325 (O1Q81_01857) - 1939467..1939730 (+) 264 WP_386697278.1 type II toxin-antitoxin system prevent-host-death family antitoxin -
  O1Q81_RS09330 (O1Q81_01858) - 1939730..1940149 (+) 420 WP_386697281.1 type II toxin-antitoxin system VapC family toxin -
  O1Q81_RS09335 (O1Q81_01859) - 1940146..1940826 (-) 681 WP_386697283.1 prepilin peptidase -
  O1Q81_RS09340 (O1Q81_01860) pilC 1940844..1942064 (-) 1221 WP_386697286.1 type II secretion system F family protein Machinery gene
  O1Q81_RS09345 (O1Q81_01861) pilB 1942074..1943522 (-) 1449 WP_386697288.1 GspE/PulE family protein Machinery gene
  O1Q81_RS09350 (O1Q81_01862) pilA 1943522..1944016 (-) 495 WP_386694674.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  O1Q81_RS09355 (O1Q81_01863) ampD 1944161..1944706 (+) 546 WP_386697291.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  O1Q81_RS09360 (O1Q81_01864) - 1944710..1945360 (+) 651 WP_386687262.1 HAD family hydrolase -
  O1Q81_RS09365 (O1Q81_01865) - 1945612..1945881 (+) 270 WP_153984892.1 PTS sugar transporter subunit IIB -
  O1Q81_RS09370 (O1Q81_01866) - 1945891..1947246 (+) 1356 WP_386697294.1 PTS ascorbate transporter subunit IIC -
  O1Q81_RS09375 (O1Q81_01867) - 1947247..1948065 (+) 819 WP_386697296.1 transketolase -

Sequence

Protein


Download         Length: 482 a.a.        Molecular weight: 54424.39 Da        Isoelectric Point: 6.7299

>NTDB_id=1056502 O1Q81_RS09345 WP_386697288.1 1942074..1943522(-) (pilB) [Lonepinella sp. MS14436]
MQKSLSSPHIVSPNVVSPNVVSPNVVSHDGKIFQISPQLWQQNQQQQNILIRYLACPLAENERCLWLGLDSLNNLSACET
FSFLTGKMIEPVLLETQVLKDLLQALAPQSLMVQESPVFYHVQQQDLNEEKHSTQQSDEPVIQLLNRIFESALQQRASDI
HLEPQSQHLQVRFRIDGVLQHQPPVALALSSRVISRLKLLAKLDISETRLPQDGRFHFKTTFSDMLDFRLSTLPTPFGEK
AVLRVQQNKPVQFAFADLGMNDQQQERFKYALNQPQGLILVTGPTGSGKSITLYTALSYLNRPEKHIMTAEDPIEIPLSG
IIQSQVNPQIGLDFSRLLRTFLRQDPDIIMLGEIRDEESAAMALRASQTGHLVLSTLHTNDAPSAIPRLLQLGIKQYEID
NSLLLVIAQRLIRKRCQKCGGKSENSCDCHQGYKGRIGIYQFLQPDPEQCYQLDFDHLQQAGLEKVKQGITDLVEIQRVL
GH

Nucleotide


Download         Length: 1449 bp        

>NTDB_id=1056502 O1Q81_RS09345 WP_386697288.1 1942074..1943522(-) (pilB) [Lonepinella sp. MS14436]
ATGCAAAAGAGTCTATCTAGTCCACATATTGTTAGCCCGAATGTTGTTAGCCCGAATGTTGTTAGCCCGAATGTGGTGAG
CCATGACGGAAAAATATTCCAAATTTCACCGCAGTTATGGCAACAAAATCAGCAACAACAGAACATTCTGATCCGTTATT
TAGCTTGTCCATTAGCGGAAAATGAGCGTTGTTTATGGCTCGGGCTAGATAGCCTCAACAATTTATCCGCGTGTGAAACC
TTTTCTTTCCTAACAGGAAAAATGATCGAACCTGTGTTGTTGGAAACACAGGTATTGAAAGATTTATTGCAAGCCTTAGC
TCCACAATCTTTAATGGTGCAAGAAAGCCCTGTTTTTTACCATGTTCAACAGCAAGATTTAAACGAAGAAAAGCATTCTA
CTCAACAATCAGATGAACCCGTGATTCAGTTGCTTAATCGGATTTTTGAAAGTGCGTTGCAGCAACGAGCATCTGATATT
CACCTTGAGCCACAAAGCCAACATTTGCAAGTGCGTTTTCGTATTGATGGTGTATTACAACACCAACCACCAGTTGCGTT
GGCATTATCTAGCAGGGTGATTTCACGTTTAAAATTATTAGCCAAACTAGATATTAGCGAAACTCGCTTGCCGCAAGACG
GACGTTTTCATTTTAAAACCACCTTTTCTGATATGTTAGATTTTCGTCTTTCCACCTTACCAACCCCATTCGGGGAAAAG
GCAGTGTTGCGGGTGCAACAAAATAAGCCAGTACAATTTGCATTTGCTGATCTGGGTATGAATGATCAGCAGCAGGAACG
TTTTAAATACGCCCTTAATCAACCGCAAGGCTTGATTTTAGTGACAGGACCTACGGGAAGTGGGAAAAGTATCACACTTT
ACACCGCACTTTCTTATTTAAATCGTCCTGAAAAACATATTATGACCGCGGAAGATCCTATTGAAATTCCATTGTCTGGC
ATTATTCAAAGCCAAGTCAATCCACAAATTGGGTTAGATTTTAGTCGGTTATTGCGAACCTTTCTCCGTCAAGATCCCGA
TATTATTATGCTCGGTGAAATTCGTGATGAAGAAAGTGCCGCAATGGCATTACGAGCTTCTCAAACGGGACATCTCGTGC
TTTCCACGTTGCATACCAATGACGCACCGTCCGCTATTCCACGCTTGTTACAGTTAGGTATAAAGCAATACGAAATTGAC
AACAGCTTATTGTTAGTGATTGCACAACGTCTGATTCGGAAACGTTGCCAAAAGTGCGGTGGAAAAAGCGAAAATTCTTG
TGATTGTCATCAAGGCTATAAAGGTCGAATCGGGATTTATCAATTTTTACAACCTGATCCAGAGCAATGTTATCAGTTGG
ATTTTGATCATTTACAACAAGCAGGGCTAGAGAAAGTGAAGCAAGGAATAACGGATTTAGTGGAAATTCAACGAGTATTA
GGACATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(147-414)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

69.803

94.813

0.662
  pilB Haemophilus influenzae Rd KW20

69.584

94.813

0.66
  pilB Glaesserella parasuis strain SC1401

59.348

95.436

0.566
  pilB Vibrio parahaemolyticus RIMD 2210633

39.961

100

0.429
  pilB Vibrio campbellii strain DS40M4

41.529

100

0.417
  pilB Acinetobacter baylyi ADP1

38.71

100

0.398
  pilB Legionella pneumophila strain ERS1305867

39.184

100

0.398
  pilB Vibrio cholerae strain A1552

39.256

100

0.394
  pilB Acinetobacter baumannii D1279779

38.398

100

0.388
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.875

99.585

0.367

Multiple sequence alignment