Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACDI35_RS06245 Genome accession   NZ_CP167834
Coordinates   1408384..1410120 (+) Length   578 a.a.
NCBI ID   WP_372376105.1    Uniprot ID   -
Organism   Xanthomonas axonopodis pv. cajani strain NCPPB 2063     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1403384..1415120
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACDI35_RS06220 (ACDI35_06210) coaE 1404306..1404914 (-) 609 WP_022558665.1 dephospho-CoA kinase -
  ACDI35_RS06225 (ACDI35_06215) - 1404928..1405791 (-) 864 WP_003491180.1 A24 family peptidase -
  ACDI35_RS06230 (ACDI35_06220) pilC 1405798..1407057 (-) 1260 WP_033836749.1 type II secretion system F family protein Machinery gene
  ACDI35_RS06235 (ACDI35_06225) comP 1407403..1407831 (+) 429 WP_007962467.1 pilin Machinery gene
  ACDI35_RS06240 (ACDI35_06230) pilA2 1407929..1408342 (+) 414 WP_089111877.1 pilin Machinery gene
  ACDI35_RS06245 (ACDI35_06235) pilB 1408384..1410120 (+) 1737 WP_372376105.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACDI35_RS06250 (ACDI35_06240) pilR 1410570..1412024 (-) 1455 WP_022558668.1 sigma-54 dependent transcriptional regulator Regulator
  ACDI35_RS06255 (ACDI35_06245) - 1412294..1413907 (-) 1614 WP_372376106.1 PAS domain-containing sensor histidine kinase -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62646.73 Da        Isoelectric Point: 5.7586

>NTDB_id=1039688 ACDI35_RS06245 WP_372376105.1 1408384..1410120(+) (pilB) [Xanthomonas axonopodis pv. cajani strain NCPPB 2063]
MNVAIGSNLVGITGIARRLVQDGALDEVSARAAMDHATQAKVPLPQWFAEKKLVTAAQLAAANAVEFGMSLMDVSAFDAN
QNAVKLVSEELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEAILVDEDQIRRTLEQWQASNASLGSSLG
NDDEEMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEADQQK
LFLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
DNALLAEGFTPAQLAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQAIGIRDLRQSALM
KAAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=1039688 ACDI35_RS06245 WP_372376105.1 1408384..1410120(+) (pilB) [Xanthomonas axonopodis pv. cajani strain NCPPB 2063]
ATGAACGTTGCAATCGGCTCAAATCTAGTTGGAATTACTGGTATTGCGCGGCGCCTTGTTCAGGATGGTGCGCTTGATGA
GGTGAGCGCGCGTGCGGCAATGGATCACGCAACCCAGGCCAAAGTTCCTTTGCCGCAATGGTTCGCCGAAAAGAAGCTTG
TAACAGCCGCACAGCTCGCGGCCGCCAACGCAGTCGAGTTTGGCATGTCTTTGATGGATGTATCGGCATTCGACGCCAAC
CAGAACGCAGTCAAACTGGTCAGTGAGGAGTTGCTCCAGAAGCACCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGTT
GTTTGTGGGGGTGAGCAACCCGACCCAGACCCGGGCGCTGGACGACATCAAGTTCCATACGAACCTGGTGGTCGAAGCCA
TTCTTGTCGACGAGGATCAGATCCGCCGGACGCTCGAACAGTGGCAGGCCAGCAATGCGTCGTTGGGTTCGTCGCTTGGC
AACGACGACGAGGAAATGGGGGATCTGGACGTTTCGGCTGGCGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCGTCGGACA
TCCATTTCGAGCCGTATGAAGACGACTACCGGGTGCGCTTGCGCATCGATGGCCTGTTGAAGAATGTGGCCAAGGCGCCG
GTGAAGCTGAACCAGCGCATCGCGGCGCGCCTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTCAGCACCTTGCCGACCCTGTTCGGCGAGA
AGGTGGTGCTGCGTATCCTGGACGGCAGTGCGGCCAAGCTGGGCATCGACAAGCTGGGCTATGAGGCAGACCAGCAGAAG
TTGTTCCTGGAGGCGATCCACAAGCCCTATGGCATGGTGCTGGTGACCGGGCCGACCGGCTCGGGCAAGACGGTGTCGTT
GTATACGGCGCTGGGAATTCTCAACGACGAGACGCGCAATATCTCCACCGCGGAGGATCCGGTCGAAATCCGCTTGCCTG
GCGTCAATCAGGTACAGCAGAACAACAAGCGTGGCATGACCTTCGCGGCAGCCTTGCGCTCGTTCCTGCGACAGGACCCG
GACATCATCATGGTCGGCGAAATCCGTGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACGGGTCACATGGT
GCTGTCGACGTTGCACACCAACGATGCGCCGCAGACCATCGCCCGTTTGATGAACATGGGCATTGCGCCCTACAACATCA
CCTCGTCAGTGACGCTGGTGATCGCGCAGCGTCTGGCGCGGCGGTTGTGCAACAACTGCAAGCGCAAGTCGACGCTTCCT
GACAACGCATTGCTGGCCGAAGGGTTCACGCCTGCACAGCTTGCCGCCGGGATCGAGCTGTATGAGGCGGTCGGTTGCGA
CGAGTGCACCGAAGGCTACAAGGGGCGTACCGGTATCTACCAGGTGATGCCGATGACCGACGAAATCGGCGCGATCGTGC
TGGAAGGCGGCAACGCGATGCAGATCGCCGAGGCCGCGCAGGCGATCGGTATCCGCGATTTGCGGCAGTCGGCGTTGATG
AAGGCTGCGCACGGGGTGACCAGCCTGGCCGAGATCAATCGTGTGACGAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-153)

Type II/IV secretion system protein (T2SSE)

(201-468)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.81

98.27

0.548
  pilB Acinetobacter baylyi ADP1

54.93

98.27

0.54
  pilB Legionella pneumophila strain ERS1305867

51.384

100

0.514
  pilB Vibrio cholerae strain A1552

48.616

100

0.486
  pilF Neisseria gonorrhoeae MS11

49.296

98.27

0.484
  pilB Vibrio parahaemolyticus RIMD 2210633

46.607

96.886

0.452
  pilB Vibrio campbellii strain DS40M4

45.343

98.443

0.446

Multiple sequence alignment