Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   O1Q81_RS09350 Genome accession   NZ_CP170385
Coordinates   1943522..1944016 (-) Length   164 a.a.
NCBI ID   WP_386694674.1    Uniprot ID   -
Organism   Lonepinella sp. MS14436     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1938522..1949016
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O1Q81_RS09325 (O1Q81_01857) - 1939467..1939730 (+) 264 WP_386697278.1 type II toxin-antitoxin system prevent-host-death family antitoxin -
  O1Q81_RS09330 (O1Q81_01858) - 1939730..1940149 (+) 420 WP_386697281.1 type II toxin-antitoxin system VapC family toxin -
  O1Q81_RS09335 (O1Q81_01859) - 1940146..1940826 (-) 681 WP_386697283.1 prepilin peptidase -
  O1Q81_RS09340 (O1Q81_01860) pilC 1940844..1942064 (-) 1221 WP_386697286.1 type II secretion system F family protein Machinery gene
  O1Q81_RS09345 (O1Q81_01861) pilB 1942074..1943522 (-) 1449 WP_386697288.1 GspE/PulE family protein Machinery gene
  O1Q81_RS09350 (O1Q81_01862) pilA 1943522..1944016 (-) 495 WP_386694674.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  O1Q81_RS09355 (O1Q81_01863) ampD 1944161..1944706 (+) 546 WP_386697291.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  O1Q81_RS09360 (O1Q81_01864) - 1944710..1945360 (+) 651 WP_386687262.1 HAD family hydrolase -
  O1Q81_RS09365 (O1Q81_01865) - 1945612..1945881 (+) 270 WP_153984892.1 PTS sugar transporter subunit IIB -
  O1Q81_RS09370 (O1Q81_01866) - 1945891..1947246 (+) 1356 WP_386697294.1 PTS ascorbate transporter subunit IIC -
  O1Q81_RS09375 (O1Q81_01867) - 1947247..1948065 (+) 819 WP_386697296.1 transketolase -
  O1Q81_RS09380 (O1Q81_01868) - 1948062..1949006 (+) 945 WP_386697298.1 transketolase family protein -

Sequence

Protein


Download         Length: 164 a.a.        Molecular weight: 17219.52 Da        Isoelectric Point: 8.4488

>NTDB_id=1056503 O1Q81_RS09350 WP_386694674.1 1943522..1944016(-) (pilA) [Lonepinella sp. MS14436]
MYFNRTLIQTSYNLQFKKGFTLIELMIVIAIVAILATIAIPSYQNYTKKAAISELLQAAAPYRSEVELCVYNLDKPDNCS
AGQNGIQANITDGSGKYVKSVAVSAGKITVTGKGSVADVSYTLTASGSASTGVTWAANCGTKSELFPAGFCSTNSTSSTS
TGQQ

Nucleotide


Download         Length: 495 bp        

>NTDB_id=1056503 O1Q81_RS09350 WP_386694674.1 1943522..1944016(-) (pilA) [Lonepinella sp. MS14436]
ATGTATTTCAACCGCACTTTGATTCAAACTTCGTACAATCTACAGTTCAAAAAAGGCTTTACCCTGATTGAATTGATGAT
CGTGATTGCCATTGTGGCAATTTTAGCCACCATTGCAATTCCATCTTATCAAAATTATACCAAAAAAGCCGCCATTTCTG
AACTGTTGCAAGCAGCAGCACCTTATCGTTCCGAAGTAGAGCTTTGTGTTTATAACTTAGATAAACCAGATAATTGCTCC
GCAGGTCAAAATGGGATCCAAGCAAATATCACAGATGGTTCAGGCAAATATGTGAAATCTGTTGCCGTCAGTGCTGGCAA
GATTACCGTGACGGGTAAAGGTAGTGTTGCTGATGTGAGTTACACTTTAACTGCCAGCGGTTCAGCTAGTACAGGGGTAA
CTTGGGCGGCAAACTGCGGTACAAAATCGGAATTATTTCCAGCTGGTTTCTGTAGCACAAATAGTACAAGTAGTACAAGC
ACTGGACAACAATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(49-149)

Prokaryotic N-terminal methylation motif (N_methyl)

(17-39)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

66.423

83.537

0.555
  pilA Haemophilus influenzae Rd KW20

62.774

83.537

0.524
  pilA Glaesserella parasuis strain SC1401

54.545

87.195

0.476

Multiple sequence alignment