Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LMH68_RS10710 Genome accession   NZ_CP170175
Coordinates   2286209..2287894 (-) Length   561 a.a.
NCBI ID   WP_229647629.1    Uniprot ID   -
Organism   Vibrio sp. F13 strain 10N.222.55.B3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2281209..2292894
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LMH68_RS10680 (LMH68_010680) rplS 2281699..2282052 (+) 354 WP_004735514.1 50S ribosomal protein L19 -
  LMH68_RS10685 (LMH68_010685) yacG 2282364..2282561 (-) 198 WP_004735515.1 DNA gyrase inhibitor YacG -
  LMH68_RS10690 (LMH68_010690) zapD 2282643..2283383 (-) 741 WP_017063237.1 cell division protein ZapD -
  LMH68_RS10695 (LMH68_010695) coaE 2283410..2284024 (-) 615 WP_229647627.1 dephospho-CoA kinase -
  LMH68_RS10700 (LMH68_010700) pilD 2284027..2284896 (-) 870 WP_229647628.1 prepilin peptidase Machinery gene
  LMH68_RS10705 (LMH68_010705) pilC 2284964..2286193 (-) 1230 WP_017065898.1 type II secretion system F family protein Machinery gene
  LMH68_RS10710 (LMH68_010710) pilB 2286209..2287894 (-) 1686 WP_229647629.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LMH68_RS10715 (LMH68_010715) - 2287901..2288356 (-) 456 WP_229647630.1 pilin -
  LMH68_RS10720 (LMH68_010720) nadC 2288610..2289500 (-) 891 WP_017065901.1 carboxylating nicotinate-nucleotide diphosphorylase -
  LMH68_RS10725 (LMH68_010725) ampD 2289584..2290183 (+) 600 WP_229647631.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  LMH68_RS10730 (LMH68_010730) pdhR 2290667..2291428 (+) 762 WP_009847596.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62113.90 Da        Isoelectric Point: 5.3169

>NTDB_id=1055455 LMH68_RS10710 WP_229647629.1 2286209..2287894(-) (pilB) [Vibrio sp. F13 strain 10N.222.55.B3]
MLTNLPTVLRQADLLSLTQEQAVAEHVQASGISTPEALLILKLFTGDSLANNIKTIFGLPLIRLANTDYESLSEQLGLRE
LITKYRAIPISVSSSTLTLASADPTDLQAEDDFRFATGLQVELVVANYSELEGAIRKLYGRSISGQDSKRKEITQDELAN
LVKVSDDEITSIEDLSQDDSPVSRFINQILVDAVRKGASDIHFEPYEENYRVRLRCDGILVEIQQPASHLSRRLSARLKI
LAKLDIAERRLPQDGRIKLRLNDELAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYSEAQKALYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRVLNTTERNISTAEDPVEINLCGINQVQVTPKIGFGFAEALRSFLRQDPDVVMVGEIRDLET
AEIAIKASQTGHLVLSTLHTNSAAETVTRLAHMGIEPFNLASSLSLIIAQRLARRLCNHCKENDDSPDIFLRHSIPNSQT
IYKASPKGCNECNQGYSGRVGIYEVMPFSDQLKSSLIDKPNALAIENLARREGMRTLQESGLDKLLEGTTSYQELQRVLY
I

