Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ABRP31_RS25015 Genome accession   NZ_CP167995
Coordinates   5553160..5555262 (-) Length   700 a.a.
NCBI ID   WP_349972186.1    Uniprot ID   -
Organism   Pseudomonas caspiana strain WHRI 7043     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5548160..5560262
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABRP31_RS25000 (ABRP31_025000) - 5549775..5551394 (-) 1620 WP_349972190.1 AAA family ATPase -
  ABRP31_RS25005 (ABRP31_025005) aroB 5551404..5552507 (-) 1104 WP_349972188.1 3-dehydroquinate synthase -
  ABRP31_RS25010 (ABRP31_025010) aroK 5552637..5553155 (-) 519 WP_087271137.1 shikimate kinase AroK -
  ABRP31_RS25015 (ABRP31_025015) pilQ 5553160..5555262 (-) 2103 WP_349972186.1 type IV pilus secretin PilQ Machinery gene
  ABRP31_RS25020 (ABRP31_025020) pilP 5555319..5555846 (-) 528 WP_087271131.1 type 4a pilus biogenesis lipoprotein PilP -
  ABRP31_RS25025 (ABRP31_025025) pilO 5555843..5556466 (-) 624 WP_349972185.1 type 4a pilus biogenesis protein PilO -
  ABRP31_RS25030 (ABRP31_025030) - 5556463..5557029 (-) 567 WP_349972184.1 PilN domain-containing protein -
  ABRP31_RS25035 (ABRP31_025035) pilM 5557029..5558093 (-) 1065 WP_087271122.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 700 a.a.        Molecular weight: 75655.12 Da        Isoelectric Point: 5.0005

>NTDB_id=1040994 ABRP31_RS25015 WP_349972186.1 5553160..5555262(-) (pilQ) [Pseudomonas caspiana strain WHRI 7043]
MTRILSTIGVSLWIAMISPILQAATLKTLDVAALPGDRIELKLTFDAPVPAPKGYTTEQPARIALDLPGVSSQLATKSRE
LGSGNARSVVVVEAKDRTRIIINMTTLAPYSSRVDGNNLFVVIGQGAAAAQASQPTTALGAARVAVPPPAPAKPKAYAPV
GKAIRNVDFQRGELGEGNVIIDLTDPGVAPDIQEQGGRIRVDFAKTQLPEKLRVRLDVKDFATPVQFVNASATGDKASIT
IEPSGAFDYSAYQTEGKLTISVRPLTNEDLDRRNAEKLVYTGEKLSLNFQDIDVRSVLQLIADFTSLNLVASDTVQGGIT
LRLQNVPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESLKQIAELAPLRRELLQVNYAKAADIAKLFQSV
TSAESKVDERGSITVDERTNNIIAYQTQDRLDELRRIVSQLDIPVRQVMIEARIVEANVGYDKALGVRWAGDTNRNNNHK
WSGYGLDDNGDEAGNTGDALTSNVPFVDLGAADATSGIGIAFMTNNTLLDLELSAMERTGNGEIVSQPKVVTSDKETAKI
LKGTEIPYQESSSSGATTVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNTVLGVPPIRKNEVNAKVLVADGETI
VIGGVFSNTQSKVVDKVPFLGDVPYVGRLFRRDVVSEAKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2103 bp        

