Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BCV18_RS10450 Genome accession   NZ_CP170009
Coordinates   2249262..2250947 (-) Length   561 a.a.
NCBI ID   WP_065100288.1    Uniprot ID   -
Organism   Vibrio cyclitrophicus strain 10N.286.54.F2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2244262..2255947
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BCV18_RS10420 (BCV18_10420) rplS 2244773..2245126 (+) 354 WP_004735514.1 50S ribosomal protein L19 -
  BCV18_RS10425 (BCV18_10425) yacG 2245419..2245616 (-) 198 WP_010435916.1 DNA gyrase inhibitor YacG -
  BCV18_RS10430 (BCV18_10430) zapD 2245698..2246438 (-) 741 WP_010435917.1 cell division protein ZapD -
  BCV18_RS10435 (BCV18_10435) coaE 2246465..2247079 (-) 615 WP_010435920.1 dephospho-CoA kinase -
  BCV18_RS10440 (BCV18_10440) pilD 2247082..2247951 (-) 870 WP_016768203.1 prepilin peptidase Machinery gene
  BCV18_RS10445 (BCV18_10445) pilC 2248018..2249247 (-) 1230 WP_016768204.1 type II secretion system F family protein Machinery gene
  BCV18_RS10450 (BCV18_10450) pilB 2249262..2250947 (-) 1686 WP_065100288.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BCV18_RS10455 (BCV18_10455) pilA 2250947..2251420 (-) 474 WP_016790756.1 pilin Machinery gene
  BCV18_RS10460 (BCV18_10460) nadC 2251674..2252564 (-) 891 WP_004738163.1 carboxylating nicotinate-nucleotide diphosphorylase -
  BCV18_RS10465 (BCV18_10465) ampD 2252648..2253247 (+) 600 WP_016790757.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  BCV18_RS10470 (BCV18_10470) pdhR 2253729..2254490 (+) 762 WP_016768209.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62090.84 Da        Isoelectric Point: 5.4344

>NTDB_id=1054434 BCV18_RS10450 WP_065100288.1 2249262..2250947(-) (pilB) [Vibrio cyclitrophicus strain 10N.286.54.F2]
MLTNLPTVLRQAGLLSLTQEQAVAEHVEASGISTPEALLALNFFSSDSLANNIKTIFGLPLVRLANTDYESLCEQLGLRE
LITKYRAIPISVSSSTLTLASADPTDLQAEDDFRFATGLQVELVVANYSELEGAIRKLYGRSISGQYSKRKEITQDELAN
LVKVSDDEITSIEDLSQDDSPVSRFINQILVDAVRKGASDIHFEPYEENYRVRLRCDGILVEIQQPASHLSRRLSARLKI
LAKLDIAERRLPQDGRIKLRLNDELAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYSEAQKALYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRVLNTSERNISTAEDPVEINLCGINQVQVTPKIGFGFAEALRSFLRQDPDIVMVGEIRDLET
AEIAIKASQTGHLVLSTLHTNSAAETVTRLAHMGIEPFNLASSLSLIIAQRLARRLCNHCKTADDSPDIYLRHSIPNSQT
IYKANSQGCNECNQGYSGRVGIYEVMPFTDKLKTSLINKPNALAIEDLARREGMRTLQESGLDKLLEGTTSYQELQRVLY
L