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=1055455 LMH68_RS10710 WP_229647629.1 2286209..2287894(-) (pilB) [Vibrio sp. F13 strain 10N.222.55.B3]
ATGCTAACCAACCTCCCAACCGTTCTACGTCAGGCTGATTTACTTAGCCTGACTCAGGAACAAGCCGTGGCAGAACATGT
ACAAGCTTCAGGTATTTCGACACCTGAAGCTTTACTCATTCTTAAGTTATTCACAGGCGATTCCTTAGCAAACAACATAA
AAACCATCTTCGGCTTACCGTTAATACGATTAGCCAATACTGACTACGAATCCCTGAGTGAACAATTAGGGCTTCGTGAA
CTGATTACTAAGTATCGTGCTATTCCAATTTCAGTTTCTAGCTCAACTCTCACACTCGCTTCTGCCGACCCTACGGATTT
ACAGGCTGAAGATGACTTCCGCTTTGCGACAGGATTGCAAGTTGAATTGGTTGTCGCTAACTACTCTGAATTAGAAGGTG
CGATACGCAAGCTGTATGGCCGCTCTATTTCCGGGCAAGATTCTAAGCGTAAAGAGATCACCCAAGATGAGCTAGCTAAC
CTCGTCAAGGTATCTGACGACGAAATAACGTCGATAGAAGATCTCAGCCAAGATGACTCTCCTGTGAGTCGCTTTATCAA
TCAGATACTGGTTGATGCGGTGCGTAAAGGCGCATCGGACATCCACTTCGAACCTTATGAAGAAAACTACCGCGTACGTC
TACGTTGCGATGGCATCCTTGTCGAGATCCAACAACCCGCCTCTCATTTAAGCCGTCGTCTGTCAGCTCGCTTAAAGATC
CTCGCTAAACTCGATATTGCCGAACGTCGCTTACCTCAAGATGGCCGTATCAAACTGCGTTTAAATGACGAACTGGCAAT
TGATATGCGCGTATCAACGCTGCCTACCTTATGGGGCGAGAAGATCGTACTGCGATTGCTTGATAGCAGTGCGGCCAATC
TCGATATAGACAAGCTCGGTTACAGCGAGGCTCAAAAAGCCCTCTACCTCAATGCACTAAAGCGCCCGCAAGGGATGATC
TTAATGACCGGGCCAACCGGCAGCGGTAAAACGGTTTCTCTTTATACTGGCCTTCGCGTGCTCAACACTACGGAACGCAA
CATCTCAACAGCCGAAGACCCGGTGGAAATTAACCTGTGTGGTATCAACCAAGTACAAGTAACACCTAAGATCGGCTTTG
GGTTTGCTGAAGCACTGCGATCATTTTTACGACAAGATCCTGATGTTGTGATGGTTGGAGAGATCCGCGACTTGGAAACC
GCCGAAATCGCGATCAAAGCATCGCAAACCGGTCACTTAGTACTTTCGACACTGCACACTAACTCCGCCGCAGAAACCGT
CACTCGACTCGCTCATATGGGGATAGAACCCTTTAACCTCGCTTCATCATTGAGTTTGATTATCGCCCAGCGACTGGCAA
GACGATTATGCAACCACTGCAAAGAAAATGACGACTCGCCAGACATATTCCTGCGTCACTCCATTCCTAACAGCCAAACC
ATCTACAAAGCGAGTCCTAAAGGTTGCAATGAGTGTAACCAGGGTTATTCGGGGCGAGTGGGTATCTATGAAGTGATGCC
GTTTAGCGATCAACTGAAAAGCAGCCTGATCGACAAACCGAACGCGTTGGCCATTGAAAACTTGGCTCGCCGAGAAGGCA
TGAGAACTCTGCAAGAGTCGGGGCTAGATAAATTGCTTGAAGGCACCACCAGCTATCAAGAGCTGCAACGTGTTCTGTAC
ATATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-141)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

73.582

100

0.74
  pilB Vibrio campbellii strain DS40M4

70.641

100

0.708
  pilB Vibrio parahaemolyticus RIMD 2210633

70.641

100

0.708
  pilB Legionella pneumophila strain ERS1305867

47.645

98.396

0.469
  pilB Acinetobacter baumannii D1279779

50.674

92.513

0.469
  pilB Acinetobacter baylyi ADP1

49.326

92.513

0.456
  pilF Neisseria gonorrhoeae MS11

43.964

98.93

0.435
  pilB/pilB1 Synechocystis sp. PCC 6803

34.622

100

0.367
  pilF Thermus thermophilus HB27

38.772

92.87

0.36

Multiple sequence alignment