Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   ACETWS_RS26960 Genome accession   NZ_CP169073
Coordinates   5978542..5979066 (+) Length   174 a.a.
NCBI ID   WP_310036869.1    Uniprot ID   -
Organism   Delftia lacustris strain ZWP15     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5973542..5984066
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACETWS_RS26940 (ACETWS_26940) sucD 5974532..5975425 (+) 894 WP_016449394.1 succinate--CoA ligase subunit alpha -
  ACETWS_RS26945 (ACETWS_26945) - 5975591..5976283 (+) 693 WP_016449393.1 TerC family protein -
  ACETWS_RS26950 (ACETWS_26950) - 5976578..5977360 (+) 783 WP_133072855.1 PP2C family serine/threonine-protein phosphatase -
  ACETWS_RS26955 (ACETWS_26955) - 5977609..5978319 (+) 711 WP_017405118.1 FHA domain-containing protein -
  ACETWS_RS26960 (ACETWS_26960) pilE 5978542..5979066 (+) 525 WP_310036869.1 pilin Machinery gene
  ACETWS_RS26965 (ACETWS_26965) - 5979044..5980963 (+) 1920 WP_374006313.1 hypothetical protein -
  ACETWS_RS26970 (ACETWS_26970) - 5981186..5981938 (-) 753 WP_374006314.1 SDR family NAD(P)-dependent oxidoreductase -
  ACETWS_RS26975 (ACETWS_26975) - 5981940..5983247 (-) 1308 WP_374006315.1 FAD-dependent oxidoreductase -
  ACETWS_RS26980 (ACETWS_26980) - 5983244..5983657 (-) 414 WP_063327518.1 GtrA family protein -

Sequence

Protein


Download         Length: 174 a.a.        Molecular weight: 17904.45 Da        Isoelectric Point: 9.0399

>NTDB_id=1049792 ACETWS_RS26960 WP_310036869.1 5978542..5979066(+) (pilE) [Delftia lacustris strain ZWP15]
MKRSIQQGFTLIELMIVVAIIGILAAVALPAYQDYTVRAKVSEAVIQATSPKSLISEAFQTDGLSGMVAAATTFNNKPSN
EKATKYVADMTINSANGQITVTLQSGTSAGFPSDVRGTTLIFTPNVQKVALADGKQGAIDWACGSTTASTAGTRGLGAAA
TGTLPAKYAPAECR

Nucleotide


Download         Length: 525 bp        

>NTDB_id=1049792 ACETWS_RS26960 WP_310036869.1 5978542..5979066(+) (pilE) [Delftia lacustris strain ZWP15]
ATGAAGCGTTCCATCCAGCAAGGTTTTACCTTGATCGAATTGATGATCGTTGTGGCGATCATCGGCATTTTGGCAGCCGT
TGCGCTGCCGGCTTATCAGGATTACACGGTGCGAGCGAAAGTCAGCGAAGCTGTAATTCAAGCTACATCTCCAAAGTCTC
TGATCAGCGAAGCTTTCCAGACTGACGGCCTTTCTGGCATGGTGGCAGCGGCCACAACATTCAACAACAAACCTAGCAAC
GAGAAGGCGACCAAGTACGTTGCAGATATGACCATCAATTCTGCAAATGGTCAAATCACTGTTACATTGCAAAGTGGTAC
TAGCGCAGGATTCCCCTCCGATGTGCGTGGAACGACCCTGATCTTTACGCCCAATGTGCAAAAAGTTGCGCTGGCCGATG
GCAAGCAAGGTGCTATCGACTGGGCTTGCGGCTCGACCACTGCATCTACAGCTGGTACTCGCGGGCTGGGTGCAGCTGCA
ACGGGTACTCTGCCTGCTAAGTATGCCCCTGCTGAGTGCCGTTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(37-174)

Prokaryotic N-terminal methylation motif (N_methyl)

(4-29)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

51.872

100

0.557
  pilA Ralstonia pseudosolanacearum GMI1000

42.458

100

0.437
  pilA2 Legionella pneumophila strain ERS1305867

40.909

100

0.414
  pilA2 Legionella pneumophila str. Paris

41.42

97.126

0.402
  comP Acinetobacter baylyi ADP1

38.286

100

0.385
  pilE Neisseria gonorrhoeae strain FA1090

36.723

100

0.374
  pilA/pilAI Pseudomonas stutzeri DSM 10701

36.207

100

0.362

Multiple sequence alignment