Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ACEUIV_RS19230 Genome accession   NZ_CP169221
Coordinates   4181750..4183900 (-) Length   716 a.a.
NCBI ID   WP_022995173.1    Uniprot ID   A0A9Q3W083
Organism   Alloalcanivorax xenomutans strain A28     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4176750..4188900
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEUIV_RS19215 - 4178745..4180097 (-) 1353 WP_035450449.1 SPOR domain-containing protein -
  ACEUIV_RS19220 aroB 4180084..4181166 (-) 1083 WP_026948239.1 3-dehydroquinate synthase -
  ACEUIV_RS19225 aroK 4181217..4181753 (-) 537 WP_026948240.1 shikimate kinase AroK -
  ACEUIV_RS19230 pilQ 4181750..4183900 (-) 2151 WP_022995173.1 type IV pilus secretin PilQ Machinery gene
  ACEUIV_RS19235 - 4183921..4184454 (-) 534 WP_022995172.1 pilus assembly protein PilP -
  ACEUIV_RS19240 - 4184472..4185158 (-) 687 WP_022995171.1 type 4a pilus biogenesis protein PilO -
  ACEUIV_RS19245 - 4185145..4185729 (-) 585 WP_022995170.1 PilN domain-containing protein -
  ACEUIV_RS19250 pilM 4185726..4186793 (-) 1068 WP_026948241.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 716 a.a.        Molecular weight: 78045.40 Da        Isoelectric Point: 4.5897

>NTDB_id=1049885 ACEUIV_RS19230 WP_022995173.1 4181750..4183900(-) (pilQ) [Alloalcanivorax xenomutans strain A28]
MKICNAIKNRFAPRRWGIRVLALLACCTASLASAATITALDANMLPGDGMEVRVRFDGAAPEVKGYSIERPARIALDLVG
ADSALKTRQHNLGTGNARSVTVVEAKNRTRLIFNLDQLTGYSTEVEGQELVVVLGRDRAPAGDPARADQEAAAAPPRGVQ
ESIRNIDFRRGSDGEGRVVVTLSKPSIPVDVKEEGGRIQARFVGAGVGNELVRRLDVTDFATPVQYVETRSDDGSTVITI
EPTGTWEYLAYQADNEFSINIKPLTRGEAERKKRESFKYTGEKLSLNFQDIEVRSVLQLIADFTELNLVASDTVTGKITL
RLQSVPWDQALDLILKTKGLDKRKVGNVLLVAPADEIAAREQLELESQKKIASLAPLRTEFISINYARAEELKTLITGES
GATALISERGSVSVDQRTNTLIIQDTVAKLQEIRDLIQQLDIPVQQVLIEARVVVATTDLSDEFGVRWGGLGYDGNSLAN
DGRSMAISGRLTSLRELHYANVDRDDDGSLGEMEEFDEDTNNDMIVDMGVDSADATRFAIGFTSLSSGLIELELSALESE
GRGELVATPKVLTADQQPAVIASGQQIPFNVATSSGATAVEFIEAELRLEVTPQVTPEGDVIMQLKVNNDALGSDLDNPT
ISTNRVETTVLVNDGETVVLGGIFQQEQINSLIKTPFLGDIPWLGALFRKKVKRDSKQELLIFITPRLVKETMARR