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=1054434 BCV18_RS10450 WP_065100288.1 2249262..2250947(-) (pilB) [Vibrio cyclitrophicus strain 10N.286.54.F2]
ATGTTAACCAACCTCCCAACCGTTCTCCGTCAGGCTGGCTTACTTAGCCTGACTCAGGAACAAGCTGTTGCGGAACATGT
GGAAGCTTCAGGTATATCTACACCTGAAGCTTTACTCGCTCTGAATTTCTTCAGCAGCGATTCCTTAGCAAACAACATTA
AAACCATCTTCGGCTTACCGTTAGTACGATTAGCCAATACTGACTACGAATCCCTGTGTGAACAATTAGGACTTCGTGAA
CTGATTACTAAGTATCGTGCTATTCCAATTTCAGTTTCTAGCTCAACTCTCACACTCGCTTCTGCCGACCCTACGGATTT
ACAGGCTGAAGATGACTTCCGCTTTGCGACAGGATTGCAAGTTGAATTGGTTGTCGCTAACTACTCTGAATTAGAGGGTG
CAATACGAAAATTGTATGGTCGTTCTATTTCCGGGCAATACTCCAAACGCAAAGAGATCACCCAAGATGAGCTAGCTAAC
CTCGTCAAGGTATCTGACGACGAAATAACGTCGATAGAAGATCTCAGCCAAGATGACTCTCCTGTGAGTCGCTTTATCAA
TCAGATACTGGTTGATGCGGTGCGTAAAGGCGCATCGGACATCCACTTCGAACCTTATGAAGAAAACTACCGCGTACGTC
TACGTTGCGATGGCATCCTTGTCGAGATCCAACAACCCGCCTCTCATTTAAGCCGTCGTCTGTCAGCTCGCTTAAAGATC
CTCGCTAAACTCGATATTGCCGAACGTCGCTTACCTCAAGATGGCCGTATCAAACTGCGTTTAAATGACGAACTGGCAAT
TGATATGCGCGTATCAACGCTGCCTACCTTATGGGGCGAGAAGATCGTACTGCGATTGCTTGATAGCAGTGCGGCCAATC
TCGATATAGACAAGCTCGGTTACAGCGAGGCTCAAAAAGCCCTCTACCTCAATGCACTAAAGCGCCCGCAAGGGATGATC
TTAATGACCGGGCCAACCGGCAGCGGTAAAACGGTTTCTCTTTATACTGGACTTCGAGTACTCAACACCTCTGAACGCAA
CATCTCAACAGCTGAAGACCCAGTAGAGATTAACCTGTGTGGTATCAATCAGGTACAGGTGACGCCTAAGATTGGCTTTG
GTTTCGCCGAGGCACTACGATCGTTTTTACGACAAGACCCAGACATCGTGATGGTTGGAGAAATCCGAGATTTAGAAACC
GCCGAAATCGCGATCAAAGCCTCCCAAACCGGGCATTTAGTACTTTCAACACTGCACACTAACTCAGCCGCAGAAACCGT
GACCCGGCTTGCTCATATGGGAATCGAGCCCTTCAACCTTGCTTCGTCTTTGAGCTTGATTATTGCTCAGCGTCTTGCGA
GACGCTTGTGTAACCACTGTAAAACCGCAGACGACTCACCAGACATTTATCTGCGCCACTCTATCCCAAACAGCCAAACC
ATCTACAAAGCCAACTCTCAAGGCTGTAATGAATGTAACCAAGGTTACTCAGGTCGAGTCGGTATCTATGAAGTGATGCC
GTTTACCGACAAGCTAAAAACCAGCCTTATCAACAAACCCAATGCGCTAGCGATTGAAGATCTCGCGCGTAGAGAAGGCA
TGCGCACTCTGCAAGAGTCAGGGCTAGATAAACTGCTTGAAGGCACCACCAGCTATCAAGAGCTACAACGTGTTCTATAC
CTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

74.113

100

0.745
  pilB Vibrio campbellii strain DS40M4

71.174

100

0.713
  pilB Vibrio parahaemolyticus RIMD 2210633

70.996

100

0.711
  pilB Legionella pneumophila strain ERS1305867

48.007

98.396

0.472
  pilB Acinetobacter baumannii D1279779

50.867

92.513

0.471
  pilB Acinetobacter baylyi ADP1

49.715

93.939

0.467
  pilF Neisseria gonorrhoeae MS11

44.464

99.822

0.444
  pilF Thermus thermophilus HB27

38.964

92.87

0.362

Multiple sequence alignment