Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BCV31_RS06285 Genome accession   NZ_CP169999
Coordinates   1485139..1486824 (+) Length   561 a.a.
NCBI ID   WP_016795169.1    Uniprot ID   -
Organism   Vibrio cyclitrophicus strain 10N.286.55.C10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1480139..1491824
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BCV31_RS06265 (BCV31_06265) pdhR 1481655..1482416 (-) 762 WP_016768209.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  BCV31_RS06270 (BCV31_06270) ampD 1482898..1483497 (-) 600 WP_102409372.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  BCV31_RS06275 (BCV31_06275) nadC 1483581..1484471 (+) 891 WP_004738163.1 carboxylating nicotinate-nucleotide diphosphorylase -
  BCV31_RS06280 (BCV31_06280) pilA 1484725..1485135 (+) 411 WP_016783772.1 pilin Machinery gene
  BCV31_RS06285 (BCV31_06285) pilB 1485139..1486824 (+) 1686 WP_016795169.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BCV31_RS06290 (BCV31_06290) pilC 1486839..1488068 (+) 1230 WP_016795168.1 type II secretion system F family protein Machinery gene
  BCV31_RS06295 (BCV31_06295) pilD 1488135..1489004 (+) 870 WP_016795167.1 prepilin peptidase Machinery gene
  BCV31_RS06300 (BCV31_06300) coaE 1489007..1489621 (+) 615 WP_016795166.1 dephospho-CoA kinase -
  BCV31_RS06305 (BCV31_06305) zapD 1489648..1490388 (+) 741 WP_010435917.1 cell division protein ZapD -
  BCV31_RS06310 (BCV31_06310) yacG 1490470..1490667 (+) 198 WP_010435916.1 DNA gyrase inhibitor YacG -
  BCV31_RS06315 (BCV31_06315) rplS 1490948..1491301 (-) 354 WP_004735514.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62128.88 Da        Isoelectric Point: 5.3078

>NTDB_id=1054231 BCV31_RS06285 WP_016795169.1 1485139..1486824(+) (pilB) [Vibrio cyclitrophicus strain 10N.286.55.C10]
MLTNLPTVLRQAGLLSLTQEQAVAEQVQASGISTPEALLALDFFSGDSLANNIKTIFGLPLVQLANTDYETLRDQLGLRE
LITKYRAIPISVSSSTLTLASADPTDLQAEDDFRFATGLQIELVVANYSELEGAIRKLYGRSISGHDSKRKEITQDELAN
LVKVSDDEITSIEDLSQDDSPVSRFINQILVDAVRKGASDIHFEPYEEHYRVRLRCDGILVEIQQPASHLSRRLSARLKI
LAKLDIAERRLPQDGRIKLRLNDELAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYSEAQKALYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRVLNTSERNISTAEDPVEINLCGINQVQVTPKIGFGFAEALRSFLRQDPDIVMVGEIRDLET
AEIAIKASQTGHLVLSTLHTNSAAETVTRLAHMGIEPFNLASSLSLIIAQRLARRLCNHCKETDDSPDIFLRHSIPNSQI
IYKANSQGCNECNQGYSGRVGIYEVMPFTDKLKTSLINKPNALAIEDLARREGMRTLQESGIDKLLEGTTSYQELQRVLY
L

