Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACDK45_RS09380 Genome accession   NZ_CP167879
Coordinates   1878521..1878970 (-) Length   149 a.a.
NCBI ID   WP_044364865.1    Uniprot ID   A0AAJ8WBN7
Organism   Haemophilus influenzae strain NTHi52     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1873521..1883970
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACDK45_RS09360 (ACDK45_09360) rho 1873909..1875170 (+) 1262 Protein_1785 transcription termination factor Rho -
  ACDK45_RS09365 (ACDK45_09365) pilD 1875224..1875916 (-) 693 WP_038440789.1 A24 family peptidase Machinery gene
  ACDK45_RS09370 (ACDK45_09370) pilC 1875913..1877133 (-) 1221 WP_011271971.1 type II secretion system F family protein Machinery gene
  ACDK45_RS09375 (ACDK45_09375) pilB 1877130..1878524 (-) 1395 WP_044364868.1 GspE/PulE family protein Machinery gene
  ACDK45_RS09380 (ACDK45_09380) pilA 1878521..1878970 (-) 450 WP_044364865.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  ACDK45_RS09385 (ACDK45_09385) ampD 1879085..1879639 (+) 555 WP_044364862.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACDK45_RS09390 (ACDK45_09390) corC 1880272..1881171 (+) 900 WP_044364861.1 CNNM family magnesium/cobalt transport protein CorC -
  ACDK45_RS09395 (ACDK45_09395) lnt 1881194..1882723 (+) 1530 WP_080334596.1 apolipoprotein N-acyltransferase -
  ACDK45_RS09400 (ACDK45_09400) rsmE 1882773..1883510 (+) 738 WP_374930614.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15541.88 Da        Isoelectric Point: 8.4623

>NTDB_id=1040099 ACDK45_RS09380 WP_044364865.1 1878521..1878970(-) (pilA) [Haemophilus influenzae strain NTHi52]
MKLTTQQTLKKGFTLIELMIVIAIIAILATIAIPSYQNYTKKAAVSELLQASAPYKADVELCVYSTNETTNCTGGKNGIA
ADITTAKGYVASVKTQSGGITVKGNGTLANMEYILQATGNAATGVTWTTTCKGTDASLFPANFCGSVTQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=1040099 ACDK45_RS09380 WP_044364865.1 1878521..1878970(-) (pilA) [Haemophilus influenzae strain NTHi52]
ATGAAATTAACAACACAGCAAACCTTGAAAAAAGGTTTTACATTAATCGAGCTAATGATTGTGATTGCAATTATTGCTAT
TTTAGCCACTATCGCAATTCCTTCTTATCAAAATTATACCAAAAAAGCTGCGGTATCCGAATTACTGCAAGCTTCTGCGC
CTTATAAGGCTGATGTGGAATTATGTGTATATAGCACAAATGAAACAACAAACTGTACGGGTGGAAAAAATGGTATTGCA
GCAGATATAACCACAGCAAAAGGCTATGTAGCCTCAGTGAAAACGCAATCAGGCGGCATTACAGTAAAAGGGAATGGCAC
ATTGGCAAATATGGAATATATTTTGCAAGCTACAGGTAATGCTGCAACAGGTGTAACTTGGACAACAACCTGCAAAGGAA
CGGATGCCTCTTTATTTCCTGCAAATTTTTGCGGAAGTGTCACACAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(10-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

95.973

100

0.96
  pilA Haemophilus influenzae Rd KW20

95.302

100

0.953
  pilA Glaesserella parasuis strain SC1401

54.362

100

0.544
  pilA2 Legionella pneumophila strain ERS1305867

40.136

98.658

0.396
  pilA Vibrio campbellii strain DS40M4

41.135

94.631

0.389
  pilA2 Legionella pneumophila str. Paris

40.288

93.289

0.376

Multiple sequence alignment