Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BCS84_RS04230 Genome accession   NZ_CP170046
Coordinates   853050..854738 (-) Length   562 a.a.
NCBI ID   WP_016793486.1    Uniprot ID   -
Organism   Vibrio cyclitrophicus strain 10N.222.45.E8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 848050..859738
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BCS84_RS04200 (BCS84_04200) rplS 848551..848904 (+) 354 WP_004735514.1 50S ribosomal protein L19 -
  BCS84_RS04205 (BCS84_04205) yacG 849207..849404 (-) 198 WP_010435916.1 DNA gyrase inhibitor YacG -
  BCS84_RS04210 (BCS84_04210) zapD 849486..850226 (-) 741 WP_010435917.1 cell division protein ZapD -
  BCS84_RS04215 (BCS84_04215) coaE 850253..850867 (-) 615 WP_016793489.1 dephospho-CoA kinase -
  BCS84_RS04220 (BCS84_04220) pilD 850870..851739 (-) 870 WP_016793488.1 prepilin peptidase Machinery gene
  BCS84_RS04225 (BCS84_04225) pilC 851806..853035 (-) 1230 WP_016793487.1 type II secretion system F family protein Machinery gene
  BCS84_RS04230 (BCS84_04230) pilB 853050..854738 (-) 1689 WP_016793486.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BCS84_RS04235 (BCS84_04235) pilA 854740..855150 (-) 411 WP_016793485.1 pilin Machinery gene
  BCS84_RS04240 (BCS84_04240) nadC 855402..856292 (-) 891 WP_016768207.1 carboxylating nicotinate-nucleotide diphosphorylase -
  BCS84_RS04245 (BCS84_04245) ampD 856376..856975 (+) 600 WP_016791286.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  BCS84_RS04250 (BCS84_04250) pdhR 857457..858218 (+) 762 WP_016768209.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62090.84 Da        Isoelectric Point: 5.3011

>NTDB_id=1054928 BCS84_RS04230 WP_016793486.1 853050..854738(-) (pilB) [Vibrio cyclitrophicus strain 10N.222.45.E8]
MGLTNLPTVLRQAGLLSLAQEQAVAEHVQASGVSTPEALLVLDFFSGDSLANNIKTIFGLPLVQLANTDYETLCDQLGLR
ELITKYRAIPISVSSSTLTLASADPTDLQAEDDFRFATGLQIELVVANYSELEGVIRKLYGRSISGQDSKRKEITQDELA
NLVKVSDDEITSIEDLSQDDSPVSRFINQILVDAVRKGASDIHFEPYEEHYRVRLRCDGILVEIQQPASHLSRRLSARLK
ILAKLDIAERRLPQDGRIKLRLNDELAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYSEAQKALYLNALKRPQGM
ILMTGPTGSGKTVSLYTGLRVLNTSERNISTAEDPVEINLCGINQVQVTPKIGFGFAEALRSFLRQDPDIVMVGEIRDLE
TAEIAIKASQTGHLVLSTLHTNSAAETVTRLAHMGIEPFNLASSLSLIIAQRLARRLCNHCKTADDSPDIYLRHSIPNSQ
TIYKANSQGCNECNQGYSGRVGIYEVMPFTDKLKTSLINKPNALAIEDLARREGMRTLQESGIDKLLEGTTSYQELQRVL
YL

