Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACEUIV_RS16720 Genome accession   NZ_CP169221
Coordinates   3657268..3658980 (+) Length   570 a.a.
NCBI ID   WP_022995252.1    Uniprot ID   -
Organism   Alloalcanivorax xenomutans strain A28     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3652268..3663980
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEUIV_RS16705 - 3652544..3654754 (+) 2211 WP_026948631.1 TRAP transporter permease -
  ACEUIV_RS16710 - 3654989..3656344 (-) 1356 WP_155854803.1 O-antigen ligase -
  ACEUIV_RS16715 - 3656469..3656906 (-) 438 WP_026948629.1 pilin -
  ACEUIV_RS16720 pilB 3657268..3658980 (+) 1713 WP_022995252.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACEUIV_RS16725 pilC 3659051..3660274 (+) 1224 WP_022995251.1 type II secretion system F family protein Machinery gene
  ACEUIV_RS16730 pilD 3660302..3661165 (+) 864 WP_022995250.1 A24 family peptidase Machinery gene
  ACEUIV_RS16735 coaE 3661379..3661984 (+) 606 WP_022995249.1 dephospho-CoA kinase -
  ACEUIV_RS16740 - 3661974..3662168 (+) 195 WP_022995248.1 DNA gyrase inhibitor YacG -
  ACEUIV_RS16745 - 3662169..3663119 (-) 951 WP_026948627.1 Nudix family hydrolase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 62313.20 Da        Isoelectric Point: 5.6259

>NTDB_id=1049879 ACEUIV_RS16720 WP_022995252.1 3657268..3658980(+) (pilB) [Alloalcanivorax xenomutans strain A28]
MSSPQSSLSGLARRLVNEGHLSTEQVMEAAGAARREQMPLVRRLVGSGLLTAATVARASAEEFGDPVIDLDAFSPDVLVK
DLVDTKLIARHTVLPLYKRGSRLFVAVSDPTNLPALEEIRFNTGLNVETVIVEDDKLTRWIDRLESEAEGKAFADLDEGL
DNLDVANGEPAADAKDEANGADDAPIVRFVNKLLLDAIKGGASDLHFEPYEKTYRVRFRQDGVLQTMSKPPINSASKIAA
RLKVMARLDISERRVPQDGRIKLKISKNRAIDFRVNTCPTLFGEKIVLRILDPDSAKMGIDALGYEPEQKDMYMKALNQP
QGMILVTGPTGSGKTVSLYTGVNILNTPERNISTAEDPVEINLEGINQVNVNPKQGLDFSSALRAFLRQDPDVILVGEIR
DLETAEIAVKAAQTGHLVLSTLHTNSAPETLTRLRNMGVPSFNIATSVSLIIAQRLARRLCSHCKEAVDVPKPSLLAMGF
TEQDIESGFTVFGPKGCQKCNNGYKGRVGIYEVVRVTDGIARIIMQDGNSIEIADQCRKEGFNDLRRSGLLKVMQGVTSL
EEMDRVTSGH

