Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACEN3H_RS16725 Genome accession   NZ_CP168231
Coordinates   3588186..3589898 (+) Length   570 a.a.
NCBI ID   WP_373358131.1    Uniprot ID   -
Organism   Acinetobacter lactucae strain 2024CK-01095     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3583186..3594898
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEN3H_RS16690 (ACEN3H_16690) nusA 3584074..3585558 (-) 1485 WP_002115134.1 transcription termination factor NusA -
  ACEN3H_RS16695 (ACEN3H_16695) rimP 3585596..3586120 (-) 525 WP_016144169.1 ribosome maturation factor RimP -
  ACEN3H_RS16715 (ACEN3H_16715) secG 3586758..3587087 (-) 330 WP_002115012.1 preprotein translocase subunit SecG -
  ACEN3H_RS16720 (ACEN3H_16720) tpiA 3587100..3587894 (-) 795 WP_368678135.1 triose-phosphate isomerase -
  ACEN3H_RS16725 (ACEN3H_16725) pilB 3588186..3589898 (+) 1713 WP_373358131.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACEN3H_RS16730 (ACEN3H_16730) pilC 3589926..3591152 (+) 1227 WP_057104884.1 type II secretion system F family protein Machinery gene
  ACEN3H_RS16735 (ACEN3H_16735) pilD 3591152..3592012 (+) 861 WP_373358132.1 A24 family peptidase Machinery gene
  ACEN3H_RS16740 (ACEN3H_16740) coaE 3592014..3592610 (+) 597 WP_062849621.1 dephospho-CoA kinase -
  ACEN3H_RS16745 (ACEN3H_16745) - 3592607..3593521 (-) 915 WP_373358133.1 DMT family transporter -
  ACEN3H_RS16750 (ACEN3H_16750) rlmB 3593557..3594306 (-) 750 WP_005079348.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  ACEN3H_RS16755 (ACEN3H_16755) - 3594409..3594735 (-) 327 WP_002115102.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63613.03 Da        Isoelectric Point: 5.0932

>NTDB_id=1043331 ACEN3H_RS16725 WP_373358131.1 3588186..3589898(+) (pilB) [Acinetobacter lactucae strain 2024CK-01095]
MSVIHTSPKFSGFFRRLVEEKHVSAATMQTALDAAKRAKRDTVAYLIEEVHLSPSLLAETISIEFAEPYFDLDVYDTSQI
PKDLVDQKLILKHRVLPLIQRGQILYVATSNPSNIEAIDAIRFNSKLMVEPVIVEHHKLEKILTQQFTEESSFEFSDEEF
DLDVDLEAPPSQEDEEETPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEDQKALFLEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFSAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCTQCKIPADIPKQSLLEMGF
TEQDLTHPDFQVFQPVGCTECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=1043331 ACEN3H_RS16725 WP_373358131.1 3588186..3589898(+) (pilB) [Acinetobacter lactucae strain 2024CK-01095]
ATGTCAGTCATACATACATCTCCAAAATTTTCGGGGTTTTTTCGGCGATTAGTCGAAGAAAAACATGTGTCGGCAGCGAC
CATGCAAACAGCATTAGATGCAGCAAAGAGAGCTAAACGGGATACGGTTGCGTACCTGATTGAAGAGGTTCATCTCTCCC
CTTCTTTATTAGCTGAAACTATTTCTATTGAATTTGCTGAACCATATTTTGATCTGGACGTTTACGATACCAGCCAAATT
CCTAAAGATTTGGTTGACCAAAAACTGATTTTAAAACATCGGGTCTTACCTCTCATACAAAGAGGACAGATTTTATATGT
CGCGACCAGCAATCCAAGTAATATTGAAGCGATTGATGCCATTCGCTTCAATAGTAAGCTCATGGTTGAACCAGTTATTG
TCGAGCACCATAAGCTTGAGAAAATTCTGACGCAACAATTTACCGAAGAAAGCAGTTTCGAGTTTAGTGATGAAGAGTTT
GATCTTGATGTAGACCTTGAAGCACCACCATCACAAGAGGATGAGGAAGAAACACCCCAAGGCGATGAAGCACCTATTGT
TAAATATATTAATAAATTACTCATAGATGCGATTCGTATGGGGGCTTCAGACTTACATTTTGAACCCTATGAAAAATCAT
ATCGGGTCCGTTACCGAGTCGACGGCGTGTTGCGTCAGATTGCAAATCCGCCTTTACAATTGGCTAACCGTTTGGCTTCA
CGTTTAAAAGTCATGTCTCAAATGGATATTTCCGAAAAACGGGTGCCTCAAGATGGTCGTATCAAACTTAAGCTCTCAAA
AACTAAAGCAATCGATTTTCGTGTGAACTCACTTCCAACCTTGTTTGGTGAAAAACTGGTTCTGCGTATTCTCGATCCAT
CTAGCGCGATGCTCGGAATTGATGCTTTAGGATATGAAGAAGATCAGAAAGCTCTTTTTTTAGAAGCACTGGATAAGCCC
CAAGGTATGCTTCTAATTACAGGCCCTACTGGCTCTGGTAAAACGGTATCTTTATATACAGGCTTAAATATTTTAAATAC
TGAAAGCTCTAATATTTCCACTGCCGAAGATCCAGTCGAAATCAACCTTGAAGGTATTAATCAGGTTAACGTTAACCCGA
AAGTAGGACTGACTTTTTCTGCTGCACTTAAATCATTTTTACGTCAGGACCCTGACATTATTATGGTCGGTGAGATTCGT
GACTTAGAAACAGCTGAAATTGCGATTAAAGCTGCACAAACTGGTCACATGGTAATGTCCACACTGCATACCAATAGTGC
TCCAGAAACACTTACTCGCCTACGTAATATGGGTGTTCCATCCTTTAATATCGCAACTTCTGTCAATCTTGTGATTGCTC
AACGTTTAGCACGTCGGCTATGCACTCAGTGTAAAATACCTGCCGACATTCCTAAACAGAGCCTATTAGAAATGGGCTTT
ACCGAACAAGATCTGACTCATCCCGATTTTCAAGTTTTTCAACCTGTGGGTTGTACTGAGTGTCGGGAAGGCTATAAAGG
TCGAGTAGGTATTTACGAGGTAATGAAAGTTACACCTGAAATTTCCAAGATTATTATGGAAGATGGCAATGCTTTAGAAA
TCGCTGCTGCTTCTGAAAAACTGGGGTTTAATAATCTGCGTCGTTCAGGATTAAAGAAAGTCATGCAAGGTGTTACTTCT
TTACAGGAAGTCAACCGCGTGACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

95.439

100

0.954
  pilB Acinetobacter baylyi ADP1

78.246

100

0.782
  pilB Legionella pneumophila strain ERS1305867

51.675

99.474

0.514
  pilF Neisseria gonorrhoeae MS11

49.822

98.596

0.491
  pilB Vibrio parahaemolyticus RIMD 2210633

51.321

92.982

0.477
  pilB Vibrio campbellii strain DS40M4

50.766

91.579

0.465
  pilB Vibrio cholerae strain A1552

50.482

91.053

0.46
  pilB/pilB1 Synechocystis sp. PCC 6803

35.831

100

0.386
  pilF Thermus thermophilus HB27

36.833

98.596

0.363

Multiple sequence alignment