Nucleotide


Download         Length: 2151 bp        

>NTDB_id=1049885 ACEUIV_RS19230 WP_022995173.1 4181750..4183900(-) (pilQ) [Alloalcanivorax xenomutans strain A28]
ATGAAGATCTGCAATGCAATAAAAAACCGATTCGCTCCCCGGCGCTGGGGAATCCGGGTGCTGGCGCTGCTGGCTTGCTG
CACCGCCTCGCTGGCGTCGGCGGCCACCATTACCGCCCTCGACGCCAACATGCTGCCGGGGGACGGCATGGAAGTGAGAG
TGCGCTTTGATGGCGCCGCGCCGGAGGTGAAGGGCTACAGCATCGAACGCCCGGCCCGCATCGCTCTCGACCTGGTGGGC
GCGGACAGCGCCCTGAAAACCCGCCAACACAATCTGGGCACCGGCAATGCCCGCAGTGTCACCGTGGTGGAGGCGAAGAA
TCGCACCCGGCTGATCTTCAATCTGGATCAACTGACCGGTTACAGCACCGAAGTGGAAGGGCAGGAGCTGGTGGTGGTGC
TCGGCCGCGATCGTGCGCCGGCCGGTGATCCGGCCAGGGCGGATCAGGAAGCCGCCGCCGCGCCGCCGCGGGGCGTGCAG
GAGAGCATTCGCAACATCGATTTTCGCCGCGGCAGTGACGGCGAAGGCCGTGTCGTGGTGACACTGTCGAAACCGTCCAT
CCCGGTGGACGTGAAGGAAGAAGGCGGCCGTATCCAGGCGCGCTTCGTGGGCGCCGGGGTTGGCAATGAACTGGTGCGCC
GTCTCGACGTCACCGATTTCGCCACGCCGGTACAGTATGTGGAAACCCGCAGCGATGACGGCAGTACGGTGATCACCATC
GAACCCACTGGCACCTGGGAATATCTGGCCTATCAGGCGGACAACGAGTTCAGCATCAATATCAAGCCGCTGACCCGCGG
CGAAGCGGAACGCAAGAAGCGTGAGTCCTTCAAGTACACCGGTGAGAAGCTGTCACTGAACTTCCAGGACATCGAGGTGC
GTTCGGTGCTGCAGTTGATCGCCGATTTCACTGAGCTGAACCTGGTGGCTTCCGATACGGTGACGGGCAAGATTACTTTG
CGGCTGCAGAGCGTGCCCTGGGATCAGGCTCTGGACCTGATTCTCAAGACCAAGGGGCTGGATAAACGCAAGGTGGGCAA
TGTGCTGCTGGTGGCGCCGGCGGACGAGATCGCCGCGCGCGAACAGCTGGAGCTGGAAAGCCAGAAGAAGATCGCCTCAC
TGGCGCCGCTGCGCACGGAGTTCATCAGCATCAACTACGCTCGCGCGGAGGAACTGAAGACACTGATTACCGGGGAAAGC
GGCGCCACCGCGCTGATCAGCGAGCGCGGTTCGGTATCCGTGGACCAGCGCACCAATACCCTGATCATCCAGGACACCGT
GGCCAAGCTGCAGGAGATTCGTGATCTGATCCAGCAACTGGACATTCCGGTCCAGCAGGTGCTGATCGAGGCGCGGGTGG
TGGTGGCCACCACCGATCTGTCCGATGAGTTCGGGGTTCGTTGGGGCGGTCTCGGTTACGACGGCAATTCTCTCGCCAAT
GATGGCCGTTCCATGGCGATTTCCGGCCGCCTCACCTCCTTGCGGGAGCTGCATTACGCCAACGTTGACCGTGATGACGA
CGGCTCCCTGGGGGAAATGGAGGAATTCGACGAGGACACCAATAACGACATGATCGTCGACATGGGAGTGGATTCCGCCG
ATGCCACCCGTTTCGCCATTGGTTTCACCTCACTGAGCTCCGGACTGATCGAGCTGGAGCTGTCGGCGCTGGAATCCGAG
GGGAGGGGGGAGCTGGTGGCCACGCCCAAGGTGCTCACCGCAGATCAGCAGCCGGCGGTGATCGCCTCCGGTCAGCAGAT
CCCGTTCAACGTGGCCACCTCCTCCGGCGCCACCGCGGTGGAGTTCATCGAGGCGGAGTTGCGCTTGGAAGTGACGCCGC
AGGTGACCCCGGAAGGGGACGTGATCATGCAGCTCAAGGTCAATAACGATGCCCTGGGCTCGGATCTGGACAACCCCACC
ATCAGCACCAACCGGGTTGAGACCACGGTGTTGGTGAATGACGGCGAAACCGTGGTTTTGGGCGGCATTTTCCAGCAGGA
ACAGATAAACTCCCTGATCAAAACCCCCTTCCTGGGTGATATTCCCTGGTTGGGAGCCCTGTTCCGCAAGAAAGTGAAGC
GTGACAGCAAGCAGGAATTGCTGATATTCATCACGCCACGCTTGGTAAAAGAGACAATGGCCCGTCGGTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(307-353)

AMIN domain (AMIN)

(48-133)

Bacterial type II/III secretion system short domain (Secretin_N)

(380-446)

Bacterial type II and III secretion system protein (Secretin)

(556-710)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

49.02

99.721

0.489
  comQ Acinetobacter baylyi ADP1

41.828

100

0.422
  pilQ Acinetobacter baumannii D1279779

40.841

100

0.42
  pilQ Acinetobacter baumannii strain A118

40.841

100

0.42
  pilQ Legionella pneumophila strain Lp02

37.662

96.788

0.365
  pilQ Legionella pneumophila strain ERS1305867

38.012

95.531

0.363

Multiple sequence alignment