Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACEO96_RS02955 Genome accession   NZ_CP168657
Coordinates   600415..602103 (-) Length   562 a.a.
NCBI ID   WP_170899399.1    Uniprot ID   -
Organism   Vibrio anguillarum strain VA1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 595415..607103
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEO96_RS02925 (ACEO96_02925) rplS 596144..596497 (+) 354 WP_010319525.1 50S ribosomal protein L19 -
  ACEO96_RS02930 (ACEO96_02930) yacG 596567..596764 (-) 198 WP_010319526.1 DNA gyrase inhibitor YacG -
  ACEO96_RS02935 (ACEO96_02935) zapD 596854..597594 (-) 741 WP_013857689.1 cell division protein ZapD -
  ACEO96_RS02940 (ACEO96_02940) coaE 597627..598235 (-) 609 WP_064624393.1 dephospho-CoA kinase -
  ACEO96_RS02945 (ACEO96_02945) pilD 598226..599107 (-) 882 WP_170899398.1 A24 family peptidase Machinery gene
  ACEO96_RS02950 (ACEO96_02950) pilC 599146..600372 (-) 1227 WP_047688859.1 type II secretion system F family protein Machinery gene
  ACEO96_RS02955 (ACEO96_02955) pilB 600415..602103 (-) 1689 WP_170899399.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACEO96_RS02960 (ACEO96_02960) - 602108..602563 (-) 456 WP_199485555.1 pilin -
  ACEO96_RS02965 (ACEO96_02965) nadC 602822..603709 (-) 888 WP_094148110.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACEO96_RS02970 (ACEO96_02970) ampD 603834..604385 (+) 552 WP_202599069.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACEO96_RS02975 (ACEO96_02975) fldB 604407..604925 (-) 519 WP_013857680.1 flavodoxin FldB -
  ACEO96_RS02980 (ACEO96_02980) xerD 605078..605986 (+) 909 WP_013857679.1 site-specific tyrosine recombinase XerD -
  ACEO96_RS02985 (ACEO96_02985) - 606000..606773 (+) 774 WP_017045785.1 thioredoxin fold domain-containing protein -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62240.42 Da        Isoelectric Point: 6.3770

