Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   BCV18_RS10455 Genome accession   NZ_CP170009
Coordinates   2250947..2251420 (-) Length   157 a.a.
NCBI ID   WP_016790756.1    Uniprot ID   -
Organism   Vibrio cyclitrophicus strain 10N.286.54.F2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2245947..2256420
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BCV18_RS10435 (BCV18_10435) coaE 2246465..2247079 (-) 615 WP_010435920.1 dephospho-CoA kinase -
  BCV18_RS10440 (BCV18_10440) pilD 2247082..2247951 (-) 870 WP_016768203.1 prepilin peptidase Machinery gene
  BCV18_RS10445 (BCV18_10445) pilC 2248018..2249247 (-) 1230 WP_016768204.1 type II secretion system F family protein Machinery gene
  BCV18_RS10450 (BCV18_10450) pilB 2249262..2250947 (-) 1686 WP_065100288.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BCV18_RS10455 (BCV18_10455) pilA 2250947..2251420 (-) 474 WP_016790756.1 pilin Machinery gene
  BCV18_RS10460 (BCV18_10460) nadC 2251674..2252564 (-) 891 WP_004738163.1 carboxylating nicotinate-nucleotide diphosphorylase -
  BCV18_RS10465 (BCV18_10465) ampD 2252648..2253247 (+) 600 WP_016790757.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  BCV18_RS10470 (BCV18_10470) pdhR 2253729..2254490 (+) 762 WP_016768209.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 157 a.a.        Molecular weight: 16745.10 Da        Isoelectric Point: 5.8402

>NTDB_id=1054435 BCV18_RS10455 WP_016790756.1 2250947..2251420(-) (pilA) [Vibrio cyclitrophicus strain 10N.286.54.F2]
MNNKSKRTNQKGFTLIELMIVVAIIGILSAFAVPAYQDYTKKATLSEFPKAAAAVKMAVELCAHESASDGDTFKTNCVST
TDNIPAAFDLNSMNILAIGGTSSGAVDVRVKATAIKGPIAANETYVMTATYTTNGITWESKCYIDAALNNQQETYCP

Nucleotide


Download         Length: 474 bp        

>NTDB_id=1054435 BCV18_RS10455 WP_016790756.1 2250947..2251420(-) (pilA) [Vibrio cyclitrophicus strain 10N.286.54.F2]
ATGAATAACAAAAGTAAAAGAACAAATCAGAAAGGCTTTACGCTGATTGAATTGATGATTGTAGTGGCGATTATTGGGAT
CTTATCGGCGTTTGCTGTTCCTGCTTATCAAGATTACACGAAAAAAGCGACTCTATCCGAATTTCCGAAAGCAGCCGCTG
CTGTAAAAATGGCTGTTGAATTATGTGCACATGAAAGTGCTTCGGATGGTGATACATTTAAAACAAATTGTGTAAGTACT
ACCGACAATATTCCCGCAGCATTTGACCTTAACAGTATGAACATATTAGCTATTGGAGGCACCAGTTCAGGAGCTGTAGA
TGTAAGAGTCAAAGCGACTGCAATCAAAGGGCCTATTGCTGCAAATGAAACTTACGTAATGACAGCAACGTATACTACAA
ATGGCATCACGTGGGAGAGTAAGTGTTATATAGACGCAGCATTGAACAATCAACAAGAGACCTACTGCCCATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(8-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio campbellii strain DS40M4

58.17

97.452

0.567
  pilA Haemophilus influenzae 86-028NP

39.333

95.541

0.376
  comP Acinetobacter baylyi ADP1

40.426

89.809

0.363

Multiple sequence alignment