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=1054928 BCS84_RS04230 WP_016793486.1 853050..854738(-) (pilB) [Vibrio cyclitrophicus strain 10N.222.45.E8]
TTGGGACTCACCAACCTCCCAACCGTTCTACGTCAGGCTGGCTTACTTAGCCTGGCTCAAGAACAAGCCGTTGCGGAACA
TGTACAAGCTTCAGGTGTTTCGACACCTGAAGCTTTGCTCGTTCTAGATTTCTTCAGCGGCGATTCCTTAGCAAACAACA
TTAAAACCATCTTCGGTTTACCCTTAGTACAACTTGCCAATACGGACTATGAAACCTTATGTGACCAATTAGGGCTTCGC
GAACTGATTACTAAATATCGTGCTATCCCGATTTCAGTTTCTAGCTCCACTCTCACACTCGCTTCTGCCGACCCTACGGA
TTTACAAGCTGAAGATGACTTTCGATTCGCAACCGGATTACAGATTGAATTGGTTGTCGCTAATTACTCTGAATTAGAAG
GCGTAATACGCAAGCTGTATGGTCGATCTATTTCCGGGCAAGATTCTAAGCGTAAAGAGATCACCCAAGATGAGCTAGCT
AACCTCGTGAAGGTATCTGACGACGAAATAACGTCGATTGAAGATCTCAGCCAAGATGACTCTCCTGTGAGTCGCTTTAT
CAATCAGATTCTGGTTGATGCGGTGCGTAAAGGCGCATCGGACATCCACTTCGAACCTTACGAAGAGCATTACCGAGTTC
GCCTACGTTGTGATGGCATTCTTGTCGAGATCCAACAGCCCGCCTCTCATTTAAGCCGTCGTCTGTCAGCTCGCTTAAAG
ATCCTCGCTAAACTCGATATTGCCGAACGTCGCTTGCCTCAAGATGGTCGTATCAAACTGCGTTTAAATGACGAACTGGC
AATTGATATGCGTGTGTCTACGCTGCCAACATTGTGGGGAGAAAAGATCGTACTGCGATTGCTTGATAGCAGTGCGGCCA
ATCTCGATATCGATAAGCTCGGTTACAGCGAGGCTCAAAAAGCCCTCTACCTCAATGCACTAAAGCGCCCGCAAGGGATG
ATCTTAATGACCGGGCCAACCGGCAGCGGTAAAACGGTTTCTCTTTATACTGGACTTCGAGTACTCAACACCTCTGAACG
CAACATCTCAACAGCTGAAGACCCTGTAGAGATTAACCTGTGTGGCATCAATCAGGTACAAGTGACGCCTAAGATTGGCT
TTGGTTTCGCCGAGGCACTACGATCGTTTTTACGACAAGACCCAGACATCGTGATGGTTGGAGAAATCCGAGATTTAGAA
ACCGCCGAAATCGCGATCAAAGCCTCCCAAACCGGGCATTTAGTACTTTCAACACTGCACACTAACTCAGCCGCAGAAAC
CGTGACCCGGCTTGCTCATATGGGAATCGAGCCCTTCAACCTTGCTTCGTCTTTGAGCTTGATTATTGCTCAGCGTCTTG
CGAGACGCTTGTGTAACCACTGTAAAACCGCAGACGACTCACCAGACATTTATCTGCGCCACTCTATCCCAAACAGCCAA
ACCATCTACAAAGCCAACTCTCAAGGCTGTAATGAATGTAACCAAGGTTACTCAGGTCGAGTGGGTATCTATGAAGTGAT
GCCGTTTACCGACAAGCTAAAAACCAGCCTTATCAATAAACCCAATGCGCTAGCGATTGAAGATCTCGCACGTCGAGAAG
GCATGAGAACTTTACAAGAGTCAGGGATAGATAAACTGCTTGAAGGCACCACCAGCTACCAAGAGCTACAACGTGTTCTA
TACCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(189-456)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(12-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

73.357

100

0.735
  pilB Vibrio campbellii strain DS40M4

70.41

99.822

0.703
  pilB Vibrio parahaemolyticus RIMD 2210633

70.357

99.644

0.701
  pilB Acinetobacter baumannii D1279779

50.674

92.349

0.468
  pilB Legionella pneumophila strain ERS1305867

50.784

90.747

0.461
  pilB Acinetobacter baylyi ADP1

49.326

92.349

0.456
  pilF Neisseria gonorrhoeae MS11

44.286

99.644

0.441
  pilF Thermus thermophilus HB27

37.77

98.932

0.374

Multiple sequence alignment