>NTDB_id=1044816 ACEO96_RS02955 WP_170899399.1 600415..602103(-) (pilB) [Vibrio anguillarum strain VA1]
MLTNLPAILRQAQILSPTQEHAVIEHIKASGASVPEALLALDFFHPHDLTEQLSTLFGLPDTDLTRYDYPLLCQQLGLRD
LITRYQALPIANNGQTLTIAVSDPTHLQVEDDFRFATGLQVELVLADYKALQAAIRRLYGRTIQGSSSSGKEISQDELAN
LVKLSDDEIQSIEDLSQDDAPVSRFINQILLDAVRKGASDIHFEPYEALYRVRLRCDGILVETQRPSSNLSRRLAARLKI
LSKLDIAERRLPQDGRIKLRLNQETAIDMRVSSLPTLWGEKIVLRLLDSSAANLDIDKLGYNDQQKQLYLAALKKPQGMI
LMTGPTGSGKTVSLYTGLRILNTAEVNISTAEDPVEINLSGINQVQVQPKIGFGFAQALRSFLRQDPDIVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAETVIRLSNMGIEAFNLASSLSLIIAQRLARRLCRHCKIAVVPSMQLQQHFQLLATE
TIYEANPHGCNECTGGYSGRVGIYEVMAFNPQLAEAIMQKASIHQIEQIAKANGMQTLQESGIEKLKNGVTSFKELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=1044816 ACEO96_RS02955 WP_170899399.1 600415..602103(-) (pilB) [Vibrio anguillarum strain VA1]
ATGCTAACCAACCTCCCTGCGATTCTGCGTCAGGCTCAGATATTGAGCCCGACGCAAGAACATGCAGTTATTGAACATAT
CAAAGCGTCTGGTGCGAGTGTGCCAGAAGCTTTGCTCGCTTTGGATTTTTTCCATCCTCATGATCTGACTGAACAGTTAA
GTACCCTTTTTGGCCTACCGGACACCGACTTAACCCGCTACGACTACCCACTGCTCTGCCAACAGCTTGGTTTGCGTGAT
CTCATCACCCGTTACCAAGCACTGCCGATTGCCAATAATGGCCAAACCCTGACTATCGCGGTTTCCGACCCGACCCATTT
ACAAGTGGAAGATGATTTTCGCTTCGCAACAGGCTTACAAGTTGAACTGGTTTTAGCCGACTACAAAGCGCTGCAAGCAG
CCATCCGGCGCTTGTATGGCCGAACAATTCAAGGATCCTCTTCTTCAGGGAAAGAGATCAGCCAAGATGAACTGGCGAAC
TTAGTTAAGCTGTCTGATGATGAGATTCAATCGATTGAAGACCTCAGCCAAGATGACGCCCCAGTCAGTCGCTTTATTAA
CCAAATCCTGCTTGATGCGGTGCGAAAAGGCGCATCCGATATCCATTTCGAACCGTACGAAGCGCTTTACCGAGTACGAT
TGCGCTGTGATGGTATTTTGGTGGAAACGCAGCGTCCCTCAAGCAACTTAAGTCGCCGGCTTGCCGCGCGGCTTAAAATT
TTATCCAAACTCGATATTGCCGAACGGCGCTTACCACAAGATGGACGAATTAAACTGCGGTTGAATCAAGAGACCGCCAT
CGATATGCGCGTTTCCAGCCTCCCTACTTTATGGGGTGAAAAAATCGTACTGCGCCTACTTGATAGCAGTGCTGCTAACT
TAGATATCGACAAATTGGGTTATAACGACCAGCAAAAACAACTCTACTTAGCTGCGCTGAAAAAACCACAAGGGATGATT
TTAATGACGGGCCCGACGGGCAGTGGAAAAACCGTCTCGCTCTATACGGGGCTACGCATTTTAAATACAGCTGAAGTGAA
CATTTCCACCGCTGAAGATCCGGTTGAAATTAACTTATCCGGCATCAACCAAGTTCAAGTGCAGCCAAAAATTGGCTTTG
GCTTTGCGCAAGCATTACGCTCTTTCTTACGGCAAGATCCCGACATTGTGATGGTTGGCGAAATCCGCGATTTAGAAACT
GCCGAAATCGCCGTGAAAGCGGCACAAACCGGGCATTTAGTTCTTTCTACCTTGCATACTAACTCCGCCGCCGAAACGGT
AATTCGCTTGAGTAATATGGGAATTGAAGCGTTTAACTTAGCCTCTTCACTCAGTTTAATTATTGCCCAGCGTTTAGCTC
GTCGCCTTTGTCGGCACTGCAAAATTGCCGTCGTGCCCTCAATGCAGTTGCAGCAACATTTTCAGCTGCTAGCGACAGAA
ACCATTTATGAAGCAAACCCGCATGGTTGCAACGAATGCACAGGGGGCTATTCAGGTCGAGTCGGCATTTATGAAGTGAT
GGCGTTTAACCCACAGCTGGCTGAAGCGATTATGCAAAAAGCAAGCATTCATCAAATAGAGCAGATCGCGAAAGCCAACG
GAATGCAGACACTACAAGAATCAGGCATTGAAAAGCTCAAAAACGGCGTAACCAGCTTCAAAGAGCTGCAACGCGTGCTC
TATTTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

83.096

100

0.831
  pilB Vibrio campbellii strain DS40M4

74.377

100

0.744
  pilB Vibrio parahaemolyticus RIMD 2210633

72.954

100

0.73
  pilB Acinetobacter baumannii D1279779

51.923

92.527

0.48
  pilB Legionella pneumophila strain ERS1305867

51.758

91.103

0.472
  pilB Acinetobacter baylyi ADP1

50.38

93.594

0.472
  pilF Neisseria gonorrhoeae MS11

44.248

100

0.445
  pilB/pilB1 Synechocystis sp. PCC 6803

36.149

100

0.381
  pilF Thermus thermophilus HB27

37.61

100

0.381

Multiple sequence alignment