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=1054231 BCV31_RS06285 WP_016795169.1 1485139..1486824(+) (pilB) [Vibrio cyclitrophicus strain 10N.286.55.C10]
ATGCTCACTAACCTCCCAACCGTTCTACGTCAGGCTGGCTTACTTAGCCTGACTCAAGAACAAGCCGTTGCGGAACAAGT
ACAAGCTTCAGGCATTTCGACACCTGAAGCTTTGCTCGCTCTGGATTTCTTCAGCGGCGATTCCTTAGCAAACAACATTA
AAACCATCTTCGGCTTACCCTTAGTACAACTTGCCAATACGGACTATGAAACCTTACGTGACCAATTAGGGCTTCGCGAA
CTGATTACTAAATATCGTGCTATCCCGATTTCAGTTTCTAGCTCCACCCTCACACTCGCTTCTGCCGACCCTACGGATTT
ACAAGCTGAAGATGACTTCCGCTTTGCGACAGGATTACAGATTGAATTGGTTGTTGCTAATTACTCTGAATTAGAAGGTG
CGATACGCAAGCTGTATGGCCGCTCTATTTCAGGGCATGATTCTAAGCGTAAAGAGATCACCCAAGATGAGTTAGCTAAC
CTCGTGAAGGTATCTGACGACGAAATAACGTCGATTGAAGATCTCAGCCAAGATGACTCTCCTGTGAGTCGCTTTATCAA
TCAGATTCTGGTTGATGCGGTGCGTAAAGGCGCATCGGACATCCACTTCGAACCTTACGAAGAGCATTACCGTGTGCGAC
TACGTTGTGACGGCATATTGGTTGAGATACAGCAGCCTGCTTCCCATTTAAGCCGTCGTCTGTCCGCTCGTCTGAAAATA
CTCGCCAAGCTTGATATTGCCGAACGTCGCTTACCTCAAGATGGTCGTATTAAACTGCGTTTAAATGACGAACTAGCAAT
TGATATGCGCGTATCAACGCTGCCTACCTTATGGGGCGAGAAGATCGTACTGCGATTGCTTGATAGCAGTGCGGCCAATC
TCGATATCGATAAGCTCGGTTACAGCGAGGCTCAAAAAGCCCTCTACCTCAATGCACTAAAGCGCCCGCAAGGGATGATC
TTAATGACCGGGCCAACCGGCAGCGGTAAAACGGTTTCTCTTTATACTGGACTTCGAGTACTCAACACCTCTGAACGCAA
CATCTCAACAGCTGAAGACCCAGTAGAGATTAACCTGTGTGGTATCAATCAGGTACAGGTGACGCCTAAGATTGGCTTTG
GTTTCGCCGAGGCACTACGATCGTTTTTACGACAAGACCCAGACATCGTGATGGTTGGAGAAATCCGAGATTTAGAAACC
GCCGAAATCGCGATCAAAGCCTCCCAAACCGGGCATTTAGTACTTTCAACACTGCACACTAACTCAGCCGCAGAAACCGT
GACTCGGCTTGCTCATATGGGAATAGAGCCCTTCAACCTTGCTTCGTCATTGAGCCTAATCATAGCCCAACGACTGGCAA
GACGCTTGTGCAACCATTGCAAAGAAACAGACGACTCGCCGGACATATTTCTGCGTCACTCGATTCCTAACAGCCAAATA
ATCTACAAAGCTAACTCTCAAGGGTGTAATGAATGTAACCAAGGTTACTCAGGTCGAGTGGGTATCTATGAAGTGATGCC
GTTTACCGACAAGCTAAAAACCAGCCTTATCAATAAACCCAATGCGCTAGCGATTGAAGATCTCGCACGCCGAGAAGGCA
TGAGAACTTTACAAGAGTCAGGGATAGATAAACTGCTTGAAGGCACCACCAGCTACCAAGAGCTACAACGTGTTCTATAC
CTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(12-141)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

73.759

100

0.742
  pilB Vibrio campbellii strain DS40M4

70.819

100

0.709
  pilB Vibrio parahaemolyticus RIMD 2210633

70.641

100

0.708
  pilB Legionella pneumophila strain ERS1305867

48.282

98.574

0.476
  pilB Acinetobacter baumannii D1279779

50.482

92.513

0.467
  pilB Acinetobacter baylyi ADP1

50

93.405

0.467
  pilF Neisseria gonorrhoeae MS11

43.816

100

0.442
  pilF Thermus thermophilus HB27

39.347

92.87

0.365

Multiple sequence alignment