Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACCP99_RS07415 Genome accession   NZ_CP167849
Coordinates   1745836..1746966 (+) Length   376 a.a.
NCBI ID   WP_003489921.1    Uniprot ID   A0AAI7ZGP8
Organism   Xanthomonas sp. NCPPB 3443     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1740836..1751966
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACCP99_RS07390 (ACCP99_07390) - 1740931..1741572 (-) 642 WP_014090668.1 M23 family metallopeptidase -
  ACCP99_RS07395 (ACCP99_07395) - 1741679..1742104 (-) 426 WP_008571830.1 HU family DNA-binding protein -
  ACCP99_RS07400 (ACCP99_07400) proC 1742664..1743518 (-) 855 WP_372356914.1 pyrroline-5-carboxylate reductase -
  ACCP99_RS07405 (ACCP99_07405) - 1743562..1744254 (-) 693 WP_008571828.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACCP99_RS07410 (ACCP99_07410) - 1744672..1745723 (+) 1052 Protein_1460 type IV pilus twitching motility protein PilT -
  ACCP99_RS07415 (ACCP99_07415) pilU 1745836..1746966 (+) 1131 WP_003489921.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACCP99_RS07420 (ACCP99_07420) - 1747056..1748060 (-) 1005 WP_372356915.1 pectate lyase -
  ACCP99_RS07425 (ACCP99_07425) - 1748096..1748347 (-) 252 WP_162010796.1 hypothetical protein -
  ACCP99_RS07430 (ACCP99_07430) - 1748648..1749565 (-) 918 WP_372356916.1 DUF72 domain-containing protein -
  ACCP99_RS07435 (ACCP99_07435) - 1749573..1750145 (-) 573 WP_372356917.1 DNA-3-methyladenine glycosylase I -
  ACCP99_RS07440 (ACCP99_07440) - 1750422..1750988 (+) 567 WP_003489930.1 YqgE/AlgH family protein -
  ACCP99_RS07445 (ACCP99_07445) ruvX 1750981..1751448 (+) 468 WP_008571823.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 42044.26 Da        Isoelectric Point: 6.8286

>NTDB_id=1039832 ACCP99_RS07415 WP_003489921.1 1745836..1746966(+) (pilU) [Xanthomonas sp. NCPPB 3443]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVHGKISPITQTPLTAQQSRDLVLNVMTPSQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVDELSLPPVIKTLSMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGRSRRVAMEIMLGTPLVQDYIRDGEIHKLKEIMKESTNLGMRTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLSQGLDGVEISEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=1039832 ACCP99_RS07415 WP_003489921.1 1745836..1746966(+) (pilU) [Xanthomonas sp. NCPPB 3443]
ATGAGCACCATCGACTTCACCTCCTTCCTCAAGCTGATGGCGCATCAGAAGGCCTCGGACCTGTTCATCACCTCGGGCAT
GCCGCCGGCGATCAAGGTGCACGGCAAGATCAGCCCCATTACCCAGACCCCGCTGACTGCGCAGCAGAGCCGCGACCTGG
TGTTGAACGTGATGACGCCGTCGCAGCGCGAGGAATTCGAAAAGACCCACGAGTGCAATTTCGCCATCGGCGTATCCGGG
GTCGGGCGTTTCCGTGTGAGCTGCTTCTACCAGCGTAATCAGGTGGGCATGGTGCTGCGCCGGATCGAAACGCGCATCCC
CACCGTGGACGAGCTGAGCTTGCCGCCGGTGATCAAGACGCTGTCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
CCACCGGAACCGGTAAATCGACTTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAATTCCACCGGGCACATCATCACC
ATCGAAGATCCGATCGAATTCGTGCACAAGCACGAGGGTTGCATCATCACCCAGCGTGAGGTCGGCATCGACACCGACAG
CTGGGAGAACGCGTTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAAGGCA
TGGACCATGCGATCGCATTCGCCGAAACCGGCCATCTGGTGCTGTGCACGCTGCACGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCCGAAGATCGCCGCAACCAGCTGTTGATGGACCTGTCGCTCAACCTCAAGGGCGTGGT
CGCGCAGCAGCTGATTCCGACCCCGGACGGCCGCAGCCGTCGCGTGGCGATGGAAATCATGTTGGGCACGCCGCTGGTGC
AGGACTACATACGCGACGGCGAGATCCACAAGCTCAAGGAGATCATGAAGGAGTCCACCAACCTGGGCATGCGCACCTTC
GACCAGAGCCTGTTCGAGCTCTACCAGGCCGGCGAAATCAGTTACGAAGATGCGCTGCGCTACGCCGACTCGCAGAACGA
AGTACGCCTGCGCATCAAGCTCTCGCAGGGTGGCGATGCCAAGACCTTGTCGCAGGGCCTGGACGGCGTGGAAATTTCCG
AAGTGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.757

97.606

0.652
  pilU Acinetobacter baylyi ADP1

64.146

94.947

0.609
  pilU Vibrio cholerae strain A1552

52.809

94.681

0.5
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.457

94.947

0.394
  pilT Pseudomonas aeruginosa PAK

40.896

89.096

0.364
  pilT Acinetobacter nosocomialis M2

39.826

91.489

0.364
  pilT Acinetobacter baumannii D1279779

39.826

91.489

0.364
  pilT Acinetobacter baumannii strain A118

39.826

91.489

0.364

Multiple sequence alignment