Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACCQ09_RS06835 Genome accession   NZ_CP167854
Coordinates   1625630..1626760 (+) Length   376 a.a.
NCBI ID   WP_040940941.1    Uniprot ID   -
Organism   Xanthomonas sp. NCPPB 2586     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1620630..1631760
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACCQ09_RS06810 (ACCQ09_06810) - 1620897..1621415 (-) 519 WP_228428003.1 M23 family metallopeptidase -
  ACCQ09_RS06815 (ACCQ09_06815) - 1621631..1622053 (-) 423 WP_040940938.1 HU family DNA-binding protein -
  ACCQ09_RS06820 (ACCQ09_06820) proC 1622598..1623458 (-) 861 WP_040940939.1 pyrroline-5-carboxylate reductase -
  ACCQ09_RS06825 (ACCQ09_06825) - 1623469..1624182 (-) 714 WP_040940940.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACCQ09_RS06830 (ACCQ09_06830) pilT 1624470..1625507 (+) 1038 WP_003489919.1 type IV pilus twitching motility protein PilT Machinery gene
  ACCQ09_RS06835 (ACCQ09_06835) pilU 1625630..1626760 (+) 1131 WP_040940941.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACCQ09_RS06840 (ACCQ09_06840) - 1627041..1627715 (+) 675 WP_014508435.1 YitT family protein -
  ACCQ09_RS06845 (ACCQ09_06845) - 1627729..1628628 (-) 900 WP_040940942.1 DUF72 domain-containing protein -
  ACCQ09_RS06850 (ACCQ09_06850) - 1628851..1630113 (-) 1263 WP_080640253.1 hypothetical protein -
  ACCQ09_RS06855 (ACCQ09_06855) - 1630110..1630649 (-) 540 WP_012437807.1 DNA-3-methyladenine glycosylase I -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41966.24 Da        Isoelectric Point: 6.8287

>NTDB_id=1039881 ACCQ09_RS06835 WP_040940941.1 1625630..1626760(+) (pilU) [Xanthomonas sp. NCPPB 2586]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVHGKISPITQAPLTAQQSRDLVLNVMTPSQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGRGRRVAMEIMLGTPLVQDYIRDGEIHKLKEIMKESTNLGMRTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLSQGLDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=1039881 ACCQ09_RS06835 WP_040940941.1 1625630..1626760(+) (pilU) [Xanthomonas sp. NCPPB 2586]
ATGAGCACCATCGACTTCACCTCCTTCCTCAAGCTGATGGCGCACCAGAAGGCGTCGGATCTGTTCATCACCTCTGGCAT
GCCGCCGGCGATCAAGGTGCATGGCAAGATCAGCCCGATCACCCAGGCGCCGCTGACCGCGCAGCAGAGTCGCGACCTGG
TGTTGAACGTGATGACGCCCTCGCAGCGCGAGGAATTCGAAAAGACCCACGAGTGCAACTTCGCCATCGGCGTGTCCGGG
GTGGGGCGGTTCCGTGTGAGCTGCTTCTATCAGCGCAACCAGGTGGGAATGGTGCTGCGTCGGATCGAGACGCGCATTCC
CACCGTGGAGGAGCTGAGCCTGCCGCCGGTGATCAAGACACTGGCCATGACCAAGCGCGGCATCATCATCTTTGTCGGCG
CTACCGGTACCGGCAAGTCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAATTCCACCGGGCACATCATCACC
ATCGAAGACCCGATCGAATTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAAGTCGGCATCGATACCGATAG
CTGGGAAAACGCGCTGAAAAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGGACCCGCGAAGGCA
TGGACCATGCCATCGCCTTCGCCGAAACCGGCCACTTGGTGCTGTGCACGTTGCACGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCGGAAGACCGCCGCAACCAATTGCTGATGGACCTGTCGCTCAACCTCAAGGGTGTGGT
GGCGCAGCAGTTGATCCCGACCCCGGATGGCCGCGGCCGCCGCGTGGCGATGGAGATCATGCTGGGCACGCCGCTGGTGC
AGGATTACATCCGTGATGGGGAGATCCACAAGCTCAAGGAGATCATGAAGGAGTCCACCAACCTGGGCATGCGCACCTTC
GATCAGAGCCTGTTCGAGCTGTATCAGGCCGGCGAGATCAGCTACGAAGACGCACTGCGCTACGCCGACTCGCAGAACGA
GGTGCGCCTGCGCATCAAGCTCTCGCAAGGCGGCGATGCCAAGACGCTGTCGCAGGGGCTGGATGGCGTGGAGATTGCCG
AGGTTCGGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.302

97.606

0.657
  pilU Acinetobacter baylyi ADP1

63.585

94.947

0.604
  pilU Vibrio cholerae strain A1552

53.933

94.681

0.511
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.017

94.947

0.399
  pilT Acinetobacter baumannii strain A118

40.407

91.489

0.37
  pilT Acinetobacter nosocomialis M2

40.407

91.489

0.37
  pilT Acinetobacter baumannii D1279779

40.407

91.489

0.37
  pilT Pseudomonas aeruginosa PAK

41.194

89.096

0.367

Multiple sequence alignment