Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AB5G97_RS19395 Genome accession   NZ_CP170371
Coordinates   4134275..4135990 (+) Length   571 a.a.
NCBI ID   WP_323910782.1    Uniprot ID   -
Organism   Aeromonas veronii strain Aer12     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4129275..4140990
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB5G97_RS19370 (AB5G97_19370) pdhR 4130430..4131194 (-) 765 WP_005336127.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  AB5G97_RS19375 (AB5G97_19375) ampD 4131473..4132048 (-) 576 WP_376868698.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  AB5G97_RS19380 (AB5G97_19380) - 4132179..4132649 (+) 471 WP_139709690.1 TIGR02281 family clan AA aspartic protease -
  AB5G97_RS19385 (AB5G97_19385) nadC 4132653..4133528 (+) 876 WP_181269589.1 carboxylating nicotinate-nucleotide diphosphorylase -
  AB5G97_RS19390 (AB5G97_19390) - 4133844..4134284 (+) 441 WP_376868701.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  AB5G97_RS19395 (AB5G97_19395) pilB 4134275..4135990 (+) 1716 WP_323910782.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  AB5G97_RS19400 (AB5G97_19400) pilC 4136231..4137472 (+) 1242 WP_005336136.1 type II secretion system F family protein Machinery gene
  AB5G97_RS19405 (AB5G97_19405) pilD 4137534..4138406 (+) 873 WP_376868705.1 prepilin peptidase Machinery gene
  AB5G97_RS19410 (AB5G97_19410) coaE 4138420..4139034 (+) 615 WP_108541636.1 dephospho-CoA kinase -
  AB5G97_RS19415 (AB5G97_19415) zapD 4139072..4139794 (+) 723 WP_005336141.1 cell division protein ZapD -
  AB5G97_RS19420 (AB5G97_19420) yacG 4139804..4139998 (+) 195 WP_005336142.1 DNA gyrase inhibitor YacG -
  AB5G97_RS19425 (AB5G97_19425) mutT 4140076..4140480 (-) 405 WP_019444787.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62861.32 Da        Isoelectric Point: 5.3170

>NTDB_id=1056373 AB5G97_RS19395 WP_323910782.1 4134275..4135990(+) (pilB) [Aeromonas veronii strain Aer12]
MPIMTSSPNSGLALSLAASSLISEEDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIP
QKYLNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLLESENDALGMEDIDE
SEISDLEVSDDNSRLDESVNTADDDAPIVKYINKILMDAIKRGASDLHFEPYEFKYRVRFRIDGILHEIATPPVNLSNRF
SARLKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALS
KPQGMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGE
IRDLETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLEL
GFTPQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVT
SLAEVNRVTTN

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=1056373 AB5G97_RS19395 WP_323910782.1 4134275..4135990(+) (pilB) [Aeromonas veronii strain Aer12]
TTGCCCATAATGACCTCTAGCCCCAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCTTCACTGATTAGTGAAGAGGACTC
CCAGCGCTATCTGAGCCAGGCCAAGGCCCAGCGCAAACCCTTTGTGACATTTCTGATAGAGAACGAGATCCTCGATAGCA
AGGCGCTCGCCGATTTTTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGATTTGGCCGAGATCCCG
CAGAAGTACCTCAATCAGAAGCTGATTGAGAAACACCACGTCCTGCCCATCTACACCCAGGGCCACACCCTCTATATCGC
CATGTCGGATCCGACCAATGTCTCGGCGTTGGAGGACTTTGGCTTCAGCTTCGGCCTTCATACCGAAGCGCTGCTGGTAG
AGGAGAACAAGCTCACCGCCGCCATCGGCAAGCTGCTGGAGAGCGAAAACGACGCCCTCGGCATGGAGGATATCGACGAG
TCCGAGATTTCGGATCTCGAGGTCTCCGACGATAACAGCCGCCTCGACGAAAGCGTCAATACCGCCGATGACGATGCACC
CATCGTCAAGTACATCAACAAGATCCTGATGGATGCCATCAAGCGCGGTGCCTCTGACCTGCACTTCGAGCCCTATGAGT
TCAAGTATCGGGTGCGCTTTCGCATCGACGGCATTCTGCATGAAATTGCTACGCCGCCGGTCAACCTCTCCAACCGCTTC
TCCGCCCGCCTCAAGGTCATGGCACGACTCGATATCGCCGAGCGGCGGCTGCCACAGGATGGCCGCATCAAGTTGAAGCT
GTCGCGCAACAAGTCGATGGATATGCGGGTCAACACCTTGCCCACCATGTGGGGGGAGAAGATCGTTATCCGTCTGCTCG
ACTCCTCGGCGGCACGCCTCAATATCGAGCAACTCGGCTTTGATGACCGGCAGAAGGCGCAGTACCTGCGCGCCCTCTCC
AAACCGCAGGGTATGATACTGGTCACCGGCCCTACCGGCTCGGGCAAAACGGTCTCGCTCTATACCGGCCTCAATATTCT
CAACACCACCGAGGTCAATATCTCTACCGCCGAAGATCCGGTGGAGATCAACCTGCCCGGCGTCAATCAGGTGCAGGTCA
ACCCCAAGGCGGGGCTCACCTTTGCCAGCGCCTTGCGCTCCTTCCTGCGGCAAGATCCGGATGTGGTGATGGTGGGGGAA
ATACGGGATCTGGAAACTGCCGAAATCGCCATCAAGGCGGCCCAGACCGGCCACTTGGTGCTCTCCACCCTGCATACCAA
CTCCGCCGCTGAGACCCTGACCCGGATGATGAACATGGGAGTTCCGGCCTTCAACATCGCCTCCTCGGTCACCCTGATCA
TGGCCCAGCGGTTGGCCCGTAAACTGTGCGACCACTGCAAGGCGCCGGAAGTGGTGCCGGAAGCCGAACTGCTGGAGCTG
GGCTTTACGCCGCAGCAGCTGGCAGCCGGACTGCGACTGTTCAAACCGGTCGGTTGCAAGGAGTGTTCCGGCGGCTACAA
GGGGCGGGTCGGCATCTACGAAATCATGTTGATGTCGGAGAACATCGCCAAATTGATCATGCAGGGCGCAAACTCCCTGC
AAATCGCCGCTATCGCGCAGAAGGAGGGGATGCGCACCCTGCGCACCTCGGGACTGGAGAAGGCCCGGCTCGGGGTCACC
AGTCTGGCCGAAGTCAACCGGGTCACCACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-149)

Type II/IV secretion system protein (T2SSE)

(194-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.098

100

0.564
  pilB Acinetobacter baylyi ADP1

55.672

100

0.559
  pilB Legionella pneumophila strain ERS1305867

56.722

95.096

0.539
  pilB Vibrio cholerae strain A1552

54.937

97.548

0.536
  pilB Vibrio parahaemolyticus RIMD 2210633

51.681

98.949

0.511
  pilB Vibrio campbellii strain DS40M4

51.241

98.774

0.506
  pilF Neisseria gonorrhoeae MS11

46.631

98.774

0.461
  pilB/pilB1 Synechocystis sp. PCC 6803

39.241

96.848

0.38
  pilF Thermus thermophilus HB27

40.53

92.469

0.375

Multiple sequence alignment