Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACEN3N_RS01685 Genome accession   NZ_CP168228
Coordinates   364802..366514 (-) Length   570 a.a.
NCBI ID   WP_151961536.1    Uniprot ID   -
Organism   Acinetobacter bereziniae strain 2024CK-01176     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 359802..371514
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEN3N_RS01655 (ACEN3N_01655) - 359824..360147 (+) 324 WP_004718357.1 pyrimidine/purine nucleoside phosphorylase -
  ACEN3N_RS01660 (ACEN3N_01660) rlmB 360291..361043 (+) 753 WP_373350680.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  ACEN3N_RS01665 (ACEN3N_01665) - 361084..362004 (+) 921 WP_151961532.1 DMT family transporter -
  ACEN3N_RS01670 (ACEN3N_01670) coaE 362051..362650 (-) 600 WP_335990246.1 dephospho-CoA kinase -
  ACEN3N_RS01675 (ACEN3N_01675) pilD 362676..363548 (-) 873 WP_151961534.1 A24 family peptidase Machinery gene
  ACEN3N_RS01680 (ACEN3N_01680) pilC 363548..364774 (-) 1227 WP_151961535.1 type II secretion system F family protein Machinery gene
  ACEN3N_RS01685 (ACEN3N_01685) pilB 364802..366514 (-) 1713 WP_151961536.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACEN3N_RS01690 (ACEN3N_01690) tpiA 366793..367587 (+) 795 WP_151780909.1 triose-phosphate isomerase -
  ACEN3N_RS01695 (ACEN3N_01695) secG 367597..367938 (+) 342 WP_004826367.1 preprotein translocase subunit SecG -
  ACEN3N_RS01720 (ACEN3N_01720) rimP 368732..369256 (+) 525 WP_004826368.1 ribosome maturation factor RimP -
  ACEN3N_RS01725 (ACEN3N_01725) nusA 369291..370775 (+) 1485 WP_004826370.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63271.80 Da        Isoelectric Point: 5.0163

>NTDB_id=1043264 ACEN3N_RS01685 WP_151961536.1 364802..366514(-) (pilB) [Acinetobacter bereziniae strain 2024CK-01176]
MSMQNGSPKFSGYIRRLVDEGFLSAQQMKDALTSAQKSKTHLVPYLIEKLAISPLLIAEMISSEFGEPIFDLDSFNPEML
HKDIVDAGLISKHGVIPLVQRGNILYVATSDPTNIEALDAIRFSCGLNIEPIIVEQHKLEKIIAKHFTVEESFNFGDEDF
DVDFIATDENQPAELDEETAEEEAPIVKYVNKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRTITNPPLQLANRLAS
RLKVMSQMDISEKRIPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEDQKALFMEALEKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNLKAGLTFSVALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCNHCKAPIEIPKQSLLELGF
TEQDLNHPDLQIFQPVGCSECREGYKGRVGIYEVMKVTPEISKIIMEDGNALQIAAASEKLGFDNLRRSGLKKVMNGLTS
LQEINRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=1043264 ACEN3N_RS01685 WP_151961536.1 364802..366514(-) (pilB) [Acinetobacter bereziniae strain 2024CK-01176]
ATGTCTATGCAAAATGGTTCACCTAAGTTTTCGGGTTATATCCGTCGCTTAGTCGATGAAGGCTTTTTATCTGCTCAGCA
AATGAAAGATGCCTTAACAAGCGCTCAAAAAAGTAAAACCCATTTGGTGCCTTACTTGATTGAGAAATTAGCGATCTCAC
CATTGCTGATCGCTGAAATGATCTCCTCCGAGTTCGGCGAGCCTATTTTTGATCTAGACAGTTTTAACCCTGAAATGTTG
CACAAGGACATTGTGGACGCCGGTTTGATCAGCAAACATGGGGTGATTCCATTGGTGCAACGCGGTAATATCTTATATGT
TGCCACCAGCGACCCCACCAATATCGAGGCACTTGATGCGATTCGTTTTAGTTGTGGTCTAAACATTGAGCCAATCATCG
TTGAACAACATAAATTAGAAAAAATTATAGCCAAACATTTTACTGTAGAAGAAAGCTTTAATTTTGGCGATGAAGATTTT
GATGTTGATTTTATTGCAACGGATGAAAATCAACCCGCCGAACTTGATGAAGAAACTGCTGAAGAAGAAGCGCCGATTGT
TAAATATGTCAATAAACTGTTGATTGATGCCATTCGAATGGGCGCCTCGGACTTACATTTTGAACCTTATGAAAAAAGTT
ATCGTGTTCGTTATCGTGTAGATGGTGTACTCAGAACTATCACCAATCCCCCTCTGCAATTGGCGAATCGTTTAGCATCC
CGTTTGAAAGTCATGTCGCAAATGGATATTTCAGAGAAACGTATTCCTCAAGATGGGCGGATCAAACTTAAACTGTCTAA
AACCAAAGCTATTGATTTTCGTGTTAACTCACTGCCAACATTGTTTGGTGAAAAATTAGTTCTGCGTATTCTTGATCCTT
CCAGTGCCATGTTAGGGATTGATGCGTTGGGCTATGAAGAAGACCAAAAAGCTTTATTTATGGAAGCACTAGAAAAACCA
CAAGGTATGTTATTGATTACCGGTCCAACAGGTTCTGGTAAAACAGTATCGTTGTATACAGGCTTAAATATCCTCAATAC
CGAAAGTTCCAATATTTCAACCGCAGAAGACCCTGTTGAGATTAACTTGGAGGGGATTAACCAAGTAAACGTCAATCTAA
AAGCAGGATTGACCTTTTCGGTCGCGCTGAAATCATTTCTTCGTCAAGATCCAGATATTATCATGGTCGGGGAGATTCGA
GACCTTGAAACGGCTGAAATTGCAATCAAAGCAGCCCAAACAGGTCATATGGTGATGTCCACACTGCATACCAACAGTGC
ACCTGAAACCTTAACCCGTTTGCGCAATATGGGCGTGCCATCCTTTAATATTGCCACATCGGTCAATCTTGTGATTGCAC
AACGTCTTGCTCGGCGCTTATGCAACCACTGTAAAGCACCTATTGAAATTCCGAAACAAAGTTTGTTAGAACTGGGCTTT
ACGGAACAAGACTTAAACCATCCTGATTTACAGATTTTTCAGCCTGTGGGTTGTTCTGAATGTAGAGAAGGTTATAAAGG
ACGAGTCGGTATTTATGAGGTGATGAAAGTAACACCTGAAATTTCAAAAATTATCATGGAAGATGGCAATGCACTGCAAA
TTGCAGCAGCATCTGAAAAATTGGGGTTTGATAATTTGCGACGTTCGGGTTTAAAAAAGGTTATGAATGGTTTAACGTCT
TTACAAGAAATTAACCGCGTAACCAGCGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

80.07

100

0.804
  pilB Acinetobacter baylyi ADP1

75.088

100

0.751
  pilB Legionella pneumophila strain ERS1305867

51.215

100

0.518
  pilF Neisseria gonorrhoeae MS11

49.288

98.596

0.486
  pilB Vibrio parahaemolyticus RIMD 2210633

48.654

97.719

0.475
  pilB Vibrio cholerae strain A1552

48.468

97.368

0.472
  pilB Vibrio campbellii strain DS40M4

49.712

91.404

0.454
  pilF Thermus thermophilus HB27

37.018

100

0.37
  pilB/pilB1 Synechocystis sp. PCC 6803

34.095

100

0.367

Multiple sequence alignment