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=1049879 ACEUIV_RS16720 WP_022995252.1 3657268..3658980(+) (pilB) [Alloalcanivorax xenomutans strain A28]
ATGAGCAGTCCTCAATCATCCCTGAGCGGCCTCGCCCGCCGCCTGGTCAACGAAGGCCATCTGTCCACCGAGCAGGTGAT
GGAAGCGGCCGGCGCCGCCCGCCGGGAGCAGATGCCGCTGGTCCGGCGTCTGGTGGGCTCGGGTCTGCTGACCGCGGCCA
CCGTGGCACGGGCTTCCGCCGAGGAGTTCGGGGATCCGGTGATAGATCTGGATGCGTTTTCCCCGGATGTCCTGGTCAAG
GACCTGGTGGATACCAAGCTGATCGCCCGCCACACCGTCCTGCCCCTGTACAAAAGGGGCAGCCGTTTGTTCGTCGCCGT
CTCCGACCCCACCAATCTGCCGGCGCTGGAGGAAATCCGCTTCAATACCGGCCTCAACGTGGAAACAGTGATTGTCGAGG
ACGACAAGCTGACCCGTTGGATCGACCGGCTGGAGTCGGAGGCGGAGGGCAAGGCGTTCGCCGATCTCGACGAGGGACTG
GACAATCTGGACGTGGCCAACGGTGAACCGGCCGCGGACGCCAAGGACGAGGCCAACGGCGCCGACGATGCCCCCATCGT
TCGCTTCGTCAACAAGCTGCTGCTGGACGCCATCAAGGGCGGCGCCTCGGACCTGCACTTCGAACCCTACGAAAAGACCT
ACCGGGTACGGTTCCGTCAGGACGGAGTGTTGCAGACCATGTCCAAGCCGCCGATCAACAGCGCCAGCAAGATCGCCGCC
CGGCTCAAGGTGATGGCGCGCCTGGATATTTCCGAACGGCGGGTTCCCCAGGACGGTCGCATCAAGCTGAAGATTTCCAA
GAATCGCGCCATCGATTTCCGGGTCAACACCTGCCCCACTCTGTTTGGCGAAAAGATCGTGCTGCGGATTCTGGATCCGG
ACAGCGCCAAGATGGGTATTGATGCGCTGGGCTATGAACCCGAGCAGAAAGACATGTACATGAAGGCCCTGAACCAGCCC
CAGGGCATGATTCTGGTCACCGGCCCCACCGGTTCCGGTAAGACCGTGTCTCTTTATACCGGCGTGAACATTCTCAACAC
GCCGGAGCGCAACATCTCCACCGCCGAGGATCCGGTGGAAATCAACCTGGAAGGCATCAATCAGGTCAACGTGAATCCCA
AGCAGGGGCTGGATTTCTCGTCCGCGCTGCGGGCGTTTCTGCGTCAGGATCCCGACGTCATTCTGGTGGGGGAGATCCGC
GATCTGGAGACCGCGGAAATCGCCGTGAAAGCGGCCCAGACCGGCCACCTGGTGTTATCGACCCTGCACACCAACAGCGC
TCCGGAAACCCTGACCCGTCTGCGTAACATGGGGGTGCCGTCCTTCAATATCGCCACTTCGGTGAGTCTGATCATCGCCC
AGCGCCTGGCCCGCCGCCTTTGCAGCCACTGCAAGGAAGCGGTGGATGTGCCCAAACCCTCGCTGCTTGCGATGGGTTTC
ACGGAACAGGACATCGAGAGTGGTTTTACTGTGTTCGGCCCGAAAGGCTGCCAAAAATGCAATAACGGCTACAAGGGCCG
GGTGGGGATCTATGAGGTGGTCCGGGTCACGGACGGTATTGCCCGCATCATCATGCAGGATGGCAATTCCATCGAGATCG
CCGACCAGTGCCGCAAGGAAGGCTTCAACGATTTGCGCCGCTCCGGGCTACTCAAGGTGATGCAGGGGGTCACCAGTCTG
GAGGAAATGGACCGGGTCACCAGCGGCCACTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-149)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

62.917

99.825

0.628
  pilB Acinetobacter baylyi ADP1

61.456

98.772

0.607
  pilB Legionella pneumophila strain ERS1305867

52.705

100

0.53
  pilB Vibrio cholerae strain A1552

54.599

89.649

0.489
  pilF Neisseria gonorrhoeae MS11

49.291

98.947

0.488
  pilB Vibrio parahaemolyticus RIMD 2210633

50.659

93.158

0.472
  pilB Vibrio campbellii strain DS40M4

50.758

92.632

0.47
  pilB/pilB1 Synechocystis sp. PCC 6803

41.667

90.526

0.377
  pilF Thermus thermophilus HB27

38.559

97.368

0.375
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.228

92.456

0.372

Multiple sequence alignment