Detailed information NTG1050206

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ACETK7_RS03280
• Genome accession: NZ_CP169287
• Coordinates: 726629..727738 (-)
• Length: 369 a.a.
• NCBI ID: WP_214384828.1
• Uniprot ID: -
• Organism: Pseudomonas hormoni strain G20-18_CNT1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 721629..732738

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACETK7_RS03265 (ACETK7_03265)	-	722198..723589 (+)	1392	WP_214382982.1	GntP family permease	-
ACETK7_RS03270 (ACETK7_03270)	hbdH	723597..724370 (+)	774	WP_214382980.1	3-hydroxybutyrate dehydrogenase	-
ACETK7_RS03275 (ACETK7_03275)	-	724547..726502 (+)	1956	WP_214382978.1	acetoacetate--CoA ligase	-
ACETK7_RS03280 (ACETK7_03280)	pilU	726629..727738 (-)	1110	WP_214384828.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ACETK7_RS03285 (ACETK7_03285)	-	727859..730396 (+)	2538	WP_214382976.1	PAS domain-containing protein	-
ACETK7_RS03290 (ACETK7_03290)	-	730462..730737 (+)	276	WP_007898282.1	peptidylprolyl isomerase	-
ACETK7_RS03295 (ACETK7_03295)	-	730901..731836 (-)	936	WP_214382974.1	sugar kinase	-

Sequence

Protein

• Length: 369 a.a.
• Molecular weight: 41135.04 Da
• Isoelectric Point: 5.9095

>NTDB_id=1050206 ACETK7_RS03280 WP_214384828.1 726629..727738(-) (pilU) [Pseudomonas hormoni strain G20-18_CNT1]
MEIDGLLKILASQEGSDLYLSTGAPPCARFDGVLKPLSDQPFKPGEITAIAMTIMDAEQRLEFDRELEMNLAISLTGVGR
FRVNIFKQRNDVSIVMRNVKLDIPRFEDLQLPAVLLETVMLKQGLMLFVGATDSGKSTSLAALIDYRNRHSSGHIITIED
PVEYIHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFADTGHLVISTLHAHNAHQALDRI
INFFPEERRAQLLHDLGNNLKAFVSQRLVRTRDGQRRAAVEVMLGSPTIGDLIRRNEFDELKGIMEKSAEQGMQTFDGAL
FALVEEGAIDEEEALKHADSVNNLRLRLKLHADASPGPNAAPGEWGLMD

Nucleotide

• Length: 1110 bp

>NTDB_id=1050206 ACETK7_RS03280 WP_214384828.1 726629..727738(-) (pilU) [Pseudomonas hormoni strain G20-18_CNT1]
ATGGAAATCGATGGGCTTTTAAAGATTCTGGCCAGCCAGGAAGGTTCCGATCTGTACCTGTCCACCGGCGCGCCGCCCTG
CGCACGGTTTGATGGTGTGCTCAAACCCTTGTCCGATCAGCCGTTCAAGCCGGGGGAAATCACGGCGATTGCCATGACCA
TCATGGACGCCGAGCAACGGCTGGAGTTCGATCGTGAACTGGAGATGAACCTGGCGATCTCTTTGACGGGTGTCGGGCGT
TTTCGGGTCAACATTTTCAAACAACGCAACGACGTGTCGATCGTGATGCGTAATGTCAAACTCGACATTCCGCGCTTCGA
AGACCTCCAGTTGCCCGCGGTGCTGCTGGAAACGGTCATGCTCAAACAAGGGCTGATGCTGTTCGTCGGCGCCACCGATT
CGGGAAAGTCTACCTCACTGGCGGCGTTGATCGACTACCGAAACCGCCACAGCAGCGGCCACATCATCACCATCGAAGAC
CCGGTCGAATATATTCATCGGCACAAGAAATCGATCATCAATCAGCGCGAGGTTGGCGTGGATACCCGCAGTTTCCATGC
TGCGTTGAAGAACACCCTGCGCCAGGCGCCGGATGTGGTGCTGATCGGCGAGATCCGCGACCGCGAAACCATGGAGCATG
CGCTGGCGTTTGCCGATACCGGGCACCTGGTGATTTCTACCTTGCATGCCCACAACGCCCATCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGGAGGAACGACGGGCGCAACTGCTGCATGACCTGGGCAACAATCTCAAAGCGTTTGTTTCCCAACG
GCTTGTGCGCACCCGTGATGGGCAACGGAGGGCGGCGGTGGAAGTGATGCTCGGTTCGCCGACCATTGGTGACTTGATCC
GGCGCAACGAATTCGACGAGCTCAAAGGAATCATGGAGAAGTCCGCCGAGCAGGGCATGCAGACGTTCGATGGCGCGTTG
TTTGCGTTGGTTGAAGAGGGCGCGATCGATGAGGAGGAAGCGCTGAAGCACGCGGACTCGGTGAACAATTTGCGCCTGCG
GCTGAAGCTGCATGCCGACGCATCACCAGGACCCAATGCAGCACCCGGCGAATGGGGACTGATGGACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (79-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	54.648	96.206	0.526
pilU	Acinetobacter baylyi ADP1	51.261	96.748	0.496
pilU	Vibrio cholerae strain A1552	50.571	94.851	0.48
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	38.122	98.103	0.374
pilT	Pseudomonas aeruginosa PAK	39.826	93.225	0.371
pilT	Vibrio cholerae strain A1552	40.841	90.244	0.369
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	40.841	90.244	0.369
pilT	Pseudomonas stutzeri DSM 10701	39.244	93.225	0.366