Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   R8721_RS07845 Genome accession   NZ_AP028321
Coordinates   1671887..1673026 (-) Length   379 a.a.
NCBI ID   WP_017766703.1    Uniprot ID   -
Organism   Aeromonas dhakensis strain RYU-Ah62     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1666887..1678026
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R8721_RS07820 (Ri1_14850) - 1666893..1667120 (+) 228 WP_005302641.1 FeoA family protein -
  R8721_RS07825 (Ri1_14860) feoB 1667117..1669390 (+) 2274 WP_010633536.1 Fe(2+) transporter permease subunit FeoB -
  R8721_RS07830 (Ri1_14870) - 1669387..1669629 (+) 243 WP_005302637.1 FeoC-like transcriptional regulator -
  R8721_RS07835 (Ri1_14880) - 1669742..1670221 (-) 480 WP_010633537.1 WbuC family cupin fold metalloprotein -
  R8721_RS07840 (Ri1_14890) glgC 1670357..1671628 (-) 1272 WP_010633538.1 glucose-1-phosphate adenylyltransferase -
  R8721_RS07845 (Ri1_14900) pilU 1671887..1673026 (-) 1140 WP_017766703.1 type IVa pilus ATPase TapW Machinery gene
  R8721_RS07850 (Ri1_14910) - 1673086..1673442 (-) 357 WP_005302630.1 DMT family protein -
  R8721_RS07855 (Ri1_14920) - 1673556..1674287 (+) 732 WP_024942579.1 ABC transporter substrate-binding protein -
  R8721_RS07860 (Ri1_14930) btuC 1674387..1675394 (+) 1008 WP_010633541.1 vitamin B12 ABC transporter permease BtuC -
  R8721_RS07865 (Ri1_14940) - 1675381..1676166 (+) 786 WP_010633542.1 ATP-binding cassette domain-containing protein -
  R8721_RS07870 (Ri1_14950) - 1676246..1677307 (-) 1062 WP_010633543.1 3-deoxy-7-phosphoheptulonate synthase -
  R8721_RS07875 (Ri1_14960) - 1677464..1677844 (+) 381 WP_010633544.1 diacylglycerol kinase -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42171.19 Da        Isoelectric Point: 6.2561

>NTDB_id=104193 R8721_RS07845 WP_017766703.1 1671887..1673026(-) (pilU) [Aeromonas dhakensis strain RYU-Ah62]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGGLRPLSETPLEPGEVASIADAIMDGEQKQQFEKELEMNLAISLPQIGR
FRINIFKQRNEVSLVARNIKTEIPRFEDLKLPTVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAAVEIMLGTHTIRDMVKRGEFGGLKEVMEKSKALGMVTFDSAL
FDLVVEGVIDEEEAVKNADSANNLRLKIKLWKEKGQIASSSDATGWSLEPTKDEKDDLF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=104193 R8721_RS07845 WP_017766703.1 1671887..1673026(-) (pilU) [Aeromonas dhakensis strain RYU-Ah62]
ATGGAATTGCGAGACATGCTACAGATCCTGGCCAAGCAGGACGGCTCAGACCTCTATCTGTCGACCGGCGCGCCCCCCTG
TGCCAAGTTCAACGGCGGCCTGCGCCCCTTGAGCGAGACGCCGCTCGAGCCCGGCGAGGTGGCCAGCATAGCCGACGCCA
TCATGGACGGCGAGCAGAAGCAGCAGTTTGAAAAGGAGCTGGAGATGAACCTCGCCATCTCCCTGCCCCAGATAGGGCGC
TTTCGGATCAACATCTTCAAGCAGCGCAACGAAGTCTCCCTGGTGGCGCGCAACATCAAGACCGAGATCCCTCGCTTCGA
GGACTTGAAACTGCCCACAGTGCTGCTCGACACCATCATGGAGAAACGGGGGCTGGTGCTGTTCGTCGGCGGCACCGGTT
CGGGCAAATCCACCTCGCTCGCGGCCCTTATCGACCATCGCAACCGCAACAGCGGCGGCCACATCATCACCATCGAGGAT
CCGGTGGAGTTCGTGCACCGCCACCGCAAGAGCATCATCAACCAGCGAGAGGTGGGGGTGGATACCCGCAGCTTCCACGC
CGCCCTGAAAAACACCCTGCGCCAGGCGCCGGATGTGATCCTTATCGGCGAAATTCGCGATCGCGAAACCATGGAGCACG
CACTGGCCTTCTCGGAAACCGGCCACCTGGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGGAAGAGCGCCGGCCCCAGCTGCTCAACGATCTTGGCAACAACCTCAAGGCGTTTGTCTCCCAGCG
GCTGGTGAAAACCACTGATGGCGGCCGCCGGGCCGCGGTAGAGATCATGCTGGGCACCCACACCATCCGCGACATGGTCA
AGCGCGGCGAGTTTGGCGGCCTCAAGGAGGTGATGGAGAAGTCCAAGGCCCTCGGCATGGTCACCTTCGACAGCGCCCTG
TTCGATCTGGTGGTGGAAGGGGTGATCGACGAGGAGGAGGCGGTGAAGAACGCCGATTCGGCCAACAACCTGCGCCTCAA
GATCAAGCTGTGGAAGGAGAAGGGCCAGATCGCCAGCAGCAGCGATGCCACCGGCTGGAGCCTGGAGCCTACCAAGGACG
AAAAGGACGATCTGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

53.867

98.945

0.533
  pilU Acinetobacter baylyi ADP1

52.958

93.668

0.496
  pilU Vibrio cholerae strain A1552

50

92.348

0.462
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.793

93.14

0.38
  pilT Pseudomonas aeruginosa PAK

40.407

90.765

0.367
  pilT Legionella pneumophila strain ERS1305867

41.317

88.127

0.364
  pilT Legionella pneumophila strain Lp02

41.317

88.127

0.364
  pilT Pseudomonas stutzeri DSM 10701

39.826

90.765

0.361

Multiple sequence alignment