>NTDB_id=1040994 ABRP31_RS25015 WP_349972186.1 5553160..5555262(-) (pilQ) [Pseudomonas caspiana strain WHRI 7043]
ATGACCAGGATTCTTTCAACTATCGGTGTATCGCTATGGATTGCGATGATTTCGCCGATTCTTCAGGCCGCCACTCTCAA
AACACTGGATGTGGCAGCGTTGCCTGGAGACCGGATCGAGCTGAAGCTGACCTTTGACGCTCCGGTACCTGCTCCGAAGG
GCTATACCACGGAACAACCGGCGCGCATCGCGCTGGACTTGCCCGGCGTGAGCAGTCAGCTGGCGACCAAAAGCCGTGAG
TTGGGCAGTGGCAATGCTCGCAGTGTGGTCGTGGTCGAAGCCAAGGATCGTACTCGGATCATTATCAATATGACGACCCT
GGCGCCCTACAGCTCGCGTGTTGATGGCAATAATCTGTTTGTCGTGATCGGTCAGGGGGCCGCAGCGGCTCAGGCATCGC
AGCCGACCACTGCGCTGGGCGCCGCGCGGGTTGCCGTGCCACCACCAGCTCCGGCAAAGCCCAAGGCCTACGCGCCTGTA
GGCAAGGCGATCAGGAACGTTGATTTTCAGCGAGGCGAGTTGGGTGAAGGGAATGTGATCATCGATTTGACTGATCCCGG
TGTCGCCCCAGATATTCAGGAGCAGGGCGGCAGGATTCGTGTCGATTTTGCGAAGACTCAATTACCTGAGAAACTGCGGG
TGCGACTGGACGTCAAGGATTTCGCCACCCCGGTGCAGTTCGTCAACGCCAGTGCTACGGGTGATAAGGCCAGTATCACC
ATCGAGCCTTCCGGGGCCTTTGACTACTCGGCCTATCAGACCGAAGGCAAGCTAACCATTAGCGTGCGCCCCTTGACCAA
TGAAGATCTGGATCGCCGCAACGCGGAGAAACTGGTCTACACCGGTGAAAAACTGTCGCTCAACTTTCAGGACATCGACG
TGCGTTCGGTGCTGCAGCTGATCGCCGATTTCACCAGCCTCAACCTGGTCGCCAGCGACACGGTTCAGGGCGGCATTACC
TTGCGTCTGCAGAACGTGCCTTGGGATCAAGCGCTGGATCTGGTGTTGAAAACCAAAGGCCTGGACAAGCGCAAGATTGG
CAATGTGTTGCTGGTGGCACCTGCCGACGAAATTGCCGCCCGTGAGCGTCAGGAGCTGGAATCTCTCAAGCAGATCGCCG
AGCTGGCACCACTGCGCCGCGAATTGCTGCAAGTCAACTACGCCAAAGCGGCGGACATTGCCAAGCTGTTCCAGTCGGTG
ACCAGTGCCGAGTCCAAAGTCGATGAGCGTGGCTCGATCACGGTGGATGAGCGTACCAACAACATCATTGCCTACCAGAC
TCAGGATCGTCTGGACGAGCTGCGCCGCATCGTTTCCCAACTGGATATTCCGGTGCGTCAGGTGATGATCGAGGCACGTA
TCGTTGAGGCCAACGTGGGTTACGACAAAGCACTTGGGGTTCGCTGGGCGGGTGACACCAACCGCAACAACAATCACAAG
TGGAGTGGTTACGGTCTGGACGACAACGGCGACGAGGCGGGCAACACCGGTGACGCACTGACCTCCAACGTGCCGTTCGT
CGACCTGGGCGCGGCGGACGCTACGTCTGGTATTGGTATCGCGTTCATGACCAATAACACGTTGCTGGACCTTGAGCTGA
GCGCCATGGAACGGACCGGTAACGGCGAGATCGTCTCTCAGCCCAAGGTCGTCACCTCCGACAAGGAGACCGCGAAAATT
CTGAAAGGCACCGAGATTCCTTATCAGGAGTCCAGCTCCAGTGGTGCAACCACCGTGTCCTTCAAAGAGGCTTCGCTGTC
GTTGGAGGTTACTCCGCAGATCACCCCGGACAATCGCATCATCATGGAAGTCAAAGTCACCAAGGATGAGCCGGATTACC
TCAATACCGTGCTCGGTGTACCGCCCATTCGCAAAAACGAAGTCAACGCCAAGGTGCTGGTTGCCGACGGCGAAACCATC
GTGATCGGTGGTGTGTTCTCCAATACGCAAAGTAAAGTTGTCGATAAGGTGCCATTTCTCGGCGATGTGCCGTATGTTGG
CCGCCTTTTCCGGCGCGATGTGGTCTCGGAGGCAAAATCCGAGCTGCTGGTGTTCCTCACTCCGCGTATCATGAACAACC
AGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(308-354)

AMIN domain (AMIN)

(38-122)

Bacterial type II/III secretion system short domain (Secretin_N)

(382-447)

Bacterial type II and III secretion system protein (Secretin)

(535-691)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.546

100

0.759
  comQ Acinetobacter baylyi ADP1

41.335

100

0.416
  pilQ Acinetobacter baumannii strain A118

38.525

100

0.403
  pilQ Acinetobacter baumannii D1279779

38.525

100

0.403
  pilQ Legionella pneumophila strain Lp02

38.832

97.857

0.38
  pilQ Legionella pneumophila strain ERS1305867

38.938

96.857

0.377

Multiple sequence alignment