Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   ACEY2B_RS05925 Genome accession   NZ_CP169315
Coordinates   1209381..1210229 (-) Length   282 a.a.
NCBI ID   WP_015426410.1    Uniprot ID   -
Organism   Lactococcus lactis strain IFLLLBEK1     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1204381..1215229
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEY2B_RS05910 (ACEY2B_05910) xerS 1204457..1205527 (+) 1071 WP_003131037.1 tyrosine recombinase XerS -
  ACEY2B_RS05915 (ACEY2B_05915) trmFO 1205572..1206915 (-) 1344 WP_003131150.1 methylenetetrahydrofolate--tRNA-(uracil(54)- C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO -
  ACEY2B_RS05920 (ACEY2B_05920) topA 1206987..1209119 (-) 2133 WP_015426411.1 type I DNA topoisomerase -
  ACEY2B_RS05925 (ACEY2B_05925) dprA 1209381..1210229 (-) 849 WP_015426410.1 DNA-processing protein DprA Machinery gene
  ACEY2B_RS05930 (ACEY2B_05930) - 1210375..1210755 (-) 381 WP_015426409.1 RidA family protein -
  ACEY2B_RS05935 (ACEY2B_05935) budA 1210796..1211506 (-) 711 WP_015426408.1 acetolactate decarboxylase -
  ACEY2B_RS05940 (ACEY2B_05940) ilvA 1211622..1212872 (-) 1251 WP_015426407.1 threonine ammonia-lyase IlvA -
  ACEY2B_RS05945 (ACEY2B_05945) ilvC 1212928..1213950 (-) 1023 WP_015426406.1 ketol-acid reductoisomerase -
  ACEY2B_RS05950 (ACEY2B_05950) ilvN 1213996..1214472 (-) 477 WP_015426405.1 acetolactate synthase small subunit -

Sequence

Protein


Download         Length: 282 a.a.        Molecular weight: 32159.28 Da        Isoelectric Point: 9.5086

>NTDB_id=1050482 ACEY2B_RS05925 WP_015426410.1 1209381..1210229(-) (dprA) [Lactococcus lactis strain IFLLLBEK1]
MITNFDLYRWKKAGMTNLGVNKLLKFFHRYDKKISLRQMGQVAQLKSIPNFIEQYKNQDIIKLREEYKIYPSFSLLDEIY
PERLKEIYNPPVLLFYQGDIRLLKTPKLAFVGSRQASSQGIKAVQKIVTELNQNFTIVSGLAKGIDTASHLSAIKNKIPT
IAVIGTGLDIFYPLENRKIQEYLAKNQLILSEYSVGEKPLRYHFPERNRIIAGLSHGVVVIEAKLRSGSLITCERALEEG
RDIFAVPGNIADGFSDGCNHLIQQGAKLIYQAQDILEEYLYD

Nucleotide


Download         Length: 849 bp        

>NTDB_id=1050482 ACEY2B_RS05925 WP_015426410.1 1209381..1210229(-) (dprA) [Lactococcus lactis strain IFLLLBEK1]
ATGATAACAAATTTTGATTTATATCGTTGGAAAAAAGCTGGCATGACCAATTTAGGAGTAAATAAACTTTTAAAATTTTT
TCATAGATATGATAAAAAAATAAGTTTAAGACAAATGGGACAAGTAGCACAACTTAAATCAATTCCCAACTTTATTGAAC
AATATAAAAATCAAGACATAATTAAACTGAGAGAAGAGTATAAAATATACCCATCATTTTCTCTTCTTGATGAAATTTAT
CCCGAAAGGCTCAAGGAAATTTACAATCCACCTGTTCTATTATTTTATCAAGGAGATATAAGACTCTTAAAAACTCCTAA
ATTAGCTTTTGTTGGTAGTCGACAAGCAAGCTCACAAGGAATTAAAGCCGTTCAAAAAATTGTGACAGAGTTGAACCAAA
ATTTTACGATTGTCAGCGGACTCGCTAAAGGAATTGATACAGCAAGTCATCTTTCTGCCATTAAAAATAAAATCCCTACA
ATTGCAGTTATCGGTACAGGCTTAGATATCTTTTATCCTTTAGAAAATCGAAAAATTCAAGAATATCTTGCTAAAAACCA
ACTAATTCTATCAGAATATTCAGTCGGAGAAAAACCACTTCGCTATCATTTTCCTGAACGCAATCGTATCATTGCGGGAC
TTTCACATGGTGTAGTAGTGATAGAAGCAAAGTTACGAAGTGGTAGTTTAATTACTTGTGAGCGCGCTCTGGAAGAAGGA
AGAGATATTTTCGCTGTACCAGGAAACATAGCAGATGGATTTTCAGATGGCTGTAATCATCTAATTCAACAAGGAGCAAA
ATTAATCTACCAAGCCCAAGATATCTTAGAAGAATATTTGTATGATTAA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(76-270)

DNA processing protein A sterile alpha motif domain (SAM_DprA)

(2-63)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Lactococcus lactis subsp. cremoris KW2

86.525

100

0.865
  dprA Streptococcus mutans UA159

60.072

98.582

0.592
  dprA/cilB/dalA Streptococcus mitis SK321

54.643

99.291

0.543
  dprA/cilB/dalA Streptococcus pneumoniae R6

53.929

99.291

0.535
  dprA/cilB/dalA Streptococcus mitis NCTC 12261

53.929

99.291

0.535
  dprA/cilB/dalA Streptococcus pneumoniae TIGR4

53.929

99.291

0.535
  dprA/cilB/dalA Streptococcus pneumoniae Rx1

53.929

99.291

0.535
  dprA/cilB/dalA Streptococcus pneumoniae D39

53.929

99.291

0.535
  dprA Legionella pneumophila strain ERS1305867

38.768

97.872

0.379
  dprA Haemophilus influenzae Rd KW20

38.519

95.745

0.369
  dprA Bacillus subtilis subsp. subtilis str. 168

44.206

82.624

0.365
  dprA Latilactobacillus sakei subsp. sakei 23K

40

90.426

0.362

Multiple sequence alignment